Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135
Dibenzothiophene (DBT) is a widespread environmental pollutant. The most common metabolic pathway for DBT degradation by <i>Gordonia</i> strains is the 4S pathway, which is under the control of the <i>dsz</i> operon. The ability to utilize DBT as the sole source of sulfur in...
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2025-02-01
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| author | Ekaterina Frantsuzova Alexander Bogun Anna Vetrova Elizaveta Kazakova Tomiris Kusainova Irina Tarasova Irina Pozdnyakova-Filatova Yanina Delegan |
| author_facet | Ekaterina Frantsuzova Alexander Bogun Anna Vetrova Elizaveta Kazakova Tomiris Kusainova Irina Tarasova Irina Pozdnyakova-Filatova Yanina Delegan |
| author_sort | Ekaterina Frantsuzova |
| collection | DOAJ |
| description | Dibenzothiophene (DBT) is a widespread environmental pollutant. The most common metabolic pathway for DBT degradation by <i>Gordonia</i> strains is the 4S pathway, which is under the control of the <i>dsz</i> operon. The ability to utilize DBT as the sole source of sulfur in <i>Gordonia alkanivorans</i> strain 135 has been revealed. The <i>dsz</i> operon was not detected in the genome of strain 135. In this work, using genomic, transcriptomic, and proteomic data of strain 135, it was shown that an alternative pathway of DBT transformation is possible in non-<i>dsz Gordonia</i>; the <i>sfn</i>B and <i>tau</i>D genes and two acyl-dehydrogenase genes are significantly involved in the desulfurization process. |
| format | Article |
| id | doaj-art-63bfaf2978d94a89b86f42fa0013a0b3 |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj-art-63bfaf2978d94a89b86f42fa0013a0b32025-08-20T02:44:31ZengMDPI AGBiology2079-77372025-02-0114218810.3390/biology14020188Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135Ekaterina Frantsuzova0Alexander Bogun1Anna Vetrova2Elizaveta Kazakova3Tomiris Kusainova4Irina Tarasova5Irina Pozdnyakova-Filatova6Yanina Delegan7Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of Russian Academy of Sciences” (FRC PSCBR RAS), Pushchino 142290, RussiaInstitute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of Russian Academy of Sciences” (FRC PSCBR RAS), Pushchino 142290, RussiaInstitute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of Russian Academy of Sciences” (FRC PSCBR RAS), Pushchino 142290, RussiaV. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow 119334, RussiaV. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow 119334, RussiaV. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow 119334, RussiaInstitute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of Russian Academy of Sciences” (FRC PSCBR RAS), Pushchino 142290, RussiaInstitute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of Russian Academy of Sciences” (FRC PSCBR RAS), Pushchino 142290, RussiaDibenzothiophene (DBT) is a widespread environmental pollutant. The most common metabolic pathway for DBT degradation by <i>Gordonia</i> strains is the 4S pathway, which is under the control of the <i>dsz</i> operon. The ability to utilize DBT as the sole source of sulfur in <i>Gordonia alkanivorans</i> strain 135 has been revealed. The <i>dsz</i> operon was not detected in the genome of strain 135. In this work, using genomic, transcriptomic, and proteomic data of strain 135, it was shown that an alternative pathway of DBT transformation is possible in non-<i>dsz Gordonia</i>; the <i>sfn</i>B and <i>tau</i>D genes and two acyl-dehydrogenase genes are significantly involved in the desulfurization process.https://www.mdpi.com/2079-7737/14/2/188<i>Gordonia</i>biodegradationaromatic compoundstranscriptomicsproteomics |
| spellingShingle | Ekaterina Frantsuzova Alexander Bogun Anna Vetrova Elizaveta Kazakova Tomiris Kusainova Irina Tarasova Irina Pozdnyakova-Filatova Yanina Delegan Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 Biology <i>Gordonia</i> biodegradation aromatic compounds transcriptomics proteomics |
| title | Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 |
| title_full | Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 |
| title_fullStr | Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 |
| title_full_unstemmed | Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 |
| title_short | Integrated Omics Approaches to Explore a New System of Genetic Control of Dibenzothiophene Desulfurization and Aromatic Ring Cleavage by <i>Gordonia alkanivorans</i> Strain 135 |
| title_sort | integrated omics approaches to explore a new system of genetic control of dibenzothiophene desulfurization and aromatic ring cleavage by i gordonia alkanivorans i strain 135 |
| topic | <i>Gordonia</i> biodegradation aromatic compounds transcriptomics proteomics |
| url | https://www.mdpi.com/2079-7737/14/2/188 |
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