Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes

Dermatophytes are fungi responsible for causing superficial infections. In patients with diabetes mellitus (DM), dermatophytosis is usually more severe and recurrent. In the present study, we aimed to investigate the influence of short and long term hypoinsulinemia-hyperglycemia (HH) during experime...

Full description

Saved in:
Bibliographic Details
Main Authors: Thais F. C. Fraga-Silva, Camila M. Marchetti, Luiza A. N. Mimura, Gisele A. Locachevic, Márjorie A. Golim, James Venturini, Maria S. P. Arruda
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2015/342345
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850120790369894400
author Thais F. C. Fraga-Silva
Camila M. Marchetti
Luiza A. N. Mimura
Gisele A. Locachevic
Márjorie A. Golim
James Venturini
Maria S. P. Arruda
author_facet Thais F. C. Fraga-Silva
Camila M. Marchetti
Luiza A. N. Mimura
Gisele A. Locachevic
Márjorie A. Golim
James Venturini
Maria S. P. Arruda
author_sort Thais F. C. Fraga-Silva
collection DOAJ
description Dermatophytes are fungi responsible for causing superficial infections. In patients with diabetes mellitus (DM), dermatophytosis is usually more severe and recurrent. In the present study, we aimed to investigate the influence of short and long term hypoinsulinemia-hyperglycemia (HH) during experimental infection by Trichophyton mentagrophytes as well as alterations in the mononuclear phagocytes. Our results showed two distinct profiles of fungal outcome and immune response. Short term HH induced a discrete impaired proinflammatory response by peritoneal adherent cells (PAC) and a delayed fungal clearance. Moreover, long term HH mice showed low and persistent fungal load and a marked reduction in the production of TNF-α by PAC. Furthermore, while the inoculation of TM in non-HH mice triggered high influx of Gr1+ monocytes into the peripheral blood, long term HH mice showed low percentage of these cells. Thus, our results demonstrate that the time of exposure of HH interferes with the TM infection outcome as well as the immunobiology of mononuclear phagocytes, including fresh monocyte recruitment from bone marrow and PAC activity.
format Article
id doaj-art-63c2a0591a3d44df915da5ef6eee7618
institution OA Journals
issn 0962-9351
1466-1861
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Mediators of Inflammation
spelling doaj-art-63c2a0591a3d44df915da5ef6eee76182025-08-20T02:35:16ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/342345342345Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear PhagocytesThais F. C. Fraga-Silva0Camila M. Marchetti1Luiza A. N. Mimura2Gisele A. Locachevic3Márjorie A. Golim4James Venturini5Maria S. P. Arruda6Department of Biological Sciences, School of Sciences, Universidade Estadual Paulista (UNESP), 17033-360 Bauru, SP, BrazilDepartment of Biological Sciences, School of Sciences, Universidade Estadual Paulista (UNESP), 17033-360 Bauru, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, BrazilDepartment of Clinical, Toxicological and Bromatological Analysis, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), 14040-903 Ribeirão Preto, SP, BrazilBotucatu Blood Center, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, BrazilDepartment of Biological Sciences, School of Sciences, Universidade Estadual Paulista (UNESP), 17033-360 Bauru, SP, BrazilDepartment of Biological Sciences, School of Sciences, Universidade Estadual Paulista (UNESP), 17033-360 Bauru, SP, BrazilDermatophytes are fungi responsible for causing superficial infections. In patients with diabetes mellitus (DM), dermatophytosis is usually more severe and recurrent. In the present study, we aimed to investigate the influence of short and long term hypoinsulinemia-hyperglycemia (HH) during experimental infection by Trichophyton mentagrophytes as well as alterations in the mononuclear phagocytes. Our results showed two distinct profiles of fungal outcome and immune response. Short term HH induced a discrete impaired proinflammatory response by peritoneal adherent cells (PAC) and a delayed fungal clearance. Moreover, long term HH mice showed low and persistent fungal load and a marked reduction in the production of TNF-α by PAC. Furthermore, while the inoculation of TM in non-HH mice triggered high influx of Gr1+ monocytes into the peripheral blood, long term HH mice showed low percentage of these cells. Thus, our results demonstrate that the time of exposure of HH interferes with the TM infection outcome as well as the immunobiology of mononuclear phagocytes, including fresh monocyte recruitment from bone marrow and PAC activity.http://dx.doi.org/10.1155/2015/342345
spellingShingle Thais F. C. Fraga-Silva
Camila M. Marchetti
Luiza A. N. Mimura
Gisele A. Locachevic
Márjorie A. Golim
James Venturini
Maria S. P. Arruda
Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
Mediators of Inflammation
title Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
title_full Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
title_fullStr Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
title_full_unstemmed Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
title_short Relationship among Short and Long Term of Hypoinsulinemia-Hyperglycemia, Dermatophytosis, and Immunobiology of Mononuclear Phagocytes
title_sort relationship among short and long term of hypoinsulinemia hyperglycemia dermatophytosis and immunobiology of mononuclear phagocytes
url http://dx.doi.org/10.1155/2015/342345
work_keys_str_mv AT thaisfcfragasilva relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT camilammarchetti relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT luizaanmimura relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT giselealocachevic relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT marjorieagolim relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT jamesventurini relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes
AT mariasparruda relationshipamongshortandlongtermofhypoinsulinemiahyperglycemiadermatophytosisandimmunobiologyofmononuclearphagocytes