Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves
Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 1...
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| Format: | Article |
| Language: | English |
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Sciendo
2014-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2014-0033 |
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| author | Salazar Marcos Chérigo Lilia Acosta Hildaura Otero Rafael Martínez-Luis Sergio |
| author_facet | Salazar Marcos Chérigo Lilia Acosta Hildaura Otero Rafael Martínez-Luis Sergio |
| author_sort | Salazar Marcos |
| collection | DOAJ |
| description | Significant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 μg of venom kg-1 b.m.) lowered the hemorrhagic activity of the venom alone in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L-1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to the isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HRMS and NMR (1H, 13C, HSQC, HMBC and COSY). A quercetrin concentration of 0.11 μmol L-1 prolonged the plasma coagulation time 2.6 times demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract. |
| format | Article |
| id | doaj-art-63af20757e7149998d2e934306179f02 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-63af20757e7149998d2e934306179f022025-02-02T00:31:54ZengSciendoActa Pharmaceutica1846-95582014-12-0164447548310.2478/acph-2014-0033acph-2014-0033Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leavesSalazar Marcos0Chérigo Lilia1Acosta Hildaura2Otero Rafael3Martínez-Luis Sergio4Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET) Facultad de Medicina Universidad de PanamáFacultad de Ciencias Naturales Exactas y Tecnología Universidad de PanamáCentro de Investigación e Información de Medicamentos y Tóxicos (CIIMET) Facultad de Medicina Universidad de PanamáFacultad de Medicina Universidad de Antioquia Medellín, ColombiaCenter for Biodiversity and Drug Discovery INDICASAT-AIP, Panama, PanamaSignificant inhibition of the coagulant and hemorrhagic effects of Bothrops asper venom was demonstrated by ethanolic extract prepared from the leaves of Brownea rosademonte. In vitro experiments preincubating 5.5 mg of extract kg-1 b.m. for 30 min with a minimum hemorrhagic dose of venom (273.8 ± 16.1 μg of venom kg-1 b.m.) lowered the hemorrhagic activity of the venom alone in CD-1 mice by 51.5 ± 2.6 %. Additionally, 1.7 mg extract L-1 plasma prolonged 5.1 times the plasma coagulation time. Fractionation of the extract led to the isolation of two compounds: ononitol (1) and quercetrin (2). The structure of compounds 1 and 2 was established by spectroscopic analyses, including APCI-HRMS and NMR (1H, 13C, HSQC, HMBC and COSY). A quercetrin concentration of 0.11 μmol L-1 prolonged the plasma coagulation time 2.6 times demonstrating that this compound was one of the active constituents of the Brownea rosademonte extract.https://doi.org/10.2478/acph-2014-0033bothrops asper (viperidae) venombrownea rosademonte (caesalpinaceae)quercetrinanticoagulantantihemorrhagicantiproteolyticantihemolytic |
| spellingShingle | Salazar Marcos Chérigo Lilia Acosta Hildaura Otero Rafael Martínez-Luis Sergio Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves Acta Pharmaceutica bothrops asper (viperidae) venom brownea rosademonte (caesalpinaceae) quercetrin anticoagulant antihemorrhagic antiproteolytic antihemolytic |
| title | Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves |
| title_full | Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves |
| title_fullStr | Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves |
| title_full_unstemmed | Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves |
| title_short | Evaluation of anti-Bothrops asper venom activity of ethanolic extract of Brownea rosademonte leaves |
| title_sort | evaluation of anti bothrops asper venom activity of ethanolic extract of brownea rosademonte leaves |
| topic | bothrops asper (viperidae) venom brownea rosademonte (caesalpinaceae) quercetrin anticoagulant antihemorrhagic antiproteolytic antihemolytic |
| url | https://doi.org/10.2478/acph-2014-0033 |
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