RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury
To prepare to address the mechanisms of injury-induced nociceptor sensitization, we sequenced the translatome of the nociceptors of injured <i>Drosophila</i> larvae and those of uninjured larvae. Third-instar larvae expressing a green fluorescent protein (GFP)-tagged ribosomal subunit sp...
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2025-01-01
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| author | Christine M. Hale Kyle J. Beauchemin Courtney L. Brann Julie K. Moulton Ramaz Geguchadze Benjamin J. Harrison Geoffrey K. Ganter |
| author_facet | Christine M. Hale Kyle J. Beauchemin Courtney L. Brann Julie K. Moulton Ramaz Geguchadze Benjamin J. Harrison Geoffrey K. Ganter |
| author_sort | Christine M. Hale |
| collection | DOAJ |
| description | To prepare to address the mechanisms of injury-induced nociceptor sensitization, we sequenced the translatome of the nociceptors of injured <i>Drosophila</i> larvae and those of uninjured larvae. Third-instar larvae expressing a green fluorescent protein (GFP)-tagged ribosomal subunit specifically in Class 4 dendritic arborization neurons, recognized as pickpocket-expressing primary nociceptors, via the GAL4/UAS method, were injured by ultraviolet light or sham-injured. Larvae were subjected to translating ribosome affinity purification for the GFP tag and nociceptor-specific ribosome-bound RNA was sequenced. |
| format | Article |
| id | doaj-art-62938fc19ad24fffa59cc1dae8e9b7a7 |
| institution | DOAJ |
| issn | 2306-5729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-62938fc19ad24fffa59cc1dae8e9b7a72025-08-20T02:44:38ZengMDPI AGData2306-57292025-01-011021110.3390/data10020011RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to InjuryChristine M. Hale0Kyle J. Beauchemin1Courtney L. Brann2Julie K. Moulton3Ramaz Geguchadze4Benjamin J. Harrison5Geoffrey K. Ganter6College of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Osteopathic Medicine, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Osteopathic Medicine, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USACollege of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USATo prepare to address the mechanisms of injury-induced nociceptor sensitization, we sequenced the translatome of the nociceptors of injured <i>Drosophila</i> larvae and those of uninjured larvae. Third-instar larvae expressing a green fluorescent protein (GFP)-tagged ribosomal subunit specifically in Class 4 dendritic arborization neurons, recognized as pickpocket-expressing primary nociceptors, via the GAL4/UAS method, were injured by ultraviolet light or sham-injured. Larvae were subjected to translating ribosome affinity purification for the GFP tag and nociceptor-specific ribosome-bound RNA was sequenced.https://www.mdpi.com/2306-5729/10/2/11allodyniahyperalgesiaRNAseqnociceptorpainsensitization |
| spellingShingle | Christine M. Hale Kyle J. Beauchemin Courtney L. Brann Julie K. Moulton Ramaz Geguchadze Benjamin J. Harrison Geoffrey K. Ganter RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury Data allodynia hyperalgesia RNAseq nociceptor pain sensitization |
| title | RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury |
| title_full | RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury |
| title_fullStr | RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury |
| title_full_unstemmed | RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury |
| title_short | RNA Sequencing Dataset of <i>Drosophila</i> Nociceptor Translatomic Response to Injury |
| title_sort | rna sequencing dataset of i drosophila i nociceptor translatomic response to injury |
| topic | allodynia hyperalgesia RNAseq nociceptor pain sensitization |
| url | https://www.mdpi.com/2306-5729/10/2/11 |
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