Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection
The risk of children being infected with Influenza A virus (IAV) is high, and if not treated promptly, it can lead to serious illness. Compared with control group, IAV infection decreased the contents of platelet, white blood cell, lymphocyte, eosinophil, basophil, CD3+ T cells, CD4+ T cells, CD8+ T...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1478876/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582027181817856 |
|---|---|
| author | Xinyi Shi Shenghao Hua Zeyuan Chen Weiyi Cao Mengqing Xiao Wenlong Pei Zhe Cao Zhan Zhang Haibing Yang Haibing Yang Xuejun Shao Yu Xia Yu Xia |
| author_facet | Xinyi Shi Shenghao Hua Zeyuan Chen Weiyi Cao Mengqing Xiao Wenlong Pei Zhe Cao Zhan Zhang Haibing Yang Haibing Yang Xuejun Shao Yu Xia Yu Xia |
| author_sort | Xinyi Shi |
| collection | DOAJ |
| description | The risk of children being infected with Influenza A virus (IAV) is high, and if not treated promptly, it can lead to serious illness. Compared with control group, IAV infection decreased the contents of platelet, white blood cell, lymphocyte, eosinophil, basophil, CD3+ T cells, CD4+ T cells, CD8+ T cells, and B cells, while increasing the number of red blood cell. Additionally, IAV infection increased serum concentrations of total protein, albumin and lipase, while decreasing the contents of calcium, triglyceride, total bilirubin, direct bilirubin, indirect bilirubin and gamma-glutamyltransferase. However, the interactions between the respiratory microbiome and metabolites and their impact on IAV in children remains unclear. Ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) and 16S rRNA gene sequencing were employed to analysis the respiratory microbiome and serum metabolic characteristics of 85 patients with IAV infection and age-matched 55 controls with respiratory disease who tested negative for 13 types of respiratory pathogens. The serum metabolic profile of IAV patients was significantly changed, and the purine metabolism was destroyed. Purine metabolism was also enriched in H3N2 patients compared to H1N1, with increased xanthine, deoxyguanosine, and inosine. The respiratory microbiome structure in children with IAV, including H1N1 and H3N2, was significantly different from that of the control, with significantly increased Chao index. The Mantel test revealed the correlation and consistency in the trends of Haemophilus, Ureaplasma and Inosine. This study revealed the characteristics of the respiratory microbiome and serum metabolites in pediatric patients with IAV, providing a new direction for exploring the pathogenesis of IAV in children. |
| format | Article |
| id | doaj-art-63555a7c666845499a591268d61197ad |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-63555a7c666845499a591268d61197ad2025-01-30T06:22:29ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14788761478876Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infectionXinyi Shi0Shenghao Hua1Zeyuan Chen2Weiyi Cao3Mengqing Xiao4Wenlong Pei5Zhe Cao6Zhan Zhang7Haibing Yang8Haibing Yang9Xuejun Shao10Yu Xia11Yu Xia12Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaDepartment of Clinical Laboratory, Children’s Hospital of Soochow University, Suzhou, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaSCIex Analytical Instrument Trading Co., Ltd, Shanghai, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaThe First School of Clinical Medicine, Nanjing Medical University, Nanjing, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaSuzhou Center for Disease Control and Prevention, Suzhou, ChinaSuzhou College, Nanjing Medical University, Suzhou, ChinaDepartment of Clinical Laboratory, Children’s Hospital of Soochow University, Suzhou, ChinaSuzhou Center for Disease Control and Prevention, Suzhou, ChinaSuzhou College, Nanjing Medical University, Suzhou, ChinaThe risk of children being infected with Influenza A virus (IAV) is high, and if not treated promptly, it can lead to serious illness. Compared with control group, IAV infection decreased the contents of platelet, white blood cell, lymphocyte, eosinophil, basophil, CD3+ T cells, CD4+ T cells, CD8+ T cells, and B cells, while increasing the number of red blood cell. Additionally, IAV infection increased serum concentrations of total protein, albumin and lipase, while decreasing the contents of calcium, triglyceride, total bilirubin, direct bilirubin, indirect bilirubin and gamma-glutamyltransferase. However, the interactions between the respiratory microbiome and metabolites and their impact on IAV in children remains unclear. Ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) and 16S rRNA gene sequencing were employed to analysis the respiratory microbiome and serum metabolic characteristics of 85 patients with IAV infection and age-matched 55 controls with respiratory disease who tested negative for 13 types of respiratory pathogens. The serum metabolic profile of IAV patients was significantly changed, and the purine metabolism was destroyed. Purine metabolism was also enriched in H3N2 patients compared to H1N1, with increased xanthine, deoxyguanosine, and inosine. The respiratory microbiome structure in children with IAV, including H1N1 and H3N2, was significantly different from that of the control, with significantly increased Chao index. The Mantel test revealed the correlation and consistency in the trends of Haemophilus, Ureaplasma and Inosine. This study revealed the characteristics of the respiratory microbiome and serum metabolites in pediatric patients with IAV, providing a new direction for exploring the pathogenesis of IAV in children.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1478876/fullinfluenza A viruschildrenrespiratory tractmicrobiotametabolome |
| spellingShingle | Xinyi Shi Shenghao Hua Zeyuan Chen Weiyi Cao Mengqing Xiao Wenlong Pei Zhe Cao Zhan Zhang Haibing Yang Haibing Yang Xuejun Shao Yu Xia Yu Xia Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection Frontiers in Cellular and Infection Microbiology influenza A virus children respiratory tract microbiota metabolome |
| title | Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection |
| title_full | Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection |
| title_fullStr | Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection |
| title_full_unstemmed | Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection |
| title_short | Characterization of serum metabolome and respiratory microbiota in children with influenza A virus infection |
| title_sort | characterization of serum metabolome and respiratory microbiota in children with influenza a virus infection |
| topic | influenza A virus children respiratory tract microbiota metabolome |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1478876/full |
| work_keys_str_mv | AT xinyishi characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT shenghaohua characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT zeyuanchen characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT weiyicao characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT mengqingxiao characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT wenlongpei characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT zhecao characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT zhanzhang characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT haibingyang characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT haibingyang characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT xuejunshao characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT yuxia characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection AT yuxia characterizationofserummetabolomeandrespiratorymicrobiotainchildrenwithinfluenzaavirusinfection |