Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality
Consumer-driven demand for premium foxtail millet necessitates systematic identification of quality-determining metabolites. This investigation employed integrated metabolomic and transcriptomic analyses to compare two contrasting cultivars - the elite variety Jingu 21 (JG21) and traditional landrac...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000206 |
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| author | Yiru Zhang Xinjie Yao Pengyu Tian Canran Zhang Xiaodong Liu Hongying Li Yuanhuai Han |
| author_facet | Yiru Zhang Xinjie Yao Pengyu Tian Canran Zhang Xiaodong Liu Hongying Li Yuanhuai Han |
| author_sort | Yiru Zhang |
| collection | DOAJ |
| description | Consumer-driven demand for premium foxtail millet necessitates systematic identification of quality-determining metabolites. This investigation employed integrated metabolomic and transcriptomic analyses to compare two contrasting cultivars - the elite variety Jingu 21 (JG21) and traditional landrace Niumaobai (NMB) - during two critical grain-filling stages. 552 metabolites were identified in both cultivars, with 144 showing differential abundance. Comparative analysis of early (S2) to late (S4) grain-filling stage revealed 108 co-regulated metabolites alongside 78 JG21-exclusive and 72 NMB-specific metabolites, exhibiting differential accumulation patterns likely governing quality divergence. Differential metabolites were predominantly enriched in flavonoid and phenylpropanoid pathways between cultivars, suggesting potential roles in modulating color, nutritional quality, and grain texture. Co-expression network predictions revealed cultivar-specific regulatory associations, with 10 candidate genes potentially governing six pigmentation/nutrition-related flavonoid metabolites, while another four genes showed tentative correlations with five lignin pathway intermediates that may contribute to grain texture variations between JG21 and NMB cultivars. These findings provide mechanistic insights into metabolic determinants of millet quality while establishing a framework for targeted breeding strategies to enhance both nutritional value and sensory characteristics in foxtail millet. |
| format | Article |
| id | doaj-art-63605af2103f4c0cbab660f96fb0aa76 |
| institution | DOAJ |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-63605af2103f4c0cbab660f96fb0aa762025-08-20T02:39:38ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-011010025910.1016/j.fochms.2025.100259Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain qualityYiru Zhang0Xinjie Yao1Pengyu Tian2Canran Zhang3Xiaodong Liu4Hongying Li5Yuanhuai Han6College of Biological Sciences and Technology, Taiyuan Normal University, Yuci, Shanxi 030619, China; Houji Laboratory in Shanxi Province, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaSchool of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, ChinaCollege of Biological Sciences and Technology, Taiyuan Normal University, Yuci, Shanxi 030619, ChinaCollege of Biological Sciences and Technology, Taiyuan Normal University, Yuci, Shanxi 030619, ChinaSchool of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Houji Laboratory in Shanxi Province, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaSchool of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Houji Laboratory in Shanxi Province, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaSchool of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Houji Laboratory in Shanxi Province, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Corresponding author at: School of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaConsumer-driven demand for premium foxtail millet necessitates systematic identification of quality-determining metabolites. This investigation employed integrated metabolomic and transcriptomic analyses to compare two contrasting cultivars - the elite variety Jingu 21 (JG21) and traditional landrace Niumaobai (NMB) - during two critical grain-filling stages. 552 metabolites were identified in both cultivars, with 144 showing differential abundance. Comparative analysis of early (S2) to late (S4) grain-filling stage revealed 108 co-regulated metabolites alongside 78 JG21-exclusive and 72 NMB-specific metabolites, exhibiting differential accumulation patterns likely governing quality divergence. Differential metabolites were predominantly enriched in flavonoid and phenylpropanoid pathways between cultivars, suggesting potential roles in modulating color, nutritional quality, and grain texture. Co-expression network predictions revealed cultivar-specific regulatory associations, with 10 candidate genes potentially governing six pigmentation/nutrition-related flavonoid metabolites, while another four genes showed tentative correlations with five lignin pathway intermediates that may contribute to grain texture variations between JG21 and NMB cultivars. These findings provide mechanistic insights into metabolic determinants of millet quality while establishing a framework for targeted breeding strategies to enhance both nutritional value and sensory characteristics in foxtail millet.http://www.sciencedirect.com/science/article/pii/S2666566225000206Foxtail milletGrain qualityGrain-filling stagesMetabolomeTranscriptome |
| spellingShingle | Yiru Zhang Xinjie Yao Pengyu Tian Canran Zhang Xiaodong Liu Hongying Li Yuanhuai Han Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality Food Chemistry: Molecular Sciences Foxtail millet Grain quality Grain-filling stages Metabolome Transcriptome |
| title | Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| title_full | Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| title_fullStr | Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| title_full_unstemmed | Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| title_short | Comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| title_sort | comparative metabolome and transcriptome analysis of foxtail millet cultivars with high and low grain quality |
| topic | Foxtail millet Grain quality Grain-filling stages Metabolome Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000206 |
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