The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats
Cadmium, as a toxic heavy metal abounds in our habitat, and its impact on the testes contribute to the global decrease in the male fertility rate. Several natural compounds have been used to manage Cd-induced infertility successfully. Polyphenol-rich extract of Ocimum gratissimum is one plant whose...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/vmi/9165137 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850107931335327744 |
|---|---|
| author | Ikokide Emmanuel Joseph Jaja Ishmael Festus Temitayo Olabisi Ajibade Ademola Adetokunbo Oyagbemi Emmanuel Egwu Ewa Mathew Olugbenga Oyeyemi |
| author_facet | Ikokide Emmanuel Joseph Jaja Ishmael Festus Temitayo Olabisi Ajibade Ademola Adetokunbo Oyagbemi Emmanuel Egwu Ewa Mathew Olugbenga Oyeyemi |
| author_sort | Ikokide Emmanuel Joseph |
| collection | DOAJ |
| description | Cadmium, as a toxic heavy metal abounds in our habitat, and its impact on the testes contribute to the global decrease in the male fertility rate. Several natural compounds have been used to manage Cd-induced infertility successfully. Polyphenol-rich extract of Ocimum gratissimum is one plant whose potency has been reported in various studies. Nevertheless, no report has explored PREOG impairment of reprotoxicity induced by cadmium in male animals. This study, therefore, is designed to evaluate the effect of PREOG against cadmium reprotoxicity in adult male Wistar rats. 66 male rats were randomly assigned to 6 groups A to F (n = 11) per group, orally received the following treatment daily for 8 weeks: A (distilled water alone as control), B (3 mg/kg CdCL2), C (100 mg/kg PREOG), D (200 mg/kg PREOG), E (100 mg/kg PREOG + 3 mg/kg CdCL2), and F (200 mg/kg PREOG + 3 mg/kg CdCL2) at the end, six rats from each group were sacrificed, blood, semen, and testes were harvested for analysis, while the remaining males from each group were introduced to untreated female in a ratio of 2:1 for fertility study and result indicated; PREOG treatment enhances testicular weight, GSI and EPI, sperm quality and quantity, serum testosterone levels, and upregulate steroidogenic gene (3β-HSD, 17β-HSD, and StAR) expression. PREOG impaired testicular oxidative stress (enhance GST, SOD, and GPx value) and inflammation by decreasing testicular expression of COX-2 and TNF-α, and plasma IL-6 and TNF-α concentration. Also, PREOG impaired testicular apoptosis by decreasing caspase-3 protein distribution, protected histopathologic alteration of the testes, and finally enhanced reproductive outcome. The impairment of cadmium-induced reprotoxicity in male rats by PREOG treatment affirms its therapeutic and reproductive benefits. |
| format | Article |
| id | doaj-art-632765fc61574b88beaf9235fbf360f4 |
| institution | OA Journals |
| issn | 2042-0048 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-632765fc61574b88beaf9235fbf360f42025-08-20T02:38:29ZengWileyVeterinary Medicine International2042-00482025-01-01202510.1155/vmi/9165137The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male RatsIkokide Emmanuel Joseph0Jaja Ishmael Festus1Temitayo Olabisi Ajibade2Ademola Adetokunbo Oyagbemi3Emmanuel Egwu Ewa4Mathew Olugbenga Oyeyemi5Department of TheriogenologyDepartment of Livestock and Pasture ScienceDepartment of Veterinary Physiology and BiochemistryDepartment of Veterinary Physiology and BiochemistryDepartment of Veterinary Physiology and PharmacologyDepartment of TheriogenologyCadmium, as a toxic heavy metal abounds in our habitat, and its impact on the testes contribute to the global decrease in the male fertility rate. Several natural compounds have been used to manage Cd-induced infertility successfully. Polyphenol-rich extract of Ocimum gratissimum is one plant whose potency has been reported in various studies. Nevertheless, no report has explored PREOG impairment of reprotoxicity induced by cadmium in male animals. This study, therefore, is designed to evaluate the effect of PREOG against cadmium reprotoxicity in adult male Wistar rats. 66 male rats were randomly assigned to 6 groups A to F (n = 11) per group, orally received the following treatment daily for 8 weeks: A (distilled water alone as control), B (3 mg/kg CdCL2), C (100 mg/kg PREOG), D (200 mg/kg PREOG), E (100 mg/kg PREOG + 3 mg/kg CdCL2), and F (200 mg/kg PREOG + 3 mg/kg CdCL2) at the end, six rats from each group were sacrificed, blood, semen, and testes were harvested for analysis, while the remaining males from each group were introduced to untreated female in a ratio of 2:1 for fertility study and result indicated; PREOG treatment enhances testicular weight, GSI and EPI, sperm quality and quantity, serum testosterone levels, and upregulate steroidogenic gene (3β-HSD, 17β-HSD, and StAR) expression. PREOG impaired testicular oxidative stress (enhance GST, SOD, and GPx value) and inflammation by decreasing testicular expression of COX-2 and TNF-α, and plasma IL-6 and TNF-α concentration. Also, PREOG impaired testicular apoptosis by decreasing caspase-3 protein distribution, protected histopathologic alteration of the testes, and finally enhanced reproductive outcome. The impairment of cadmium-induced reprotoxicity in male rats by PREOG treatment affirms its therapeutic and reproductive benefits.http://dx.doi.org/10.1155/vmi/9165137 |
| spellingShingle | Ikokide Emmanuel Joseph Jaja Ishmael Festus Temitayo Olabisi Ajibade Ademola Adetokunbo Oyagbemi Emmanuel Egwu Ewa Mathew Olugbenga Oyeyemi The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats Veterinary Medicine International |
| title | The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats |
| title_full | The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats |
| title_fullStr | The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats |
| title_full_unstemmed | The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats |
| title_short | The Role of Steroidogenic Expression, Apoptotic, and Inflammatory Mediators in Polyphenol-Rich Extract of Ocimum gratissimum Mitigation of Cadmium-Induced Reprotoxicity in Male Rats |
| title_sort | role of steroidogenic expression apoptotic and inflammatory mediators in polyphenol rich extract of ocimum gratissimum mitigation of cadmium induced reprotoxicity in male rats |
| url | http://dx.doi.org/10.1155/vmi/9165137 |
| work_keys_str_mv | AT ikokideemmanueljoseph theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT jajaishmaelfestus theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT temitayoolabisiajibade theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT ademolaadetokunbooyagbemi theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT emmanuelegwuewa theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT mathewolugbengaoyeyemi theroleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT ikokideemmanueljoseph roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT jajaishmaelfestus roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT temitayoolabisiajibade roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT ademolaadetokunbooyagbemi roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT emmanuelegwuewa roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats AT mathewolugbengaoyeyemi roleofsteroidogenicexpressionapoptoticandinflammatorymediatorsinpolyphenolrichextractofocimumgratissimummitigationofcadmiuminducedreprotoxicityinmalerats |