Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique

Self-emulsifying solid nanodispersion technology is emerging as an attractive strategy to prepare new eco-friendly and efficient nano-formulations due to its simple, energy efficient and easy scale-up process. However, it is still unknown whether this technology can be employed to cope with the draw...

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Main Authors: Yunfei Zhang, Xuesheng Lin, Yunlong Qian, Mingda Qin, Shujing Zhang, Lanying Wang, Yanping Luo
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Nanomaterials
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Online Access:https://www.mdpi.com/2079-4991/15/14/1056
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author Yunfei Zhang
Xuesheng Lin
Yunlong Qian
Mingda Qin
Shujing Zhang
Lanying Wang
Yanping Luo
author_facet Yunfei Zhang
Xuesheng Lin
Yunlong Qian
Mingda Qin
Shujing Zhang
Lanying Wang
Yanping Luo
author_sort Yunfei Zhang
collection DOAJ
description Self-emulsifying solid nanodispersion technology is emerging as an attractive strategy to prepare new eco-friendly and efficient nano-formulations due to its simple, energy efficient and easy scale-up process. However, it is still unknown whether this technology can be employed to cope with the drawbacks of botanical insecticides including poor water solubility, rapid photodegradation and limited targeting efficiency. In this study, rotenone (Rot) was selected as a model of botanical insecticides, and its solid nanodispersion (Rot–SND) was prepared by a self-emulsifying method combined with parameter optimization. Our target nano-formulation, consisting of 5% Rot, 20% surfactant complexes of 8% Ethylan 992 and 12% EL–80, and 75% lactose, exhibited excellent storage stability and significantly improved the pseudo-solubility of Rot by at least 250 times. The average particle size and polydispersity index (PDI) of Rot–SND were determined to be 101.19 nm and 0.21, respectively. Rot–SND displayed smaller contact angles and greater retention on both cucumber and cabbage leaves than those of a commercial emulsifiable concentrates (ECs). Rot–SND was also more resistant to photodegradation, with a degradation rate reduced by 27.01% as compared with the ECs. In addition, the toxicity of Rot–SND towards <i>Aphis gossypii</i> was 3.01 times that of the ECs, with a median lethal concentration (LC<sub>50</sub>) of 1.45 µg a.i./mL. Under the field conditions, Rot–SND showed a prolonged duration for <i>A. gossypii</i> control, with a significantly higher control efficacy (88.10%) on the 10th day than that of the ECs (77.02%). Moreover, a 2.34-fold decline in the toxicity towards nontarget mosquito larvae was observed for Rot–SND as compared with the EC. Overall, for the first time, our results indicate the role of Rot–SND as an eco-friendly and efficient way to improve the solubility, foliar affinity, photostability, bioactivity and eco-safety of Rot. This research also provided a feasible strategy to prepare more eco-friendly botanical pesticide formulations of high efficiency.
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spelling doaj-art-635947f44667493399d06c3a3ee1c2ee2025-08-20T02:47:17ZengMDPI AGNanomaterials2079-49912025-07-011514105610.3390/nano15141056Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying TechniqueYunfei Zhang0Xuesheng Lin1Yunlong Qian2Mingda Qin3Shujing Zhang4Lanying Wang5Yanping Luo6School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSelf-emulsifying solid nanodispersion technology is emerging as an attractive strategy to prepare new eco-friendly and efficient nano-formulations due to its simple, energy efficient and easy scale-up process. However, it is still unknown whether this technology can be employed to cope with the drawbacks of botanical insecticides including poor water solubility, rapid photodegradation and limited targeting efficiency. In this study, rotenone (Rot) was selected as a model of botanical insecticides, and its solid nanodispersion (Rot–SND) was prepared by a self-emulsifying method combined with parameter optimization. Our target nano-formulation, consisting of 5% Rot, 20% surfactant complexes of 8% Ethylan 992 and 12% EL–80, and 75% lactose, exhibited excellent storage stability and significantly improved the pseudo-solubility of Rot by at least 250 times. The average particle size and polydispersity index (PDI) of Rot–SND were determined to be 101.19 nm and 0.21, respectively. Rot–SND displayed smaller contact angles and greater retention on both cucumber and cabbage leaves than those of a commercial emulsifiable concentrates (ECs). Rot–SND was also more resistant to photodegradation, with a degradation rate reduced by 27.01% as compared with the ECs. In addition, the toxicity of Rot–SND towards <i>Aphis gossypii</i> was 3.01 times that of the ECs, with a median lethal concentration (LC<sub>50</sub>) of 1.45 µg a.i./mL. Under the field conditions, Rot–SND showed a prolonged duration for <i>A. gossypii</i> control, with a significantly higher control efficacy (88.10%) on the 10th day than that of the ECs (77.02%). Moreover, a 2.34-fold decline in the toxicity towards nontarget mosquito larvae was observed for Rot–SND as compared with the EC. Overall, for the first time, our results indicate the role of Rot–SND as an eco-friendly and efficient way to improve the solubility, foliar affinity, photostability, bioactivity and eco-safety of Rot. This research also provided a feasible strategy to prepare more eco-friendly botanical pesticide formulations of high efficiency.https://www.mdpi.com/2079-4991/15/14/1056solid nanodispersionself-emulsifyingrotenonephotostabilitybioactivity
spellingShingle Yunfei Zhang
Xuesheng Lin
Yunlong Qian
Mingda Qin
Shujing Zhang
Lanying Wang
Yanping Luo
Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
Nanomaterials
solid nanodispersion
self-emulsifying
rotenone
photostability
bioactivity
title Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
title_full Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
title_fullStr Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
title_full_unstemmed Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
title_short Preparation and Characterization of Efficient and Safe Rotenone Solid Nanodispersion by Self-Emulsifying Technique
title_sort preparation and characterization of efficient and safe rotenone solid nanodispersion by self emulsifying technique
topic solid nanodispersion
self-emulsifying
rotenone
photostability
bioactivity
url https://www.mdpi.com/2079-4991/15/14/1056
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AT yunlongqian preparationandcharacterizationofefficientandsaferotenonesolidnanodispersionbyselfemulsifyingtechnique
AT mingdaqin preparationandcharacterizationofefficientandsaferotenonesolidnanodispersionbyselfemulsifyingtechnique
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