Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration
Objective To investigate the expression of RNA m<sup>7</sup>G methyltransferase 1 (METTL1) in various types of tumors and its association with prognosis and immune infiltration, in order to provide a new reference for the screening of cancer biomarkers and their mechanisms of occurrence...
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Editorial Office of Journal of Guangxi Medical University
2024-07-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.003 |
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| author | CHENG Wenli FU Jiaqi ZHU Chenyu ZHANG Xinyu TAN Luyi ZHANG Wenjuan |
| author_facet | CHENG Wenli FU Jiaqi ZHU Chenyu ZHANG Xinyu TAN Luyi ZHANG Wenjuan |
| author_sort | CHENG Wenli |
| collection | DOAJ |
| description | Objective To investigate the expression of RNA m<sup>7</sup>G methyltransferase 1 (METTL1) in various types of tumors and its association with prognosis and immune infiltration, in order to provide a new reference for the screening of cancer biomarkers and their mechanisms of occurrence and development. Methods The Cancer Genome Atlas (TCGA) was used to analyze the mRNA expression profile of METTL1 in 33 tumors and the differences in METTL1 expression between the tumor group and the normal control group. Cox regression model and Kaplan-Meier were used to analyze the correlation between METTL1 expression and the prognosis of cancer patients. The association between METTL1 expression and immune infiltration in liver hepatocellular carcinoma (LIHC) was analyzed, and the mRNA expression levels of METTL1 and immune checkpoint in normal hepatocytes and LIHC cells were preliminatively verified by fluorescence quantitative PCR. Results The expression level of METTL1 was different between cancer tissues and adjacent tissues, and it was highly expressed in most cancer tissues. High expression of METTL1 significantly shortened the median survival time of patients with low grade glioma (LGG), LIHC and mesothelioma (MESO). The expression level of METTL1 in LIHC was positively correlated with the levels of multiple inflammatory cells. And the expression level of METTL1 was positively correlated with the expression of immune checkpoints LAG3 and PDCD1 (P＜0.001), which was consistent with the mRNA expression level in cultured LIHC cells in vitro. Conclusion METTL1 significantly promotes the occurrence and progression of most tumors, negatively regulates the prognosis survival rate of LGG, LIHC and MESO patients, and is positively correlated with immune infiltration. METTL1 is expected to be a cancer biomarker based on LAG3 and PDCD1 immunotherapy. |
| format | Article |
| id | doaj-art-6387939c6f8348ec8ff0f11c642ea302 |
| institution | DOAJ |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-07-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-6387939c6f8348ec8ff0f11c642ea3022025-08-20T02:57:28ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-07-0141795996610.16190/j.cnki.45-1211/r.2024.07.00320240703Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltrationCHENG Wenli0FU Jiaqi1ZHU Chenyu2ZHANG Xinyu3TAN Luyi4ZHANG Wenjuan5School of Medicine, Jinan University, Guangzhou 510632, ChinaSchool of Medicine, Jinan University, Guangzhou 510632, ChinaSchool of Medicine, Jinan University, Guangzhou 510632, ChinaSchool of Medicine, Jinan University, Guangzhou 510632, ChinaSchool of Medicine, Jinan University, Guangzhou 510632, ChinaSchool of Medicine, Jinan University, Guangzhou 510632, ChinaObjective To investigate the expression of RNA m<sup>7</sup>G methyltransferase 1 (METTL1) in various types of tumors and its association with prognosis and immune infiltration, in order to provide a new reference for the screening of cancer biomarkers and their mechanisms of occurrence and development. Methods The Cancer Genome Atlas (TCGA) was used to analyze the mRNA expression profile of METTL1 in 33 tumors and the differences in METTL1 expression between the tumor group and the normal control group. Cox regression model and Kaplan-Meier were used to analyze the correlation between METTL1 expression and the prognosis of cancer patients. The association between METTL1 expression and immune infiltration in liver hepatocellular carcinoma (LIHC) was analyzed, and the mRNA expression levels of METTL1 and immune checkpoint in normal hepatocytes and LIHC cells were preliminatively verified by fluorescence quantitative PCR. Results The expression level of METTL1 was different between cancer tissues and adjacent tissues, and it was highly expressed in most cancer tissues. High expression of METTL1 significantly shortened the median survival time of patients with low grade glioma (LGG), LIHC and mesothelioma (MESO). The expression level of METTL1 in LIHC was positively correlated with the levels of multiple inflammatory cells. And the expression level of METTL1 was positively correlated with the expression of immune checkpoints LAG3 and PDCD1 (P＜0.001), which was consistent with the mRNA expression level in cultured LIHC cells in vitro. Conclusion METTL1 significantly promotes the occurrence and progression of most tumors, negatively regulates the prognosis survival rate of LGG, LIHC and MESO patients, and is positively correlated with immune infiltration. METTL1 is expected to be a cancer biomarker based on LAG3 and PDCD1 immunotherapy.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.003methyltransferase 1pan-cancerimmune infiltrationbiomarkerprognosis |
| spellingShingle | CHENG Wenli FU Jiaqi ZHU Chenyu ZHANG Xinyu TAN Luyi ZHANG Wenjuan Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration Guangxi Yike Daxue xuebao methyltransferase 1 pan-cancer immune infiltration biomarker prognosis |
| title | Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration |
| title_full | Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration |
| title_fullStr | Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration |
| title_full_unstemmed | Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration |
| title_short | Preliminary analysis of association of m<sup>7</sup>G methyltransferase 1 with pan-cancer prognosis and immune infiltration |
| title_sort | preliminary analysis of association of m sup 7 sup g methyltransferase 1 with pan cancer prognosis and immune infiltration |
| topic | methyltransferase 1 pan-cancer immune infiltration biomarker prognosis |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.07.003 |
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