Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts
Background. In the pathophysiology of implant failure, metal ions and inflammation-driven osteoclasts (OC) play a crucial role. The aim of this study was to investigate whether vanadium (V) ions induce differentiation of monocytic OC precursors into osteoresorptive multinucleated cells. In addition,...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2017/9439036 |
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| author | Matthias A. König Oliver P. Gautschi Hans-Peter Simmen Luis Filgueira Dieter Cadosch |
| author_facet | Matthias A. König Oliver P. Gautschi Hans-Peter Simmen Luis Filgueira Dieter Cadosch |
| author_sort | Matthias A. König |
| collection | DOAJ |
| description | Background. In the pathophysiology of implant failure, metal ions and inflammation-driven osteoclasts (OC) play a crucial role. The aim of this study was to investigate whether vanadium (V) ions induce differentiation of monocytic OC precursors into osteoresorptive multinucleated cells. In addition, the influence of V ions on the activation and function of in vitro generated OC was observed. Methods. Human monocytes and osteoclasts were isolated from peripheral blood monocytic cells (PBMCs). Exposition with increasing concentrations (0–3 μM) of V4+/V5+ ions for 7 days followed. Assessment of OC differentiation, cell viability, and resorptional ability was performed by standard colorimetric cell viability assay 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenil)-2H-tetrazolium (MTS), tartrate-resistant acid phosphatase (TRAP) expression, and functional resorption assays on bone slides during a period of 21 days. Results. No significant differences were noted between V4+/V5+ ions (p>0.05). MTS showed significant reduction in cellular viability by V concentrations above 3 μM (p<0.05). V concentrations above 0.5 μM showed negative effects on OC activation/differentiation. Higher V concentrations showed negative effects on resorptive function (all p<0.05) without affecting cell viability. V4+/V5+ concentrations below 3 μM have negative effects on OC differentiation/function without affecting cell survival. Conclusion. Vanadium-containing implants may reduce implant failure rate by influencing osteoclast activity at the bone-implant interface. V-ligand complexes might offer new treatment options by accumulating in the bone. |
| format | Article |
| id | doaj-art-6360dfa57a9e47b88fffdf3e88b81501 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-6360dfa57a9e47b88fffdf3e88b815012025-02-03T05:51:39ZengWileyInternational Journal of Biomaterials1687-87871687-87952017-01-01201710.1155/2017/94390369439036Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human OsteoclastsMatthias A. König0Oliver P. Gautschi1Hans-Peter Simmen2Luis Filgueira3Dieter Cadosch4Department of Traumatology, University Hospital Zurich, Zurich, SwitzerlandDépartement de Neurosciences Cliniques, Geneva University Hospital, Geneva, SwitzerlandDepartment of Traumatology, University Hospital Zurich, Zurich, SwitzerlandSchool of Anatomy and Human Biology, University of Western Australia, Perth, WA, AustraliaDepartment of General and Trauma Surgery, Triemlispital, Zurich, SwitzerlandBackground. In the pathophysiology of implant failure, metal ions and inflammation-driven osteoclasts (OC) play a crucial role. The aim of this study was to investigate whether vanadium (V) ions induce differentiation of monocytic OC precursors into osteoresorptive multinucleated cells. In addition, the influence of V ions on the activation and function of in vitro generated OC was observed. Methods. Human monocytes and osteoclasts were isolated from peripheral blood monocytic cells (PBMCs). Exposition with increasing concentrations (0–3 μM) of V4+/V5+ ions for 7 days followed. Assessment of OC differentiation, cell viability, and resorptional ability was performed by standard colorimetric cell viability assay 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenil)-2H-tetrazolium (MTS), tartrate-resistant acid phosphatase (TRAP) expression, and functional resorption assays on bone slides during a period of 21 days. Results. No significant differences were noted between V4+/V5+ ions (p>0.05). MTS showed significant reduction in cellular viability by V concentrations above 3 μM (p<0.05). V concentrations above 0.5 μM showed negative effects on OC activation/differentiation. Higher V concentrations showed negative effects on resorptive function (all p<0.05) without affecting cell viability. V4+/V5+ concentrations below 3 μM have negative effects on OC differentiation/function without affecting cell survival. Conclusion. Vanadium-containing implants may reduce implant failure rate by influencing osteoclast activity at the bone-implant interface. V-ligand complexes might offer new treatment options by accumulating in the bone.http://dx.doi.org/10.1155/2017/9439036 |
| spellingShingle | Matthias A. König Oliver P. Gautschi Hans-Peter Simmen Luis Filgueira Dieter Cadosch Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts International Journal of Biomaterials |
| title | Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts |
| title_full | Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts |
| title_fullStr | Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts |
| title_full_unstemmed | Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts |
| title_short | Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts |
| title_sort | influence of vanadium 4 and 5 ions on the differentiation and activation of human osteoclasts |
| url | http://dx.doi.org/10.1155/2017/9439036 |
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