Simplified method for purifying full-length major type IV pilins: PilA from Acidithiobacillus thiooxidans

Simplified method for purifying full-length major type IV pilins: PilA from Acidithiobacillus thiooxidans

Type IV major pilins are membrane-associated proteins that play critical roles in bacterial adaptation and survival across diverse environmental conditions, including potentially extreme ones. However, their expression and purification remain challenging due to their hydrophobic nature and tendency...

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Bibliographic Details
Main Authors: Edgar D. Páez-Pérez, Araceli Hernández-Sánchez, Elvia Alfaro-Saldaña, J. Viridiana García-Meza
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:MethodsX
Subjects:
Major pilin
Minor pilin
Acidophile
Protein expression
Protein purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016125003644
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