The effect of sample type and location on industrial workplace sink and hand dryer microbiomes

Abstract One major issue in tackling antimicrobial resistance (AMR) is the ability to effectively track resistance spread in environments where surveillance is limited. Such environments include those experiencing high volumes of hand washing and drying from multiple users. This study characterised...

Full description

Saved in:
Bibliographic Details
Main Authors: T. P. Thompson, C. J. Rice, E. Athanasakis, J. Mawhinney, B. F. Gilmore, P. Fitzgerald, T. Skvortsov, S. A. Kelly
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s12866-025-04054-9
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850242998774792192
author T. P. Thompson
C. J. Rice
E. Athanasakis
J. Mawhinney
B. F. Gilmore
P. Fitzgerald
T. Skvortsov
S. A. Kelly
author_facet T. P. Thompson
C. J. Rice
E. Athanasakis
J. Mawhinney
B. F. Gilmore
P. Fitzgerald
T. Skvortsov
S. A. Kelly
author_sort T. P. Thompson
collection DOAJ
description Abstract One major issue in tackling antimicrobial resistance (AMR) is the ability to effectively track resistance spread in environments where surveillance is limited. Such environments include those experiencing high volumes of hand washing and drying from multiple users. This study characterised the microbial populations and antimicrobial resistomes of two different sample types from a pharmaceutical industrial site as part of an AMR environmental surveillance programme. Paired samples were collected from hand dryers and adjacent sinks in distinct sampling locations: from toilets adjacent to ‘wet’ labs, and locations associated with ‘dry’ activities. Microbial populations in hand dryers were significantly different to those of sinks, whereas there was no significant difference based on sample location. The opposite effect was observed for resistomes, where profiles differed significantly based on sample location, but not sample type. When both sample type and location were considered together, differences in microbiomes were driven primarily by hand dryer profiles from different locations. Analysis of metagenomically-assembled genomes revealed the presence of many poorly characterised organisms, and suggested no specific families predominated in terms of ARG carriage. This study emphasises the impact of human activities in determining the resistome of commonly used appliances, and the need for continued AMR surveillance programmes.
format Article
id doaj-art-6366b2cf5f9945a6a85526eab3e0d326
institution OA Journals
issn 1471-2180
language English
publishDate 2025-05-01
publisher BMC
record_format Article
series BMC Microbiology
spelling doaj-art-6366b2cf5f9945a6a85526eab3e0d3262025-08-20T02:00:08ZengBMCBMC Microbiology1471-21802025-05-0125111010.1186/s12866-025-04054-9The effect of sample type and location on industrial workplace sink and hand dryer microbiomesT. P. Thompson0C. J. Rice1E. Athanasakis2J. Mawhinney3B. F. Gilmore4P. Fitzgerald5T. Skvortsov6S. A. Kelly7School of Pharmacy, Queen’s University BelfastRandox LaboratoriesRandox LaboratoriesRandox LaboratoriesSchool of Pharmacy, Queen’s University BelfastRandox LaboratoriesSchool of Pharmacy, Queen’s University BelfastSchool of Pharmacy, Queen’s University BelfastAbstract One major issue in tackling antimicrobial resistance (AMR) is the ability to effectively track resistance spread in environments where surveillance is limited. Such environments include those experiencing high volumes of hand washing and drying from multiple users. This study characterised the microbial populations and antimicrobial resistomes of two different sample types from a pharmaceutical industrial site as part of an AMR environmental surveillance programme. Paired samples were collected from hand dryers and adjacent sinks in distinct sampling locations: from toilets adjacent to ‘wet’ labs, and locations associated with ‘dry’ activities. Microbial populations in hand dryers were significantly different to those of sinks, whereas there was no significant difference based on sample location. The opposite effect was observed for resistomes, where profiles differed significantly based on sample location, but not sample type. When both sample type and location were considered together, differences in microbiomes were driven primarily by hand dryer profiles from different locations. Analysis of metagenomically-assembled genomes revealed the presence of many poorly characterised organisms, and suggested no specific families predominated in terms of ARG carriage. This study emphasises the impact of human activities in determining the resistome of commonly used appliances, and the need for continued AMR surveillance programmes.https://doi.org/10.1186/s12866-025-04054-9Antimicrobial resistanceEnvironmental surveillanceHand dryerMicrobiomeSinkWorkplace
spellingShingle T. P. Thompson
C. J. Rice
E. Athanasakis
J. Mawhinney
B. F. Gilmore
P. Fitzgerald
T. Skvortsov
S. A. Kelly
The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
BMC Microbiology
Antimicrobial resistance
Environmental surveillance
Hand dryer
Microbiome
Sink
Workplace
title The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
title_full The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
title_fullStr The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
title_full_unstemmed The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
title_short The effect of sample type and location on industrial workplace sink and hand dryer microbiomes
title_sort effect of sample type and location on industrial workplace sink and hand dryer microbiomes
topic Antimicrobial resistance
Environmental surveillance
Hand dryer
Microbiome
Sink
Workplace
url https://doi.org/10.1186/s12866-025-04054-9
work_keys_str_mv AT tpthompson theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT cjrice theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT eathanasakis theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT jmawhinney theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT bfgilmore theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT pfitzgerald theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT tskvortsov theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT sakelly theeffectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT tpthompson effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT cjrice effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT eathanasakis effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT jmawhinney effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT bfgilmore effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT pfitzgerald effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT tskvortsov effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes
AT sakelly effectofsampletypeandlocationonindustrialworkplacesinkandhanddryermicrobiomes