Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients
Recent studies have shown that microRNA-16-5p (miR-16-5p) plays a crucial role in the pathological mechanism of vascular calcification. Nevertheless, the expression profile of miR-16-5p in maintenance hemodialysis (MHD) patients who are predisposed to vascular calcification remains unknown. This stu...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Renal Failure |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2368091 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850109149044539392 |
|---|---|
| author | Qiaojing Liang Chen Fu Yingjie Liu Wenhu Liu Weikang Guo |
| author_facet | Qiaojing Liang Chen Fu Yingjie Liu Wenhu Liu Weikang Guo |
| author_sort | Qiaojing Liang |
| collection | DOAJ |
| description | Recent studies have shown that microRNA-16-5p (miR-16-5p) plays a crucial role in the pathological mechanism of vascular calcification. Nevertheless, the expression profile of miR-16-5p in maintenance hemodialysis (MHD) patients who are predisposed to vascular calcification remains unknown. This study aims to investigate the potential associations between calcification risk and serum miR-16-5p expression among MHD patients. This cross-sectional study involved 132 MHD patients from the Dialysis Center of Beijing Friendship Hospital between 1 January 2019 and 31 December 2020. The degree of calcification in MHD patients was assessed using the Abdominal aortic calcification (AAC) score, and miR-16-5p expression was quantified using quantitative real-time polymerase chain reaction (qRT-PCR) with the 2–ΔΔCT method. Statistical analyses, including spearman correlation, linear regression and logistic regression analysis were used to explore the associations between laboratory parameters and AAC score. Calcifications were observed in 79(59.80%) patients. The linear regression showed a one-quartile decrease in miR-16-5p expression led to a significant increase in the AAC score by 5.336 (95% CI: 2.670–10.662, p = 0.000). Multivariate logistic regression analyses revealed that decreased miR-16-5p expression, reduced serum urea nitrogen, elevated white blood cell count, and longer dialysis vintage were significantly associated with an increased incidence of vascular calcification. The Area Under the Curve (AUC) of the Receiver Operating Characteristic (ROC) of the miR-16-5p-based logistic regression model was 0.842 (95% CI: 0.771–0.913, p = 0.000). There was an independent association between miR-16-5p expression and calcification degree. Lower miR-16-5p expression levels seem to be a potential risk factor of vascular calcification in MHD patients. |
| format | Article |
| id | doaj-art-638f044a2b50437da23139a34e2163d2 |
| institution | OA Journals |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-638f044a2b50437da23139a34e2163d22025-08-20T02:38:10ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2368091Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patientsQiaojing Liang0Chen Fu1Yingjie Liu2Wenhu Liu3Weikang Guo4Department of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, ChinaDepartment of Nephrology, Beijing Jishuitan Hospital, Capital Medical University, Beijing, ChinaDepartment of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, ChinaDepartment of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, ChinaDepartment of Nephrology, Faculty of Kidney Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, ChinaRecent studies have shown that microRNA-16-5p (miR-16-5p) plays a crucial role in the pathological mechanism of vascular calcification. Nevertheless, the expression profile of miR-16-5p in maintenance hemodialysis (MHD) patients who are predisposed to vascular calcification remains unknown. This study aims to investigate the potential associations between calcification risk and serum miR-16-5p expression among MHD patients. This cross-sectional study involved 132 MHD patients from the Dialysis Center of Beijing Friendship Hospital between 1 January 2019 and 31 December 2020. The degree of calcification in MHD patients was assessed using the Abdominal aortic calcification (AAC) score, and miR-16-5p expression was quantified using quantitative real-time polymerase chain reaction (qRT-PCR) with the 2–ΔΔCT method. Statistical analyses, including spearman correlation, linear regression and logistic regression analysis were used to explore the associations between laboratory parameters and AAC score. Calcifications were observed in 79(59.80%) patients. The linear regression showed a one-quartile decrease in miR-16-5p expression led to a significant increase in the AAC score by 5.336 (95% CI: 2.670–10.662, p = 0.000). Multivariate logistic regression analyses revealed that decreased miR-16-5p expression, reduced serum urea nitrogen, elevated white blood cell count, and longer dialysis vintage were significantly associated with an increased incidence of vascular calcification. The Area Under the Curve (AUC) of the Receiver Operating Characteristic (ROC) of the miR-16-5p-based logistic regression model was 0.842 (95% CI: 0.771–0.913, p = 0.000). There was an independent association between miR-16-5p expression and calcification degree. Lower miR-16-5p expression levels seem to be a potential risk factor of vascular calcification in MHD patients.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2368091Vascular calcificationhemodialysischronic kidney diseasemicroRNArenal failure |
| spellingShingle | Qiaojing Liang Chen Fu Yingjie Liu Wenhu Liu Weikang Guo Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients Renal Failure Vascular calcification hemodialysis chronic kidney disease microRNA renal failure |
| title | Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients |
| title_full | Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients |
| title_fullStr | Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients |
| title_full_unstemmed | Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients |
| title_short | Association of plasma microRNA-16-5p and abdominal aortic calcification in maintenance hemodialysis patients |
| title_sort | association of plasma microrna 16 5p and abdominal aortic calcification in maintenance hemodialysis patients |
| topic | Vascular calcification hemodialysis chronic kidney disease microRNA renal failure |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2368091 |
| work_keys_str_mv | AT qiaojingliang associationofplasmamicrorna165pandabdominalaorticcalcificationinmaintenancehemodialysispatients AT chenfu associationofplasmamicrorna165pandabdominalaorticcalcificationinmaintenancehemodialysispatients AT yingjieliu associationofplasmamicrorna165pandabdominalaorticcalcificationinmaintenancehemodialysispatients AT wenhuliu associationofplasmamicrorna165pandabdominalaorticcalcificationinmaintenancehemodialysispatients AT weikangguo associationofplasmamicrorna165pandabdominalaorticcalcificationinmaintenancehemodialysispatients |