Chromosome-level genome assembly of starry flounder (Platichthys stellatus)

Abstract Starry flounder (Platichthys stellatus) is widely distributed along the coastlines of the North Pacific. As an euryhaline flatfish, it can adapt to a wide range of environmental salinity ranging from freshwater to seawater, and is a promising aquaculture flatfish species in Korea and North...

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Main Authors: Weiwei Zheng, Changlin Liu, Shenglei Han, Tengteng Wang, Tao Yang, Zhihong Liu, Dong Xu, Huizong Han, Xiaoqing Xi, Changwei Shao, Kaiqiang Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05525-4
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author Weiwei Zheng
Changlin Liu
Shenglei Han
Tengteng Wang
Tao Yang
Zhihong Liu
Dong Xu
Huizong Han
Xiaoqing Xi
Changwei Shao
Kaiqiang Liu
author_facet Weiwei Zheng
Changlin Liu
Shenglei Han
Tengteng Wang
Tao Yang
Zhihong Liu
Dong Xu
Huizong Han
Xiaoqing Xi
Changwei Shao
Kaiqiang Liu
author_sort Weiwei Zheng
collection DOAJ
description Abstract Starry flounder (Platichthys stellatus) is widely distributed along the coastlines of the North Pacific. As an euryhaline flatfish, it can adapt to a wide range of environmental salinity ranging from freshwater to seawater, and is a promising aquaculture flatfish species in Korea and North China. However, no high-quality starry flounder reference genome has been reported to date, which greatly limits the studies of genetics and functional genomics. Here, we obtained a high-quality chromosome-level starry flounder genome assembly with a length of 643.56 Mb (scaffold N50: 26.19 Mb, contig N50: 10.00 Mb) combining short-reads sequencing, PacBio HiFi sequencing, and Hi-C sequencing. Approximately 94.02% of assembled sequences were anchored into 24 pseudochromosomes, and a total of 18 telomeres were detected. Totally 22,835 protein-coding genes and 227.87 Mb repetitive sequences were identified. In summary, the high-quality chromosome-level genome assembly not only provides valuable resources for genetic research in starry flounder, but also advances the development of molecular breeding technology of starry flounder.
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publishDate 2025-07-01
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spelling doaj-art-633e4c0e46c24f36a52ec0ffa6f375d32025-08-20T03:45:45ZengNature PortfolioScientific Data2052-44632025-07-011211810.1038/s41597-025-05525-4Chromosome-level genome assembly of starry flounder (Platichthys stellatus)Weiwei Zheng0Changlin Liu1Shenglei Han2Tengteng Wang3Tao Yang4Zhihong Liu5Dong Xu6Huizong Han7Xiaoqing Xi8Changwei Shao9Kaiqiang Liu10State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesShandong Marine Resource and Environment Research InstituteState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesShandong Marine Resource and Environment Research InstituteRongcheng Marine Economic Development CenterState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesAbstract Starry flounder (Platichthys stellatus) is widely distributed along the coastlines of the North Pacific. As an euryhaline flatfish, it can adapt to a wide range of environmental salinity ranging from freshwater to seawater, and is a promising aquaculture flatfish species in Korea and North China. However, no high-quality starry flounder reference genome has been reported to date, which greatly limits the studies of genetics and functional genomics. Here, we obtained a high-quality chromosome-level starry flounder genome assembly with a length of 643.56 Mb (scaffold N50: 26.19 Mb, contig N50: 10.00 Mb) combining short-reads sequencing, PacBio HiFi sequencing, and Hi-C sequencing. Approximately 94.02% of assembled sequences were anchored into 24 pseudochromosomes, and a total of 18 telomeres were detected. Totally 22,835 protein-coding genes and 227.87 Mb repetitive sequences were identified. In summary, the high-quality chromosome-level genome assembly not only provides valuable resources for genetic research in starry flounder, but also advances the development of molecular breeding technology of starry flounder.https://doi.org/10.1038/s41597-025-05525-4
spellingShingle Weiwei Zheng
Changlin Liu
Shenglei Han
Tengteng Wang
Tao Yang
Zhihong Liu
Dong Xu
Huizong Han
Xiaoqing Xi
Changwei Shao
Kaiqiang Liu
Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
Scientific Data
title Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
title_full Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
title_fullStr Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
title_full_unstemmed Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
title_short Chromosome-level genome assembly of starry flounder (Platichthys stellatus)
title_sort chromosome level genome assembly of starry flounder platichthys stellatus
url https://doi.org/10.1038/s41597-025-05525-4
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