Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran

Abstract Introduction Toxoplasma gondii infection is considered as one of the most important opportunistic infections and cause of death in HIV patients. Methods In this cross‐sectional study, 334 HIV positive patients were included. The molecular test was performed by the restriction fragment lengt...

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Main Authors: Maryam Nejabat, Mohammadreza Heydari, Mohammad Motamedifar, Zohre Foroozanfar, Saeid Amirizadeh Fard, Ava Hashempour, Nazani Nazari, Esmaeil Rezaei, Zahra Heydari
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Immunity, Inflammation and Disease
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Online Access:https://doi.org/10.1002/iid3.1117
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author Maryam Nejabat
Mohammadreza Heydari
Mohammad Motamedifar
Zohre Foroozanfar
Saeid Amirizadeh Fard
Ava Hashempour
Nazani Nazari
Esmaeil Rezaei
Zahra Heydari
author_facet Maryam Nejabat
Mohammadreza Heydari
Mohammad Motamedifar
Zohre Foroozanfar
Saeid Amirizadeh Fard
Ava Hashempour
Nazani Nazari
Esmaeil Rezaei
Zahra Heydari
author_sort Maryam Nejabat
collection DOAJ
description Abstract Introduction Toxoplasma gondii infection is considered as one of the most important opportunistic infections and cause of death in HIV patients. Methods In this cross‐sectional study, 334 HIV positive patients were included. The molecular test was performed by the restriction fragment length polymorphism–polymerase chain reaction method. Allelic frequency, haplotype analyses, and linkage disequilibrium were calculated. The odds ratio was calculated. The linear regression model was used to analysis of interleukin (IL)‐17A, IL‐17F, and IL‐6 single‐nucleotide polymorphism genotypes in HIV patients with and without toxoplasmosis. Results In total, 95 tested'patients (28.4%) were positive for toxoplasmosis. The risk of toxoplasma infection in the current study did not correlate with IL‐17 and IL‐6 polymorphism and the risk of contracting toxoplasma was also not significantly correlated in this study. There was no association between the frequency of alleles and the risk of toxoplasma infection in IL‐17 haplotype analysis. Conclusion The findings of this study revealed that there were significant differences in the serum levels of IL‐6 and IL‐17A, but not IL‐17F, between the case and control groups in various genetic models. However, these polymorphisms did not show a significant relationship with toxoplasma infection in HIV‐positive patients. This study represents the first investigation in Iran to explore the role of IL‐6 and IL‐17 polymorphisms in toxoplasma infection among HIV‐positive patients.
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spelling doaj-art-6311bf44ce06405c9e4a2ab08f3b00cf2025-08-20T02:35:39ZengWileyImmunity, Inflammation and Disease2050-45272024-01-01121n/an/a10.1002/iid3.1117Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern IranMaryam Nejabat0Mohammadreza Heydari1Mohammad Motamedifar2Zohre Foroozanfar3Saeid Amirizadeh Fard4Ava Hashempour5Nazani Nazari6Esmaeil Rezaei7Zahra Heydari8HIV/AIDS Research Center, Institute of Health Shiraz University of Medical Sciences Shiraz IranHIV/AIDS Research Center, Institute of Health Shiraz University of Medical Sciences Shiraz IranHIV/AIDS Research Center, Institute of Health Shiraz University of Medical Sciences Shiraz IranHIV/AIDS Research Center, Institute of Health Shiraz University of Medical Sciences Shiraz IranVirology Section, Diagnostic Laboratory Sciences and Technology Research Center School of Paramedical Sciences Shiraz IranHIV/AIDS Research Center, Institute of Health Shiraz University of Medical Sciences Shiraz IranDepartment of Immunology, Shiraz Medical School Shiraz University of Medical Sciences Shiraz IranDepartment of Biochemistry, Faculty of Biological Sciences Tarbiat Modares University Tehran IranDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology Shahid Behesti University Tehran IranAbstract Introduction Toxoplasma gondii infection is considered as one of the most important opportunistic infections and cause of death in HIV patients. Methods In this cross‐sectional study, 334 HIV positive patients were included. The molecular test was performed by the restriction fragment length polymorphism–polymerase chain reaction method. Allelic frequency, haplotype analyses, and linkage disequilibrium were calculated. The odds ratio was calculated. The linear regression model was used to analysis of interleukin (IL)‐17A, IL‐17F, and IL‐6 single‐nucleotide polymorphism genotypes in HIV patients with and without toxoplasmosis. Results In total, 95 tested'patients (28.4%) were positive for toxoplasmosis. The risk of toxoplasma infection in the current study did not correlate with IL‐17 and IL‐6 polymorphism and the risk of contracting toxoplasma was also not significantly correlated in this study. There was no association between the frequency of alleles and the risk of toxoplasma infection in IL‐17 haplotype analysis. Conclusion The findings of this study revealed that there were significant differences in the serum levels of IL‐6 and IL‐17A, but not IL‐17F, between the case and control groups in various genetic models. However, these polymorphisms did not show a significant relationship with toxoplasma infection in HIV‐positive patients. This study represents the first investigation in Iran to explore the role of IL‐6 and IL‐17 polymorphisms in toxoplasma infection among HIV‐positive patients.https://doi.org/10.1002/iid3.1117HIV/AIDS patientsIL‐17AIL‐17FIL‐6Toxoplasma gondii infection
spellingShingle Maryam Nejabat
Mohammadreza Heydari
Mohammad Motamedifar
Zohre Foroozanfar
Saeid Amirizadeh Fard
Ava Hashempour
Nazani Nazari
Esmaeil Rezaei
Zahra Heydari
Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
Immunity, Inflammation and Disease
HIV/AIDS patients
IL‐17A
IL‐17F
IL‐6
Toxoplasma gondii infection
title Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
title_full Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
title_fullStr Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
title_full_unstemmed Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
title_short Association of polymorphism of IL‐17A, IL‐17F, and IL‐6 with Toxoplasma gondii infection susceptibility in HIV/AIDS patients in Shiraz, southern Iran
title_sort association of polymorphism of il 17a il 17f and il 6 with toxoplasma gondii infection susceptibility in hiv aids patients in shiraz southern iran
topic HIV/AIDS patients
IL‐17A
IL‐17F
IL‐6
Toxoplasma gondii infection
url https://doi.org/10.1002/iid3.1117
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