<i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety
<i>Salmonella</i>, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, <i>Salmonella pullorum</i...
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MDPI AG
2024-11-01
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| author | Xinxin Jin Xiuxiu Sun Qin Lu Zui Wang Zhenggang Zhang Xiaochun Ling Yunpeng Xu Ruiqin Liang Junjie Yang Li Li Tengfei Zhang Qingping Luo Guofu Cheng |
| author_facet | Xinxin Jin Xiuxiu Sun Qin Lu Zui Wang Zhenggang Zhang Xiaochun Ling Yunpeng Xu Ruiqin Liang Junjie Yang Li Li Tengfei Zhang Qingping Luo Guofu Cheng |
| author_sort | Xinxin Jin |
| collection | DOAJ |
| description | <i>Salmonella</i>, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, <i>Salmonella pullorum</i> phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples. The results revealed that phage X5 is a novel <i>Myoviridae</i> phage. Phage X5 has adequate temperature tolerance (28 °C–60 °C), pH stability (4–12), and a broad host range of <i>Salmonella</i> bacteria (87.50% of tested strains). The addition of phage X5 (MOI of 100 and 1000) to milk inoculated with <i>Salmonella</i> reduced the number of <i>Salmonella</i> by 0.72 to 0.93 log<sub>10</sub> CFU/mL and 0.66 to 1.06 log<sub>10</sub> CFU/mL at 4 °C and 25 °C, respectively. The addition of phage X5 (MOI of 100 and 1000) to chicken breast inoculated with <i>Salmonella</i> reduced bacterial numbers by 1.13 to 2.42 log<sub>10</sub> CFU/mL and 0.81 to 1.25 log<sub>10</sub> CFU/mL at 4 °C and 25 °C, respectively. Phage X5 has bactericidal activity against <i>Salmonella</i> and can be used as a potential biological bacteriostatic agent to remove mature biofilms of <i>Salmonella</i> or for the prevention and control of <i>Salmonella</i>. |
| format | Article |
| id | doaj-art-62b784e7add54371b19f319eef6f3ccd |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-62b784e7add54371b19f319eef6f3ccd2025-08-20T02:43:42ZengMDPI AGMicroorganisms2076-26072024-11-011212240010.3390/microorganisms12122400<i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food SafetyXinxin Jin0Xiuxiu Sun1Qin Lu2Zui Wang3Zhenggang Zhang4Xiaochun Ling5Yunpeng Xu6Ruiqin Liang7Junjie Yang8Li Li9Tengfei Zhang10Qingping Luo11Guofu Cheng12College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China<i>Salmonella</i>, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, <i>Salmonella pullorum</i> phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples. The results revealed that phage X5 is a novel <i>Myoviridae</i> phage. Phage X5 has adequate temperature tolerance (28 °C–60 °C), pH stability (4–12), and a broad host range of <i>Salmonella</i> bacteria (87.50% of tested strains). The addition of phage X5 (MOI of 100 and 1000) to milk inoculated with <i>Salmonella</i> reduced the number of <i>Salmonella</i> by 0.72 to 0.93 log<sub>10</sub> CFU/mL and 0.66 to 1.06 log<sub>10</sub> CFU/mL at 4 °C and 25 °C, respectively. The addition of phage X5 (MOI of 100 and 1000) to chicken breast inoculated with <i>Salmonella</i> reduced bacterial numbers by 1.13 to 2.42 log<sub>10</sub> CFU/mL and 0.81 to 1.25 log<sub>10</sub> CFU/mL at 4 °C and 25 °C, respectively. Phage X5 has bactericidal activity against <i>Salmonella</i> and can be used as a potential biological bacteriostatic agent to remove mature biofilms of <i>Salmonella</i> or for the prevention and control of <i>Salmonella</i>.https://www.mdpi.com/2076-2607/12/12/2400<i>Salmonella</i>phagebiofilmbacteriostatic agent |
| spellingShingle | Xinxin Jin Xiuxiu Sun Qin Lu Zui Wang Zhenggang Zhang Xiaochun Ling Yunpeng Xu Ruiqin Liang Junjie Yang Li Li Tengfei Zhang Qingping Luo Guofu Cheng <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety Microorganisms <i>Salmonella</i> phage biofilm bacteriostatic agent |
| title | <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety |
| title_full | <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety |
| title_fullStr | <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety |
| title_full_unstemmed | <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety |
| title_short | <i>Salmonella</i> Phage vB_SpuM_X5: A Novel Approach to Reducing <i>Salmonella</i> Biofilms with Implications for Food Safety |
| title_sort | i salmonella i phage vb spum x5 a novel approach to reducing i salmonella i biofilms with implications for food safety |
| topic | <i>Salmonella</i> phage biofilm bacteriostatic agent |
| url | https://www.mdpi.com/2076-2607/12/12/2400 |
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