Activation of AMPK in platelets promotes the production of offspring
Platelets are terminally differentiated anucleated cells, but they still have cell-like functions and can even produce progeny platelets. However, the mechanism of platelet sprouting has not been elucidated so far. Here, we show that when platelet-rich plasma(PRP) was cultured at 37°C, platelets sho...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Platelets |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09537104.2024.2334701 |
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| author | Tong Zhang Mengnan Yang Shujun Li Rong Yan Kesheng Dai |
| author_facet | Tong Zhang Mengnan Yang Shujun Li Rong Yan Kesheng Dai |
| author_sort | Tong Zhang |
| collection | DOAJ |
| description | Platelets are terminally differentiated anucleated cells, but they still have cell-like functions and can even produce progeny platelets. However, the mechanism of platelet sprouting has not been elucidated so far. Here, we show that when platelet-rich plasma(PRP) was cultured at 37°C, platelets showed a spore phenomenon. The number of platelets increased when given a specific shear force. It is found that AMP-related signaling pathways, such as PKA and AMPK are activated in platelets in the spore state. Meanwhile, the mRNA expression levels of genes, such as CNN3, CAPZB, DBNL, KRT19, and ESPN related to PLS1 skeleton proteins also changed. Moreover, when we use the AMPK activator AICAR(AI) to treat washed platelets, cultured platelets can still appear spore phenomenon. We further demonstrate that washed platelets treated with Forskolin, an activator of PKA, not only platelet sprouting after culture but also the AMPK is activated. Taken together, these data demonstrate that AMPK plays a key role in the process of platelet budding and proliferation, suggesting a novel strategy to solve the problem of clinical platelet shortage. |
| format | Article |
| id | doaj-art-62d9ae83bbdb487585c1c82046ad6b8b |
| institution | DOAJ |
| issn | 0953-7104 1369-1635 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj-art-62d9ae83bbdb487585c1c82046ad6b8b2025-08-20T03:18:31ZengTaylor & Francis GroupPlatelets0953-71041369-16352024-12-0135110.1080/09537104.2024.2334701Activation of AMPK in platelets promotes the production of offspringTong Zhang0Mengnan Yang1Shujun Li2Rong Yan3Kesheng Dai4Suzhou Medical College, Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Soochow University, Suzhou, ChinaSuzhou Medical College, Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Soochow University, Suzhou, ChinaSuzhou Medical College, Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Soochow University, Suzhou, ChinaSuzhou Medical College, Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Soochow University, Suzhou, ChinaSuzhou Medical College, Jiangsu Institute of Hematology, Cyrus Tang Medical Institute, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, National Clinical Research Center for Hematological Diseases, Soochow University, Suzhou, ChinaPlatelets are terminally differentiated anucleated cells, but they still have cell-like functions and can even produce progeny platelets. However, the mechanism of platelet sprouting has not been elucidated so far. Here, we show that when platelet-rich plasma(PRP) was cultured at 37°C, platelets showed a spore phenomenon. The number of platelets increased when given a specific shear force. It is found that AMP-related signaling pathways, such as PKA and AMPK are activated in platelets in the spore state. Meanwhile, the mRNA expression levels of genes, such as CNN3, CAPZB, DBNL, KRT19, and ESPN related to PLS1 skeleton proteins also changed. Moreover, when we use the AMPK activator AICAR(AI) to treat washed platelets, cultured platelets can still appear spore phenomenon. We further demonstrate that washed platelets treated with Forskolin, an activator of PKA, not only platelet sprouting after culture but also the AMPK is activated. Taken together, these data demonstrate that AMPK plays a key role in the process of platelet budding and proliferation, suggesting a novel strategy to solve the problem of clinical platelet shortage.https://www.tandfonline.com/doi/10.1080/09537104.2024.2334701AMPKPKAplatelet-rich plasmaPLS1 |
| spellingShingle | Tong Zhang Mengnan Yang Shujun Li Rong Yan Kesheng Dai Activation of AMPK in platelets promotes the production of offspring Platelets AMPK PKA platelet-rich plasma PLS1 |
| title | Activation of AMPK in platelets promotes the production of offspring |
| title_full | Activation of AMPK in platelets promotes the production of offspring |
| title_fullStr | Activation of AMPK in platelets promotes the production of offspring |
| title_full_unstemmed | Activation of AMPK in platelets promotes the production of offspring |
| title_short | Activation of AMPK in platelets promotes the production of offspring |
| title_sort | activation of ampk in platelets promotes the production of offspring |
| topic | AMPK PKA platelet-rich plasma PLS1 |
| url | https://www.tandfonline.com/doi/10.1080/09537104.2024.2334701 |
| work_keys_str_mv | AT tongzhang activationofampkinplateletspromotestheproductionofoffspring AT mengnanyang activationofampkinplateletspromotestheproductionofoffspring AT shujunli activationofampkinplateletspromotestheproductionofoffspring AT rongyan activationofampkinplateletspromotestheproductionofoffspring AT keshengdai activationofampkinplateletspromotestheproductionofoffspring |