Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves
Purpose. Our physiopathological assumption is that u-PA, t-PA, and PAI-1 are released by calcified aortic valves and play a role in the calcification of these valves. Methods. Sixty-five calcified aortic valves were collected from patients suffering from aortic stenosis. Each valve was incubated for...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2014/658643 |
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| author | Najlah Kochtebane Abdullah Mossa M. Alzahrani Aghleb Bartegi |
| author_facet | Najlah Kochtebane Abdullah Mossa M. Alzahrani Aghleb Bartegi |
| author_sort | Najlah Kochtebane |
| collection | DOAJ |
| description | Purpose. Our physiopathological assumption is that u-PA, t-PA, and PAI-1 are released by calcified aortic valves and play a role in the calcification of these valves. Methods. Sixty-five calcified aortic valves were collected from patients suffering from aortic stenosis. Each valve was incubated for 24 hours in culture medium. The supernatants were used to measure u-PA, t-PA, and PAI-1 concentrations; the valve calcification was evaluated using biphotonic absorptiometry. Results. Aortic stenosis valves expressed normal plasminogen activators concentrations and overexpressed PAI-1 (u-PA, t-PA, and PAI-1 mean concentrations were, resp., 1.69 ng/mL ± 0.80, 2.76 ng/mL ± 1.33, and 53.27 ng/mL ± 36.39). There was no correlation between u-PA and PAI-1 (r=0.3) but t-PA and PAI-1 were strongly correlated with each other (r=0.6). Overexpression of PAI-1 was proportional to the calcium content of the AS valves. Conclusions. Our results demonstrate a consistent increase of PAI-1 proportional to the calcification. The overexpression of PAI-1 may be useful as a predictive indicator in patients with aortic stenosis. |
| format | Article |
| id | doaj-art-639dc16dd8aa4e8db7c6504ea5f3a175 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-639dc16dd8aa4e8db7c6504ea5f3a1752025-08-20T03:34:29ZengWileyBiochemistry Research International2090-22472090-22552014-01-01201410.1155/2014/658643658643Expression of uPA, tPA, and PAI-1 in Calcified Aortic ValvesNajlah Kochtebane0Abdullah Mossa M. Alzahrani1Aghleb Bartegi2Laboratoire Cardiovasculaire, Hôpital Bichat, Faculté de Medecine, INSERM, Paris, FranceDepartment of Biology, Faculty of Sciences, King Faical University, P.O. Box 400, Hofuf, Al Hasa, Saudi ArabiaDepartment of Biology, Faculty of Sciences, King Faical University, P.O. Box 400, Hofuf, Al Hasa, Saudi ArabiaPurpose. Our physiopathological assumption is that u-PA, t-PA, and PAI-1 are released by calcified aortic valves and play a role in the calcification of these valves. Methods. Sixty-five calcified aortic valves were collected from patients suffering from aortic stenosis. Each valve was incubated for 24 hours in culture medium. The supernatants were used to measure u-PA, t-PA, and PAI-1 concentrations; the valve calcification was evaluated using biphotonic absorptiometry. Results. Aortic stenosis valves expressed normal plasminogen activators concentrations and overexpressed PAI-1 (u-PA, t-PA, and PAI-1 mean concentrations were, resp., 1.69 ng/mL ± 0.80, 2.76 ng/mL ± 1.33, and 53.27 ng/mL ± 36.39). There was no correlation between u-PA and PAI-1 (r=0.3) but t-PA and PAI-1 were strongly correlated with each other (r=0.6). Overexpression of PAI-1 was proportional to the calcium content of the AS valves. Conclusions. Our results demonstrate a consistent increase of PAI-1 proportional to the calcification. The overexpression of PAI-1 may be useful as a predictive indicator in patients with aortic stenosis.http://dx.doi.org/10.1155/2014/658643 |
| spellingShingle | Najlah Kochtebane Abdullah Mossa M. Alzahrani Aghleb Bartegi Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves Biochemistry Research International |
| title | Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves |
| title_full | Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves |
| title_fullStr | Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves |
| title_full_unstemmed | Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves |
| title_short | Expression of uPA, tPA, and PAI-1 in Calcified Aortic Valves |
| title_sort | expression of upa tpa and pai 1 in calcified aortic valves |
| url | http://dx.doi.org/10.1155/2014/658643 |
| work_keys_str_mv | AT najlahkochtebane expressionofupatpaandpai1incalcifiedaorticvalves AT abdullahmossamalzahrani expressionofupatpaandpai1incalcifiedaorticvalves AT aghlebbartegi expressionofupatpaandpai1incalcifiedaorticvalves |