Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation
BackgroundMoxibustion is a form of therapy that to warm the acupoints located skin by using dried mugwort leaves. It is widely used to treat gouty arthritis (GA). However, the mechanism of moxibustion on improving GA has not been fully revealed. In this study, we explore the mechanism of moxibustion...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2025.1433912/full |
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| author | Yufeng Xie Yufeng Xie Yun Chen Ting Qin Jun Li Zhichun Chang Yanfang Li Jianmei Zhang Mujun Liu Jianli Wang Rong Ren Ziliang Qian Jinxin Liu Min Chen |
| author_facet | Yufeng Xie Yufeng Xie Yun Chen Ting Qin Jun Li Zhichun Chang Yanfang Li Jianmei Zhang Mujun Liu Jianli Wang Rong Ren Ziliang Qian Jinxin Liu Min Chen |
| author_sort | Yufeng Xie |
| collection | DOAJ |
| description | BackgroundMoxibustion is a form of therapy that to warm the acupoints located skin by using dried mugwort leaves. It is widely used to treat gouty arthritis (GA). However, the mechanism of moxibustion on improving GA has not been fully revealed. In this study, we explore the mechanism of moxibustion on GA via metabolomics combined with traditional Chinese medicine (TCM) theory.MethodsThree days before model induction, the rats of moxibustion groups were treated with moxibustion in the ST36 and SP6, and then, a GA rat model induced by monosodium urate (MSU) was established. Biological samples, including joint synovial tissue and serum samples, were collected and measured by histopathological staining, molecular biology assays and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics.ResultsWe found that moxibustion could reduce the ankle edema induced by MSU crystals, decrease the expression of related proinflammatory genes, decrease the levels of serum IL-18 and IL-1β, and restore the metabolism of glycerol phospholipids, niacin and nicotinamide in GA model rats.ConclusionMoxibustion can regulate the metabolism of GA model rats widely to inhibit inflammation. Our research deepens our understanding of the complex mechanisms of moxibustion and promotes the application of moxibustion in the clinical practice. |
| format | Article |
| id | doaj-art-6298cf3dc47a4ba6bbdc34822a8cc116 |
| institution | DOAJ |
| issn | 2296-889X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj-art-6298cf3dc47a4ba6bbdc34822a8cc1162025-08-20T02:54:30ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2025-03-011210.3389/fmolb.2025.14339121433912Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulationYufeng Xie0Yufeng Xie1Yun Chen2Ting Qin3Jun Li4Zhichun Chang5Yanfang Li6Jianmei Zhang7Mujun Liu8Jianli Wang9Rong Ren10Ziliang Qian11Jinxin Liu12Min Chen13Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaThe Sixth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen, ChinaThe Sixth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen, ChinaThe Sixth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen, ChinaThe Sixth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen, ChinaThe Sixth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Graduate School of Peking University, Guangdong, Shenzhen, ChinaFaculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, ChinaBackgroundMoxibustion is a form of therapy that to warm the acupoints located skin by using dried mugwort leaves. It is widely used to treat gouty arthritis (GA). However, the mechanism of moxibustion on improving GA has not been fully revealed. In this study, we explore the mechanism of moxibustion on GA via metabolomics combined with traditional Chinese medicine (TCM) theory.MethodsThree days before model induction, the rats of moxibustion groups were treated with moxibustion in the ST36 and SP6, and then, a GA rat model induced by monosodium urate (MSU) was established. Biological samples, including joint synovial tissue and serum samples, were collected and measured by histopathological staining, molecular biology assays and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics.ResultsWe found that moxibustion could reduce the ankle edema induced by MSU crystals, decrease the expression of related proinflammatory genes, decrease the levels of serum IL-18 and IL-1β, and restore the metabolism of glycerol phospholipids, niacin and nicotinamide in GA model rats.ConclusionMoxibustion can regulate the metabolism of GA model rats widely to inhibit inflammation. Our research deepens our understanding of the complex mechanisms of moxibustion and promotes the application of moxibustion in the clinical practice.https://www.frontiersin.org/articles/10.3389/fmolb.2025.1433912/fullmoxibustiongouty arthritisinflammationmetabonomicsLC-MS |
| spellingShingle | Yufeng Xie Yufeng Xie Yun Chen Ting Qin Jun Li Zhichun Chang Yanfang Li Jianmei Zhang Mujun Liu Jianli Wang Rong Ren Ziliang Qian Jinxin Liu Min Chen Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation Frontiers in Molecular Biosciences moxibustion gouty arthritis inflammation metabonomics LC-MS |
| title | Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation |
| title_full | Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation |
| title_fullStr | Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation |
| title_full_unstemmed | Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation |
| title_short | Moxibustion inhibits inflammation in monosodium urate crystal-induced gouty arthritis model rats through metabolomic regulation |
| title_sort | moxibustion inhibits inflammation in monosodium urate crystal induced gouty arthritis model rats through metabolomic regulation |
| topic | moxibustion gouty arthritis inflammation metabonomics LC-MS |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2025.1433912/full |
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