Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq.
A simple, rapid, and highly sensitive analytical method was established for identification of constituents in the spot of the thin layer chromatogram of Chuzhou Stemona sessilifolia (Miq.) Miq. (Chuzhou S. sessilifolia) with ultra performance liquid chromatography-quadrupole time-of-flight mass spec...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/3645793 |
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| author | Ya-Zhong Zhang Ye Tao Ai-Zong Shen |
| author_facet | Ya-Zhong Zhang Ye Tao Ai-Zong Shen |
| author_sort | Ya-Zhong Zhang |
| collection | DOAJ |
| description | A simple, rapid, and highly sensitive analytical method was established for identification of constituents in the spot of the thin layer chromatogram of Chuzhou Stemona sessilifolia (Miq.) Miq. (Chuzhou S. sessilifolia) with ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). The technology was applied to systematically analyze and detect the targeted spots. Compared with the fragmentation behaviors of more than thirty reference constituents, the possibly existing compounds of the target spots were identified or tentatively identified by their exact masses and diagnostic fragment ions. Finally, the four clear spots of the thin layer chromatograms of Chuzhou S. sessilifolia were screened and identified of possible molecular formula and structures. |
| format | Article |
| id | doaj-art-62d89480ec6a4633ba85d8ca2e8f3f49 |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-62d89480ec6a4633ba85d8ca2e8f3f492025-08-20T02:35:14ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/36457933645793Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq.Ya-Zhong Zhang0Ye Tao1Ai-Zong Shen2Anhui Institute of Food and Drug Control, Hefei 230051, ChinaAnhui Institute of Food and Drug Control, Hefei 230051, ChinaAnhui Provincial Hospital of the First Affiliated Hospital of University of Science and Technology of China, Hefei 230001, ChinaA simple, rapid, and highly sensitive analytical method was established for identification of constituents in the spot of the thin layer chromatogram of Chuzhou Stemona sessilifolia (Miq.) Miq. (Chuzhou S. sessilifolia) with ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). The technology was applied to systematically analyze and detect the targeted spots. Compared with the fragmentation behaviors of more than thirty reference constituents, the possibly existing compounds of the target spots were identified or tentatively identified by their exact masses and diagnostic fragment ions. Finally, the four clear spots of the thin layer chromatograms of Chuzhou S. sessilifolia were screened and identified of possible molecular formula and structures.http://dx.doi.org/10.1155/2019/3645793 |
| spellingShingle | Ya-Zhong Zhang Ye Tao Ai-Zong Shen Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. International Journal of Analytical Chemistry |
| title | Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. |
| title_full | Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. |
| title_fullStr | Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. |
| title_full_unstemmed | Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. |
| title_short | Using UPLC-QTOF-MS Method to Analyse the Spot Constituents of the Thin Layer Chromatograms for Chuzhou Stemona sessilifolia (Miq.) Miq. |
| title_sort | using uplc qtof ms method to analyse the spot constituents of the thin layer chromatograms for chuzhou stemona sessilifolia miq miq |
| url | http://dx.doi.org/10.1155/2019/3645793 |
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