Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis

Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for...

Full description

Saved in:
Bibliographic Details
Main Authors: Maxim S. Kokoulin, Yulia V. Savicheva, Alina P. Filshtein, Ludmila A. Romanenko, Marina P. Isaeva
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/23/1/29
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832588060784590848
author Maxim S. Kokoulin
Yulia V. Savicheva
Alina P. Filshtein
Ludmila A. Romanenko
Marina P. Isaeva
author_facet Maxim S. Kokoulin
Yulia V. Savicheva
Alina P. Filshtein
Ludmila A. Romanenko
Marina P. Isaeva
author_sort Maxim S. Kokoulin
collection DOAJ
description Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from the bacterium <i>Cobetia marina</i> KMM 1449, isolated from a marine sediment sample collected along the shore of the Sea of Japan. The CPS was isolated by saline solution, purified by a series of chromatographic procedures, and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The following new structure of the CPS from <i>C. marina</i> KMM 1449 was established and consisted of sulfated and simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rha<i>p</i>2S-(1→3)-β-D-Man<i>p</i>6PGro-(1→. To elucidate the genetic basis of the CPS biosynthesis, the whole genomic sequence of <i>C. marina</i> KMM 1449 was obtained. The CPS biosynthetic gene cluster (BGC) of about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha and GDP-Man), assembly (GTs genes), translocation (ABC transporter genes), sulfation (PAPS biosynthesis and sulfotransferase genes) and lipid carrier biosynthesis (<i>wcb</i> operon). Comparative analysis of the CPS BGCs from available <i>Cobetia</i> genomes showed the presence of KMM 1449-like CPS BGC among strains of all three <i>Cobetia</i> species. The study of new natural sulfated polysaccharides, as well as the elucidation of the pathways of their biosynthesis, provides the basis for the development of potential anticancer drugs.
format Article
id doaj-art-62b98aa5901d4dfdb9f9dd2be740d3bc
institution Kabale University
issn 1660-3397
language English
publishDate 2025-01-01
publisher MDPI AG
record_format Article
series Marine Drugs
spelling doaj-art-62b98aa5901d4dfdb9f9dd2be740d3bc2025-01-24T13:39:32ZengMDPI AGMarine Drugs1660-33972025-01-012312910.3390/md23010029Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its BiosynthesisMaxim S. Kokoulin0Yulia V. Savicheva1Alina P. Filshtein2Ludmila A. Romanenko3Marina P. Isaeva4G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaSome marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from the bacterium <i>Cobetia marina</i> KMM 1449, isolated from a marine sediment sample collected along the shore of the Sea of Japan. The CPS was isolated by saline solution, purified by a series of chromatographic procedures, and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The following new structure of the CPS from <i>C. marina</i> KMM 1449 was established and consisted of sulfated and simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rha<i>p</i>2S-(1→3)-β-D-Man<i>p</i>6PGro-(1→. To elucidate the genetic basis of the CPS biosynthesis, the whole genomic sequence of <i>C. marina</i> KMM 1449 was obtained. The CPS biosynthetic gene cluster (BGC) of about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha and GDP-Man), assembly (GTs genes), translocation (ABC transporter genes), sulfation (PAPS biosynthesis and sulfotransferase genes) and lipid carrier biosynthesis (<i>wcb</i> operon). Comparative analysis of the CPS BGCs from available <i>Cobetia</i> genomes showed the presence of KMM 1449-like CPS BGC among strains of all three <i>Cobetia</i> species. The study of new natural sulfated polysaccharides, as well as the elucidation of the pathways of their biosynthesis, provides the basis for the development of potential anticancer drugs.https://www.mdpi.com/1660-3397/23/1/29marine bacteria<i>Cobetia</i>sulfated capsular polysaccharideNMRgenome sequencebiosynthetic gene cluster
spellingShingle Maxim S. Kokoulin
Yulia V. Savicheva
Alina P. Filshtein
Ludmila A. Romanenko
Marina P. Isaeva
Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
Marine Drugs
marine bacteria
<i>Cobetia</i>
sulfated capsular polysaccharide
NMR
genome sequence
biosynthetic gene cluster
title Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
title_full Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
title_fullStr Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
title_full_unstemmed Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
title_short Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
title_sort structure of a sulfated capsular polysaccharide from the marine bacterium i cobetia marina i kmm 1449 and a genomic insight into its biosynthesis
topic marine bacteria
<i>Cobetia</i>
sulfated capsular polysaccharide
NMR
genome sequence
biosynthetic gene cluster
url https://www.mdpi.com/1660-3397/23/1/29
work_keys_str_mv AT maximskokoulin structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis
AT yuliavsavicheva structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis
AT alinapfilshtein structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis
AT ludmilaaromanenko structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis
AT marinapisaeva structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis