A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices
ObjectiveThe aim of this study was to introduce a gelatin/bovine serum albumin (BSA) tissue standard, which provides dissolution properties identical to those of biological tissues. Further, the study evaluated whether the utilization of endodontic activating devices led to enhanced phantom dissolut...
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Korean Academy of Conservative Dentistry
2020-11-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://rde.ac/upload/pdf/rde-45-e45.pdf |
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| author | Kimia Khoshroo Brinda Shah Alexander Johnson John Baeten Katherine Barry Mohammadreza Tahriri Mohamed S. Ibrahim Lobat Tayebi |
| author_facet | Kimia Khoshroo Brinda Shah Alexander Johnson John Baeten Katherine Barry Mohammadreza Tahriri Mohamed S. Ibrahim Lobat Tayebi |
| author_sort | Kimia Khoshroo |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to introduce a gelatin/bovine serum albumin (BSA) tissue standard, which provides dissolution properties identical to those of biological tissues. Further, the study evaluated whether the utilization of endodontic activating devices led to enhanced phantom dissolution rates.Materials and MethodsBovine pulp tissue was obtained to determine a benchmark of tissue dissolution. The surface area and mass of samples were held constant while the ratio of gelatin and BSA were varied, ranging from 7.5% to 10% gelatin and 5% BSA. Each sample was placed in an individual test tube that was filled with an appropriate sodium hypochlorite solution for 1, 3, and 5 minutes, and then removed from the solution, blotted dry, and weighed again. The remaining tissue was calculated as the percent of initial tissue to determine the tissue dissolution rate. A radiopaque agent (sodium diatrizoate) and a fluorescent dye (methylene blue) were added to the phantom to allow easy quantification of phantom dissolution in a canal block model when activated using ultrasonic (EndoUltra) or sonic (EndoActivator) energy.ResultsThe 9% gelatin + 5% BSA phantom showed statistically equivalent dissolution to bovine pulp tissue at all time intervals. Furthermore, the EndoUltra yielded significantly more phantom dissolution in the canal block than the EndoActivator or syringe irrigation.ConclusionsOur phantom is comparable to biological tissue in terms of tissue dissolution and could be utilized for in vitro tests due to its injectability and detectability. |
| format | Article |
| id | doaj-art-631502417dbb4c6d814fa28baa4e00c5 |
| institution | DOAJ |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-631502417dbb4c6d814fa28baa4e00c52025-08-20T02:43:50ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662020-11-01454e4510.5395/rde.2020.45.e45929A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devicesKimia Khoshroo0Brinda Shah1Alexander Johnson2John Baeten3Katherine Barry4Mohammadreza Tahriri5Mohamed S. Ibrahim6Lobat Tayebi7Marquette University School of Dentistry, Milwaukee, WI, USAMarquette University School of Dentistry, Milwaukee, WI, USAInter Med-Vista Dental, Racine, WI, USAInter Med-Vista Dental, Racine, WI, USAInter Med-Vista Dental, Racine, WI, USAMarquette University School of Dentistry, Milwaukee, WI, USAMarquette University School of Dentistry, Milwaukee, WI, USAMarquette University School of Dentistry, Milwaukee, WI, USAObjectiveThe aim of this study was to introduce a gelatin/bovine serum albumin (BSA) tissue standard, which provides dissolution properties identical to those of biological tissues. Further, the study evaluated whether the utilization of endodontic activating devices led to enhanced phantom dissolution rates.Materials and MethodsBovine pulp tissue was obtained to determine a benchmark of tissue dissolution. The surface area and mass of samples were held constant while the ratio of gelatin and BSA were varied, ranging from 7.5% to 10% gelatin and 5% BSA. Each sample was placed in an individual test tube that was filled with an appropriate sodium hypochlorite solution for 1, 3, and 5 minutes, and then removed from the solution, blotted dry, and weighed again. The remaining tissue was calculated as the percent of initial tissue to determine the tissue dissolution rate. A radiopaque agent (sodium diatrizoate) and a fluorescent dye (methylene blue) were added to the phantom to allow easy quantification of phantom dissolution in a canal block model when activated using ultrasonic (EndoUltra) or sonic (EndoActivator) energy.ResultsThe 9% gelatin + 5% BSA phantom showed statistically equivalent dissolution to bovine pulp tissue at all time intervals. Furthermore, the EndoUltra yielded significantly more phantom dissolution in the canal block than the EndoActivator or syringe irrigation.ConclusionsOur phantom is comparable to biological tissue in terms of tissue dissolution and could be utilized for in vitro tests due to its injectability and detectability.http://rde.ac/upload/pdf/rde-45-e45.pdftissue dissolutionphantomendodontic irrigantsgelatinbovine serum albumin |
| spellingShingle | Kimia Khoshroo Brinda Shah Alexander Johnson John Baeten Katherine Barry Mohammadreza Tahriri Mohamed S. Ibrahim Lobat Tayebi A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices Restorative Dentistry & Endodontics tissue dissolution phantom endodontic irrigants gelatin bovine serum albumin |
| title | A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| title_full | A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| title_fullStr | A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| title_full_unstemmed | A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| title_short | A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| title_sort | new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices |
| topic | tissue dissolution phantom endodontic irrigants gelatin bovine serum albumin |
| url | http://rde.ac/upload/pdf/rde-45-e45.pdf |
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