Producing peptide arrays for epitope mapping by intein-mediated protein ligation

Peptide arrays are increasingly used to define antibody epitopes and substrate specificities of protein kinases. Their use is hampered, however, by ineffective and variable binding efficiency of peptides, which often results in low sensitivity and inconsistent results. To overcome these limitations,...

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Bibliographic Details
Main Authors: Luo Sun, John Rush, Inca Ghosh, Jeremy R. Maunus, Ming-Qun Xu
Format: Article
Language:English
Published: Taylor & Francis Group 2004-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/04373RR01
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