Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts
Despite availability of commercially available cell lines, primary cultures of cells remain the best available natural resource for in vitro research work. However, establishing primary cultures is challenging and despite availability of many protocols for primary cell cultures reproducibility is l...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-01-01
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| Series: | Indian Journal of Rheumatology |
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| Online Access: | http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2021;volume=16;issue=3;spage=298;epage=303;aulast=Sharma |
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| _version_ | 1832544200411840512 |
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| author | Supriya Sharma Gaurav Pande Mohit Kumar Rai Vikas Agarwal |
| author_facet | Supriya Sharma Gaurav Pande Mohit Kumar Rai Vikas Agarwal |
| author_sort | Supriya Sharma |
| collection | DOAJ |
| description | Despite availability of commercially available cell lines, primary cultures of cells remain the best available natural resource for in vitro research work. However, establishing primary cultures is challenging and despite availability of many protocols for primary cell cultures reproducibility is lacking either due to cumbersome protocol, need for special reagents, and requirement of training. Herein, we present our simplistic least manipulative protocols for establishing primary human dermal fibroblasts, human pancreatic stellate cells, and human peritoneal fibroblasts that may be followed by other research laboratories for in vitro studies. |
| format | Article |
| id | doaj-art-6387a8bd94c045938be0011290227ae7 |
| institution | Kabale University |
| issn | 0973-3698 0973-3701 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Indian Journal of Rheumatology |
| spelling | doaj-art-6387a8bd94c045938be0011290227ae72025-02-03T10:51:18ZengSAGE PublishingIndian Journal of Rheumatology0973-36980973-37012021-01-0116329830310.4103/injr.injr_160_20Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblastsSupriya SharmaGaurav PandeMohit Kumar RaiVikas AgarwalDespite availability of commercially available cell lines, primary cultures of cells remain the best available natural resource for in vitro research work. However, establishing primary cultures is challenging and despite availability of many protocols for primary cell cultures reproducibility is lacking either due to cumbersome protocol, need for special reagents, and requirement of training. Herein, we present our simplistic least manipulative protocols for establishing primary human dermal fibroblasts, human pancreatic stellate cells, and human peritoneal fibroblasts that may be followed by other research laboratories for in vitro studies.http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2021;volume=16;issue=3;spage=298;epage=303;aulast=Sharmaculture protocolsdermal fibroblastsenzymatic digestionexplant culturepancreatic stellate cellsperitoneal fibroblasts |
| spellingShingle | Supriya Sharma Gaurav Pande Mohit Kumar Rai Vikas Agarwal Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts Indian Journal of Rheumatology culture protocols dermal fibroblasts enzymatic digestion explant culture pancreatic stellate cells peritoneal fibroblasts |
| title | Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts |
| title_full | Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts |
| title_fullStr | Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts |
| title_full_unstemmed | Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts |
| title_short | Indigenous primary culture protocols for human adult skin fibroblast, pancreatic stellate cells, and peritoneal fibroblasts |
| title_sort | indigenous primary culture protocols for human adult skin fibroblast pancreatic stellate cells and peritoneal fibroblasts |
| topic | culture protocols dermal fibroblasts enzymatic digestion explant culture pancreatic stellate cells peritoneal fibroblasts |
| url | http://www.indianjrheumatol.com/article.asp?issn=0973-3698;year=2021;volume=16;issue=3;spage=298;epage=303;aulast=Sharma |
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