High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures
Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. T...
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/718754 |
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| author | Jingli Yang Songquan Wu Chenghao Li |
| author_facet | Jingli Yang Songquan Wu Chenghao Li |
| author_sort | Jingli Yang |
| collection | DOAJ |
| description | Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. Temperature strongly affected induction frequency of secondary embryogenesis. Relatively high temperature (30∘C) and germinated SEs explants were effective for induction of secondary somatic embryos, and low temperature (20∘C) was more suitable for further embryo development, plantlet conversion, and transplant survival. Somatic embryos formed on agar medium had larger cotyledons than those of embryos formed in liquid medium. Supplementing 0.1 mg L−1 6-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture and 36.5% in embryos from liquid culture. In vitro plants were successfully acclimatized in the greenhouse. The protocol established in this study will be helpful for large-scale vegetative propagation of this medicinal tree. |
| format | Article |
| id | doaj-art-6345ec23a1064ebd9e27d7e53e288e1c |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6345ec23a1064ebd9e27d7e53e288e1c2025-08-20T02:35:15ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/718754718754High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid CulturesJingli Yang0Songquan Wu1Chenghao Li2State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University), 26 Hexing Road, Harbin 150040, ChinaKey Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji, Jilin 133002, ChinaState Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University), 26 Hexing Road, Harbin 150040, ChinaEmbryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants. Well-developed SEs could be obtained through secondary somatic embryogenesis both in solid and liquid cultures. Temperature strongly affected induction frequency of secondary embryogenesis. Relatively high temperature (30∘C) and germinated SEs explants were effective for induction of secondary somatic embryos, and low temperature (20∘C) was more suitable for further embryo development, plantlet conversion, and transplant survival. Somatic embryos formed on agar medium had larger cotyledons than those of embryos formed in liquid medium. Supplementing 0.1 mg L−1 6-benzyladenine (BA) was effective for plant conversion; the rate of plant conversion was 43.3% in somatic embryos from solid culture and 36.5% in embryos from liquid culture. In vitro plants were successfully acclimatized in the greenhouse. The protocol established in this study will be helpful for large-scale vegetative propagation of this medicinal tree.http://dx.doi.org/10.1155/2013/718754 |
| spellingShingle | Jingli Yang Songquan Wu Chenghao Li High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures The Scientific World Journal |
| title | High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures |
| title_full | High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures |
| title_fullStr | High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures |
| title_full_unstemmed | High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures |
| title_short | High Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. through Solid and Liquid Cultures |
| title_sort | high efficiency secondary somatic embryogenesis in hovenia dulcis thunb through solid and liquid cultures |
| url | http://dx.doi.org/10.1155/2013/718754 |
| work_keys_str_mv | AT jingliyang highefficiencysecondarysomaticembryogenesisinhoveniadulcisthunbthroughsolidandliquidcultures AT songquanwu highefficiencysecondarysomaticembryogenesisinhoveniadulcisthunbthroughsolidandliquidcultures AT chenghaoli highefficiencysecondarysomaticembryogenesisinhoveniadulcisthunbthroughsolidandliquidcultures |