Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis
Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2025-01-01
|
Series: | Marine Drugs |
Subjects: | |
Online Access: | https://www.mdpi.com/1660-3397/23/1/29 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
_version_ | 1832588060784590848 |
---|---|
author | Maxim S. Kokoulin Yulia V. Savicheva Alina P. Filshtein Ludmila A. Romanenko Marina P. Isaeva |
author_facet | Maxim S. Kokoulin Yulia V. Savicheva Alina P. Filshtein Ludmila A. Romanenko Marina P. Isaeva |
author_sort | Maxim S. Kokoulin |
collection | DOAJ |
description | Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from the bacterium <i>Cobetia marina</i> KMM 1449, isolated from a marine sediment sample collected along the shore of the Sea of Japan. The CPS was isolated by saline solution, purified by a series of chromatographic procedures, and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The following new structure of the CPS from <i>C. marina</i> KMM 1449 was established and consisted of sulfated and simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rha<i>p</i>2S-(1→3)-β-D-Man<i>p</i>6PGro-(1→. To elucidate the genetic basis of the CPS biosynthesis, the whole genomic sequence of <i>C. marina</i> KMM 1449 was obtained. The CPS biosynthetic gene cluster (BGC) of about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha and GDP-Man), assembly (GTs genes), translocation (ABC transporter genes), sulfation (PAPS biosynthesis and sulfotransferase genes) and lipid carrier biosynthesis (<i>wcb</i> operon). Comparative analysis of the CPS BGCs from available <i>Cobetia</i> genomes showed the presence of KMM 1449-like CPS BGC among strains of all three <i>Cobetia</i> species. The study of new natural sulfated polysaccharides, as well as the elucidation of the pathways of their biosynthesis, provides the basis for the development of potential anticancer drugs. |
format | Article |
id | doaj-art-62b98aa5901d4dfdb9f9dd2be740d3bc |
institution | Kabale University |
issn | 1660-3397 |
language | English |
publishDate | 2025-01-01 |
publisher | MDPI AG |
record_format | Article |
series | Marine Drugs |
spelling | doaj-art-62b98aa5901d4dfdb9f9dd2be740d3bc2025-01-24T13:39:32ZengMDPI AGMarine Drugs1660-33972025-01-012312910.3390/md23010029Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its BiosynthesisMaxim S. Kokoulin0Yulia V. Savicheva1Alina P. Filshtein2Ludmila A. Romanenko3Marina P. Isaeva4G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, Vladivostok 690022, RussiaSome marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number of studies indicate significant biological properties of individual sulfated polysaccharides, such as antiproliferative activity, which makes them a promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from the bacterium <i>Cobetia marina</i> KMM 1449, isolated from a marine sediment sample collected along the shore of the Sea of Japan. The CPS was isolated by saline solution, purified by a series of chromatographic procedures, and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy. The following new structure of the CPS from <i>C. marina</i> KMM 1449 was established and consisted of sulfated and simultaneously phosphorylated disaccharide repeating units: →4)-α-L-Rha<i>p</i>2S-(1→3)-β-D-Man<i>p</i>6PGro-(1→. To elucidate the genetic basis of the CPS biosynthesis, the whole genomic sequence of <i>C. marina</i> KMM 1449 was obtained. The CPS biosynthetic gene cluster (BGC) of about 70 genes composes four regions encoding nucleotide sugar biosynthesis (dTDP-Rha and GDP-Man), assembly (GTs genes), translocation (ABC transporter genes), sulfation (PAPS biosynthesis and sulfotransferase genes) and lipid carrier biosynthesis (<i>wcb</i> operon). Comparative analysis of the CPS BGCs from available <i>Cobetia</i> genomes showed the presence of KMM 1449-like CPS BGC among strains of all three <i>Cobetia</i> species. The study of new natural sulfated polysaccharides, as well as the elucidation of the pathways of their biosynthesis, provides the basis for the development of potential anticancer drugs.https://www.mdpi.com/1660-3397/23/1/29marine bacteria<i>Cobetia</i>sulfated capsular polysaccharideNMRgenome sequencebiosynthetic gene cluster |
spellingShingle | Maxim S. Kokoulin Yulia V. Savicheva Alina P. Filshtein Ludmila A. Romanenko Marina P. Isaeva Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis Marine Drugs marine bacteria <i>Cobetia</i> sulfated capsular polysaccharide NMR genome sequence biosynthetic gene cluster |
title | Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis |
title_full | Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis |
title_fullStr | Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis |
title_full_unstemmed | Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis |
title_short | Structure of a Sulfated Capsular Polysaccharide from the Marine Bacterium <i>Cobetia marina</i> KMM 1449 and a Genomic Insight into Its Biosynthesis |
title_sort | structure of a sulfated capsular polysaccharide from the marine bacterium i cobetia marina i kmm 1449 and a genomic insight into its biosynthesis |
topic | marine bacteria <i>Cobetia</i> sulfated capsular polysaccharide NMR genome sequence biosynthetic gene cluster |
url | https://www.mdpi.com/1660-3397/23/1/29 |
work_keys_str_mv | AT maximskokoulin structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis AT yuliavsavicheva structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis AT alinapfilshtein structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis AT ludmilaaromanenko structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis AT marinapisaeva structureofasulfatedcapsularpolysaccharidefromthemarinebacteriumicobetiamarinaikmm1449andagenomicinsightintoitsbiosynthesis |