Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease

Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease

<i>Clostridium perfringens</i> infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm o...

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Main Authors: Wenjing Zhang, Zhenzhen Hao, Daoxin Yang, Wangli Ji, Kairui Guo, Xianhua Sun, Shuai Wang, Shuyan Yang, Jianshuang Ma, Tong Wang, Huiying Luo, Bin Yao, Meiling Zhang, Yuan Wang, Huoqing Huang, Xiaoyun Su
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Animals
Subjects:
necrotic enteritis
<i>Clostridium perfringens</i>
xylanase
protease
broiler chickens
gut microbiota modulation
Online Access:https://www.mdpi.com/2076-2615/15/2/123
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Summary:<i>Clostridium perfringens</i> infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of <i>C. perfringens,</i> were analyzed for their ability to alleviate <i>C. perfringens</i>-induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no <i>C. perfringens</i> challenge; Cp, no enzyme, <i>C. perfringens</i> challenge; Xyn, <i>Cb</i>Xyn10C plus <i>C. perfringens</i> challenge; Xyn+Am, <i>Cb</i>Xyn10C+Amylase plus <i>C. perfringens</i> challenge; Xyn+Ap, <i>Cb</i>Xyn10C+Alkaline protease plus <i>C. perfringens</i> challenge). The <i>C. perfringens</i> CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with <i>C. perfringens</i> (<i>p</i> < 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of <i>Lactobacillus</i> and <i>Butyricicoccus</i> bacteria in the intestine. These results indicated that <i>Cb</i>Xyn10C and protease can be used to alleviate intestinal damage caused by <i>C. perfringens</i> infection.
ISSN:2076-2615

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