Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea
The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2014/594014 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849412272103882752 |
|---|---|
| author | Victor R. C. Merino Viviane Nakano Sydney M. Finegold Mario J. Avila-Campos |
| author_facet | Victor R. C. Merino Viviane Nakano Sydney M. Finegold Mario J. Avila-Campos |
| author_sort | Victor R. C. Merino |
| collection | DOAJ |
| description | The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains. |
| format | Article |
| id | doaj-art-6384c728745e472fb6c682d1ea116aee |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-6384c728745e472fb6c682d1ea116aee2025-08-20T03:34:29ZengWileyScientifica2090-908X2014-01-01201410.1155/2014/594014594014Genes Encoding Toxin of Clostridium difficile in Children with and without DiarrheaVictor R. C. Merino0Viviane Nakano1Sydney M. Finegold2Mario J. Avila-Campos3Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilAnaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilVeterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USAAnaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, BrazilThe presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.http://dx.doi.org/10.1155/2014/594014 |
| spellingShingle | Victor R. C. Merino Viviane Nakano Sydney M. Finegold Mario J. Avila-Campos Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea Scientifica |
| title | Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea |
| title_full | Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea |
| title_fullStr | Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea |
| title_full_unstemmed | Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea |
| title_short | Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea |
| title_sort | genes encoding toxin of clostridium difficile in children with and without diarrhea |
| url | http://dx.doi.org/10.1155/2014/594014 |
| work_keys_str_mv | AT victorrcmerino genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea AT vivianenakano genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea AT sydneymfinegold genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea AT mariojavilacampos genesencodingtoxinofclostridiumdifficileinchildrenwithandwithoutdiarrhea |