A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways
In this study, a strain of Wickerhamiella versatilis capable of degrading aflatoxin B1 (AFB1) was obtained. The removal conditions of AFB1 by this strain were optimized and important physiological and metabolic processes were elucidated using transcriptomics. The results single factor experiments in...
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China Food Publishing Company
2025-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-016.pdf |
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| author | WANG Lihui, PEI Yangyang, CHEN Xiaoru, ZHAO Huan, LIU Shuliang, HU Kaidi, ZHAO Ning, LI Qin, LI Jianlong |
| author_facet | WANG Lihui, PEI Yangyang, CHEN Xiaoru, ZHAO Huan, LIU Shuliang, HU Kaidi, ZHAO Ning, LI Qin, LI Jianlong |
| author_sort | WANG Lihui, PEI Yangyang, CHEN Xiaoru, ZHAO Huan, LIU Shuliang, HU Kaidi, ZHAO Ning, LI Qin, LI Jianlong |
| collection | DOAJ |
| description | In this study, a strain of Wickerhamiella versatilis capable of degrading aflatoxin B1 (AFB1) was obtained. The removal conditions of AFB1 by this strain were optimized and important physiological and metabolic processes were elucidated using transcriptomics. The results single factor experiments indicated that pH, temperature, and inoculum size had the most significant influence on the degradation rate of AFB1. Using response surface methodology (RSM), the optimal fermentation conditions that provided maximum AFB1 removal rate of 96.01% were obtained as follows: pH 5.6, 30 ℃, and inoculum size of 10%. Transcriptomic results revealed that 525 genes were differentially expressed under the stress of AFB1, including 397 up-regulated and 128 down-regulated ones. Gene annotation was particularly notable in carbon metabolism and the phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) signal pathway. Among these genes, the genes coding for lipoxygenase (LOX2S), glutathione S-transferase (GST), adenosylhomocysteinase (AHCY), aromatic amino acid aminotransferase I (ARO8), S-adenosylmethionine decarboxylase (speD) promoted the formation of AFB1-exo-8,9-epoxide-glutathione (AFBO-GSH) conjugates, disrupted the double bond of the furan ring of AFB1, and reduced its toxicity. Moreover, mass spectrometric analysis showed that in the presence of AFB1, the GSH synthesis process of this strain was accelerated, and a large amount of the intermediate substance acetyl homoserine was generated. In summary, this study has practical significance for future research on intracellular removal of AFB1. |
| format | Article |
| id | doaj-art-636fda9415db4dd28c9c96a078e9f3f2 |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-636fda9415db4dd28c9c96a078e9f3f22025-08-20T02:41:11ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461213714710.7506/spkx1002-6630-20241111-079A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation PathwaysWANG Lihui, PEI Yangyang, CHEN Xiaoru, ZHAO Huan, LIU Shuliang, HU Kaidi, ZHAO Ning, LI Qin, LI Jianlong0(1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China; 2. Beijing Products Quality Supervision and Inspection Institute, Beijing 101300, China; 3. College of Light Industry and Engineering, Sichuan Technology & Business College, Dujiangyan 611830, China; 4. Sichuan Wenjun Limited Liability Company, Chengdu 610000, China)In this study, a strain of Wickerhamiella versatilis capable of degrading aflatoxin B1 (AFB1) was obtained. The removal conditions of AFB1 by this strain were optimized and important physiological and metabolic processes were elucidated using transcriptomics. The results single factor experiments indicated that pH, temperature, and inoculum size had the most significant influence on the degradation rate of AFB1. Using response surface methodology (RSM), the optimal fermentation conditions that provided maximum AFB1 removal rate of 96.01% were obtained as follows: pH 5.6, 30 ℃, and inoculum size of 10%. Transcriptomic results revealed that 525 genes were differentially expressed under the stress of AFB1, including 397 up-regulated and 128 down-regulated ones. Gene annotation was particularly notable in carbon metabolism and the phosphatidylinositol-3-kinase-protein kinase B (PI3K/Akt) signal pathway. Among these genes, the genes coding for lipoxygenase (LOX2S), glutathione S-transferase (GST), adenosylhomocysteinase (AHCY), aromatic amino acid aminotransferase I (ARO8), S-adenosylmethionine decarboxylase (speD) promoted the formation of AFB1-exo-8,9-epoxide-glutathione (AFBO-GSH) conjugates, disrupted the double bond of the furan ring of AFB1, and reduced its toxicity. Moreover, mass spectrometric analysis showed that in the presence of AFB1, the GSH synthesis process of this strain was accelerated, and a large amount of the intermediate substance acetyl homoserine was generated. In summary, this study has practical significance for future research on intracellular removal of AFB1.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-016.pdfwickerhamiella versatilis; aflatoxins b1; intracellular enzyme; degradation mechanism; transcriptomics |
| spellingShingle | WANG Lihui, PEI Yangyang, CHEN Xiaoru, ZHAO Huan, LIU Shuliang, HU Kaidi, ZHAO Ning, LI Qin, LI Jianlong A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways Shipin Kexue wickerhamiella versatilis; aflatoxins b1; intracellular enzyme; degradation mechanism; transcriptomics |
| title | A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways |
| title_full | A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways |
| title_fullStr | A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways |
| title_full_unstemmed | A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways |
| title_short | A Strain Capable of Reducing Aflatoxin B1: Identification, Optimization of Removal Conditions and Transcriptomic Analysis of Degradation Pathways |
| title_sort | strain capable of reducing aflatoxin b1 identification optimization of removal conditions and transcriptomic analysis of degradation pathways |
| topic | wickerhamiella versatilis; aflatoxins b1; intracellular enzyme; degradation mechanism; transcriptomics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-016.pdf |
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