Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis
Abstract Rehmannia chingii (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we p...
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60909-9 |
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| author | Fengqing Wang Zhennan Jiang Junge Gao Chunyan Miao Ci Song Yahe Yang Ning Ding Yajing Li Hongzheng Sun Caixia Xie Bao Zhang Pengyu Zhang Li Gu Qiuwen Hao Zhongyi Zhang Luqi Huang Yuan Yuan |
| author_facet | Fengqing Wang Zhennan Jiang Junge Gao Chunyan Miao Ci Song Yahe Yang Ning Ding Yajing Li Hongzheng Sun Caixia Xie Bao Zhang Pengyu Zhang Li Gu Qiuwen Hao Zhongyi Zhang Luqi Huang Yuan Yuan |
| author_sort | Fengqing Wang |
| collection | DOAJ |
| description | Abstract Rehmannia chingii (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we present the 1.169 Gb telomere-to-telomere (T2T) genome sequence of R. chingii. Phylogenetic analysis confirms that Rehmannia belongs to the Orobanchaceae family. We find that structural genes of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway and the iridoid pathway are predominantly expressed in R. chingii leaves. Further analyses reveal a cytochrome P450 gene cluster localized on chromosome 8, and identify RcCYP72H7 within this cluster as an aucubin epoxidase, capable of catalyzing aucubin epoxidation to form catalpol. The genome offers valuable resources for studying iridoid glycoside biosynthesis and the evolutionary history of Rehmannia, and will help to faciliate genetic improvement of R. chingii for pharmaceutical and health-related applications. |
| format | Article |
| id | doaj-art-63b54ebcd0084c1da2a2ec5be2ea9017 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-63b54ebcd0084c1da2a2ec5be2ea90172025-08-20T04:01:41ZengNature PortfolioNature Communications2041-17232025-07-0116111710.1038/s41467-025-60909-9Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesisFengqing Wang0Zhennan Jiang1Junge Gao2Chunyan Miao3Ci Song4Yahe Yang5Ning Ding6Yajing Li7Hongzheng Sun8Caixia Xie9Bao Zhang10Pengyu Zhang11Li Gu12Qiuwen Hao13Zhongyi Zhang14Luqi Huang15Yuan Yuan16College of Agronomy, Henan Agricultural UniversityState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Experimental Research Center, China Academy of Chinese Medical Sciences (CACMS)College of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineCollege of Agronomy, Henan Agricultural UniversitySchool of Medicine, Henan University of Chinese MedicineSchool of Medicine, Henan University of Chinese MedicineCollege of Agronomy, Henan Agricultural UniversityCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry UniversityState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Experimental Research Center, China Academy of Chinese Medical Sciences (CACMS)State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Experimental Research Center, China Academy of Chinese Medical Sciences (CACMS)Abstract Rehmannia chingii (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we present the 1.169 Gb telomere-to-telomere (T2T) genome sequence of R. chingii. Phylogenetic analysis confirms that Rehmannia belongs to the Orobanchaceae family. We find that structural genes of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway and the iridoid pathway are predominantly expressed in R. chingii leaves. Further analyses reveal a cytochrome P450 gene cluster localized on chromosome 8, and identify RcCYP72H7 within this cluster as an aucubin epoxidase, capable of catalyzing aucubin epoxidation to form catalpol. The genome offers valuable resources for studying iridoid glycoside biosynthesis and the evolutionary history of Rehmannia, and will help to faciliate genetic improvement of R. chingii for pharmaceutical and health-related applications.https://doi.org/10.1038/s41467-025-60909-9 |
| spellingShingle | Fengqing Wang Zhennan Jiang Junge Gao Chunyan Miao Ci Song Yahe Yang Ning Ding Yajing Li Hongzheng Sun Caixia Xie Bao Zhang Pengyu Zhang Li Gu Qiuwen Hao Zhongyi Zhang Luqi Huang Yuan Yuan Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis Nature Communications |
| title | Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis |
| title_full | Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis |
| title_fullStr | Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis |
| title_full_unstemmed | Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis |
| title_short | Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis |
| title_sort | analysis of the rehmannia chingii geneome identifies rccyp72h7 as an epoxidase in iridoid glycoside biosynthesis |
| url | https://doi.org/10.1038/s41467-025-60909-9 |
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