Molecular response of Chironomus riparius to antibiotics
Antibiotics, like other pharmaceuticals, are continuously released into the environment as a result of human activities. Although designed to target harmful bacteria, they can also affect non-target organisms in aquatic ecosystems. Standard toxicological tests often fail to detect the subtle or long...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Current Research in Toxicology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666027X25000258 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850232285886939136 |
|---|---|
| author | Judit Kalman Yolanda Valcárcel-Rivera José Luis Martínez-Guitarte |
| author_facet | Judit Kalman Yolanda Valcárcel-Rivera José Luis Martínez-Guitarte |
| author_sort | Judit Kalman |
| collection | DOAJ |
| description | Antibiotics, like other pharmaceuticals, are continuously released into the environment as a result of human activities. Although designed to target harmful bacteria, they can also affect non-target organisms in aquatic ecosystems. Standard toxicological tests often fail to detect the subtle or long term antibiotic-induced effects, but newer methods are providing valuable insights into the molecular pathways and physiological responses they affect. Chironomus riparius, a dipteran with aquatic larvae, is widely used in toxicological testing due to its sensitivity to various toxicants. However, little is known about the molecular effects of antibiotics on this species.This study investigated the gene expression profile of C. riparius in response to antibiotics from three classes − aminoglycosides, fluoroquinolones and penicillin. Fourth instar larvae were exposed to concentrations of 0.001, 0.1 and 10 mg/L for 24 and 72 h. The expression of genes involved in hormonal regulation, detoxification, stress response and DNA repair was analysed. The results showed that all antibiotics altered mRNA levels, with three of the four (amoxicillin, neomycin and levofloxacin) downregulating genes at 24 h and upregulating them at 72 h. Genes affected by gentamicin showed the opposite trend.These transcriptional changes in response to different antibiotics highlight the complexity of the regulatory mechanisms involved in development, detoxification, stress response and DNA repair in aquatic insects. Further research is needed to better understand the molecular effects of antibiotics on this species. |
| format | Article |
| id | doaj-art-6310de52e4d4486481fb7f8d94ac6284 |
| institution | OA Journals |
| issn | 2666-027X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Toxicology |
| spelling | doaj-art-6310de52e4d4486481fb7f8d94ac62842025-08-20T02:03:14ZengElsevierCurrent Research in Toxicology2666-027X2025-01-01810023910.1016/j.crtox.2025.100239Molecular response of Chironomus riparius to antibioticsJudit Kalman0Yolanda Valcárcel-Rivera1José Luis Martínez-Guitarte2RiSAMA Group, Department of Preventive Medicine and Public Health, Faculty of Health Sciences, Rey Juan Carlos University, Madrid, Spain; Corresponding authors.RiSAMA Group, Department of Preventive Medicine and Public Health, Faculty of Health Sciences, Rey Juan Carlos University, Madrid, SpainDepartment of Mathematical Physics and Fluids, Faculty of Sciences, National Distance Education University (UNED), Madrid, Spain; Corresponding authors.Antibiotics, like other pharmaceuticals, are continuously released into the environment as a result of human activities. Although designed to target harmful bacteria, they can also affect non-target organisms in aquatic ecosystems. Standard toxicological tests often fail to detect the subtle or long term antibiotic-induced effects, but newer methods are providing valuable insights into the molecular pathways and physiological responses they affect. Chironomus riparius, a dipteran with aquatic larvae, is widely used in toxicological testing due to its sensitivity to various toxicants. However, little is known about the molecular effects of antibiotics on this species.This study investigated the gene expression profile of C. riparius in response to antibiotics from three classes − aminoglycosides, fluoroquinolones and penicillin. Fourth instar larvae were exposed to concentrations of 0.001, 0.1 and 10 mg/L for 24 and 72 h. The expression of genes involved in hormonal regulation, detoxification, stress response and DNA repair was analysed. The results showed that all antibiotics altered mRNA levels, with three of the four (amoxicillin, neomycin and levofloxacin) downregulating genes at 24 h and upregulating them at 72 h. Genes affected by gentamicin showed the opposite trend.These transcriptional changes in response to different antibiotics highlight the complexity of the regulatory mechanisms involved in development, detoxification, stress response and DNA repair in aquatic insects. Further research is needed to better understand the molecular effects of antibiotics on this species.http://www.sciencedirect.com/science/article/pii/S2666027X25000258Aquatic insectsAminoglycosidesFluoroquinolonesPenicillinGene expressions |
| spellingShingle | Judit Kalman Yolanda Valcárcel-Rivera José Luis Martínez-Guitarte Molecular response of Chironomus riparius to antibiotics Current Research in Toxicology Aquatic insects Aminoglycosides Fluoroquinolones Penicillin Gene expressions |
| title | Molecular response of Chironomus riparius to antibiotics |
| title_full | Molecular response of Chironomus riparius to antibiotics |
| title_fullStr | Molecular response of Chironomus riparius to antibiotics |
| title_full_unstemmed | Molecular response of Chironomus riparius to antibiotics |
| title_short | Molecular response of Chironomus riparius to antibiotics |
| title_sort | molecular response of chironomus riparius to antibiotics |
| topic | Aquatic insects Aminoglycosides Fluoroquinolones Penicillin Gene expressions |
| url | http://www.sciencedirect.com/science/article/pii/S2666027X25000258 |
| work_keys_str_mv | AT juditkalman molecularresponseofchironomusripariustoantibiotics AT yolandavalcarcelrivera molecularresponseofchironomusripariustoantibiotics AT joseluismartinezguitarte molecularresponseofchironomusripariustoantibiotics |