Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae

Abstract Fucoidan is a fucose-rich sulfated polysaccharide that has gained attention owing to its various biological activities. In this study, fucoidan was isolated from Saccharina japonica using an enzyme-assisted method, and its antioxidant and anti-hepatocarcinoma effects were evaluated. The fuc...

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Main Authors: Qiulin Yue, Yongxuan Liu, Fujia Li, Tao Hong, Shousen Guo, Mengrui Cai, Lin Zhao, Le Su, Song Zhang, Chen Zhao, Kunlun Li
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-94312-7
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author Qiulin Yue
Yongxuan Liu
Fujia Li
Tao Hong
Shousen Guo
Mengrui Cai
Lin Zhao
Le Su
Song Zhang
Chen Zhao
Kunlun Li
author_facet Qiulin Yue
Yongxuan Liu
Fujia Li
Tao Hong
Shousen Guo
Mengrui Cai
Lin Zhao
Le Su
Song Zhang
Chen Zhao
Kunlun Li
author_sort Qiulin Yue
collection DOAJ
description Abstract Fucoidan is a fucose-rich sulfated polysaccharide that has gained attention owing to its various biological activities. In this study, fucoidan was isolated from Saccharina japonica using an enzyme-assisted method, and its antioxidant and anti-hepatocarcinoma effects were evaluated. The fucoidan was a 112.8 kDa polysaccharide comprising seven monosaccharides: fucose, xylose, glucuronic acid, rhamnose, glucose, mannose, and galactose. The main chain residues were (1 → 3)-α-L-Fucp and (1 → 4)-α-L-Fucp units with sulfate groups at the C-2/C-4 positions of the (1 → 3)-α-L-Fucp residues. S. japonica fucoidans showed excellent antioxidant potency with values of 1.02 mg TE/g and 5.39 mg TE/g for the ABTS and FRAP assays, respectively. Additionally, they exerted antitumor efficacy and low systemic toxicity in H22 tumor-bearing mice, with a tumor inhibition rate of 42.93%. Furthermore, it significantly inhibited tumor angiogenesis and reduced pro-inflammatory cytokines levels (IL-1β, IL-6, and TNF-α). Our results suggest that fucoidan isolated from S. japonica possesses potent antioxidant and anticancer properties and may be used as a potential agent for hepatocellular carcinoma treatment.
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spelling doaj-art-62fa27d886f64ffb99d3af236fea36d22025-08-20T02:56:19ZengNature PortfolioScientific Reports2045-23222025-03-0115111410.1038/s41598-025-94312-7Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algaeQiulin Yue0Yongxuan Liu1Fujia Li2Tao Hong3Shousen Guo4Mengrui Cai5Lin Zhao6Le Su7Song Zhang8Chen Zhao9Kunlun Li10State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)Shandong Xiaoying Biotechnology Co., Ltd.College of Ocean Food and Biological Engineering, Jimei UniversityState Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences)Jinan Hangchen Biotechnology Co., Ltd.Abstract Fucoidan is a fucose-rich sulfated polysaccharide that has gained attention owing to its various biological activities. In this study, fucoidan was isolated from Saccharina japonica using an enzyme-assisted method, and its antioxidant and anti-hepatocarcinoma effects were evaluated. The fucoidan was a 112.8 kDa polysaccharide comprising seven monosaccharides: fucose, xylose, glucuronic acid, rhamnose, glucose, mannose, and galactose. The main chain residues were (1 → 3)-α-L-Fucp and (1 → 4)-α-L-Fucp units with sulfate groups at the C-2/C-4 positions of the (1 → 3)-α-L-Fucp residues. S. japonica fucoidans showed excellent antioxidant potency with values of 1.02 mg TE/g and 5.39 mg TE/g for the ABTS and FRAP assays, respectively. Additionally, they exerted antitumor efficacy and low systemic toxicity in H22 tumor-bearing mice, with a tumor inhibition rate of 42.93%. Furthermore, it significantly inhibited tumor angiogenesis and reduced pro-inflammatory cytokines levels (IL-1β, IL-6, and TNF-α). Our results suggest that fucoidan isolated from S. japonica possesses potent antioxidant and anticancer properties and may be used as a potential agent for hepatocellular carcinoma treatment.https://doi.org/10.1038/s41598-025-94312-7Saccharina JaponicaFucoidanAntioxidantAnticancerAngiogenesis
spellingShingle Qiulin Yue
Yongxuan Liu
Fujia Li
Tao Hong
Shousen Guo
Mengrui Cai
Lin Zhao
Le Su
Song Zhang
Chen Zhao
Kunlun Li
Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
Scientific Reports
Saccharina Japonica
Fucoidan
Antioxidant
Anticancer
Angiogenesis
title Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
title_full Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
title_fullStr Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
title_full_unstemmed Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
title_short Antioxidant and anticancer properties of fucoidan isolated from Saccharina Japonica brown algae
title_sort antioxidant and anticancer properties of fucoidan isolated from saccharina japonica brown algae
topic Saccharina Japonica
Fucoidan
Antioxidant
Anticancer
Angiogenesis
url https://doi.org/10.1038/s41598-025-94312-7
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