Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon
Introduction. Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene exp...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2018/8530318 |
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| author | Ghina B. Fakhri Reem S. Akel Maya K. Khalil Deborah A. Mukherji Fouad I. Boulos Arafat H. Tfayli |
| author_facet | Ghina B. Fakhri Reem S. Akel Maya K. Khalil Deborah A. Mukherji Fouad I. Boulos Arafat H. Tfayli |
| author_sort | Ghina B. Fakhri |
| collection | DOAJ |
| description | Introduction. Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens. Methods. Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc). Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. Concordance rates, agreement measures, and kappa scores were calculated for both methods. Results. For ER, Kappa score was 0.918 (95% CI, 0.77.3–1.000) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). For PR, Kappa score was 0.652 (95% CI, 0.405–0.849) and concordance rate was 86.4% (95% CI, 77%–93%). For HER2, Kappa score was 0.709 (95% CI, 0.428–0.916) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). Conclusion. Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor. In general, microarray gene expression and IHC proved to have high concordance rates. Several factors can increase the discordance rate such as differences in sample processing. |
| format | Article |
| id | doaj-art-63a90306753e4ce98b608497e7e82d2d |
| institution | Kabale University |
| issn | 2090-3170 2090-3189 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-63a90306753e4ce98b608497e7e82d2d2025-02-03T05:57:12ZengWileyInternational Journal of Breast Cancer2090-31702090-31892018-01-01201810.1155/2018/85303188530318Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in LebanonGhina B. Fakhri0Reem S. Akel1Maya K. Khalil2Deborah A. Mukherji3Fouad I. Boulos4Arafat H. Tfayli5Division of Hematology and Oncology, Department of Internal Medicine, American University of Beirut, Beirut, LebanonDivision of Hematology and Oncology, Department of Internal Medicine, American University of Beirut, Beirut, LebanonRoswell Park Comprehensive Cancer Center/ State University at Buffalo-Jacobs School of Medicine and Biomedical Sciences, Elm & Carlton Streets, Buffalo, NY 14263, USADivision of Hematology and Oncology, Department of Internal Medicine, American University of Beirut, Beirut, LebanonDepartment of Pathology and Laboratory Medicine, American University of Beirut, Beirut, LebanonDivision of Hematology and Oncology, Department of Internal Medicine, American University of Beirut, Beirut, LebanonIntroduction. Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens. Methods. Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc). Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. Concordance rates, agreement measures, and kappa scores were calculated for both methods. Results. For ER, Kappa score was 0.918 (95% CI, 0.77.3–1.000) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). For PR, Kappa score was 0.652 (95% CI, 0.405–0.849) and concordance rate was 86.4% (95% CI, 77%–93%). For HER2, Kappa score was 0.709 (95% CI, 0.428–0.916) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). Conclusion. Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor. In general, microarray gene expression and IHC proved to have high concordance rates. Several factors can increase the discordance rate such as differences in sample processing.http://dx.doi.org/10.1155/2018/8530318 |
| spellingShingle | Ghina B. Fakhri Reem S. Akel Maya K. Khalil Deborah A. Mukherji Fouad I. Boulos Arafat H. Tfayli Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon International Journal of Breast Cancer |
| title | Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon |
| title_full | Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon |
| title_fullStr | Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon |
| title_full_unstemmed | Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon |
| title_short | Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon |
| title_sort | concordance between immunohistochemistry and microarray gene expression profiling for estrogen receptor progesterone receptor and her2 receptor statuses in breast cancer patients in lebanon |
| url | http://dx.doi.org/10.1155/2018/8530318 |
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