The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i>
Anther culture is the most effective and not technically complex tool for obtaining double wheat haploids. The current study is devoted to the research of the influence of the genotype and concentration of the growth regulator 2.4-D on the efficiency of androgenesis in vitro of spring bread wheat. T...
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| Language: | Russian |
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Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
2022-12-01
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| Series: | Зерновое хозяйство России |
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| Online Access: | https://www.zhros.online/jour/article/view/2074 |
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| author | N. V. Petrash Е. А. Orlova I. Е. Likhenko V. V. Piskarev |
| author_facet | N. V. Petrash Е. А. Orlova I. Е. Likhenko V. V. Piskarev |
| author_sort | N. V. Petrash |
| collection | DOAJ |
| description | Anther culture is the most effective and not technically complex tool for obtaining double wheat haploids. The current study is devoted to the research of the influence of the genotype and concentration of the growth regulator 2.4-D on the efficiency of androgenesis in vitro of spring bread wheat. The purpose of the study was to estimate the efficiency of the anther culture of hybrids of the first and second generation and their parental varieties and to study its dependence on different concentrations of the growth regulator 2.4-D. There has been carried out the estimation of the traits of the F1 hybrids, the F2 population, and the parental varieties ‘Obskaya 2’ and ‘Novosibirskaya 15’ when 2.4-D was added to the induction medium at a concentration of 1 and 2 mg/l. There has been established that the F2 population and the parental variety ‘Novosibirskaya 15’ are characterized by the largest indicators. The average values were significantly surpassed by the F2 population in the traits ‘number of productive anthers per 100 anthers’ and ‘number of all regenerants per 100 anthers’ at 1 mg/l of 2.4-D. The variety ‘Novosibirskaya 15’ surpassed the average values in traits ‘number of sprouts per 100 anthers’ and ‘number of all regenerants per 100 sprouts’ at 2 mg /l of 2.4-D. The trial has shown that the best responsiveness of the anther culture for the studied genotypes was when the growth regulator 2.4-D was added to the induction medium at a concentration of 1 mg/l. The variety ‘Novosibirskaya 15’ has been characterized by good responsiveness in anther culture in vitro and could be used as a donor of valuable alleles in wheat breeding programs using DH technologies. |
| format | Article |
| id | doaj-art-6278c0732a5c4ab0bd5ff74f7ea10a32 |
| institution | DOAJ |
| issn | 2079-8725 2079-8733 |
| language | Russian |
| publishDate | 2022-12-01 |
| publisher | Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" |
| record_format | Article |
| series | Зерновое хозяйство России |
| spelling | doaj-art-6278c0732a5c4ab0bd5ff74f7ea10a322025-08-20T03:19:06ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332022-12-0106172210.31367/2079-8725-2022-83-6-17-22803The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i>N. V. Petrash0Е. А. Orlova1I. Е. Likhenko2V. V. Piskarev3SibRIPGB, a branch of the FRCIC&G SibB of the RASSibRIPGB, a branch of the FRCIC&G SibB of the RASSibRIPGB, a branch of the FRCIC&G SibB of the RASSibRIPGB, a branch of the FRCIC&G SibB of the RASAnther culture is the most effective and not technically complex tool for obtaining double wheat haploids. The current study is devoted to the research of the influence of the genotype and concentration of the growth regulator 2.4-D on the efficiency of androgenesis in vitro of spring bread wheat. The purpose of the study was to estimate the efficiency of the anther culture of hybrids of the first and second generation and their parental varieties and to study its dependence on different concentrations of the growth regulator 2.4-D. There has been carried out the estimation of the traits of the F1 hybrids, the F2 population, and the parental varieties ‘Obskaya 2’ and ‘Novosibirskaya 15’ when 2.4-D was added to the induction medium at a concentration of 1 and 2 mg/l. There has been established that the F2 population and the parental variety ‘Novosibirskaya 15’ are characterized by the largest indicators. The average values were significantly surpassed by the F2 population in the traits ‘number of productive anthers per 100 anthers’ and ‘number of all regenerants per 100 anthers’ at 1 mg/l of 2.4-D. The variety ‘Novosibirskaya 15’ surpassed the average values in traits ‘number of sprouts per 100 anthers’ and ‘number of all regenerants per 100 sprouts’ at 2 mg /l of 2.4-D. The trial has shown that the best responsiveness of the anther culture for the studied genotypes was when the growth regulator 2.4-D was added to the induction medium at a concentration of 1 mg/l. The variety ‘Novosibirskaya 15’ has been characterized by good responsiveness in anther culture in vitro and could be used as a donor of valuable alleles in wheat breeding programs using DH technologies.https://www.zhros.online/jour/article/view/2074wheat (triticum aestivum l.)androgenesis in vitroanther culturedoubled haploids (dh) |
| spellingShingle | N. V. Petrash Е. А. Orlova I. Е. Likhenko V. V. Piskarev The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> Зерновое хозяйство России wheat (triticum aestivum l.) androgenesis in vitro anther culture doubled haploids (dh) |
| title | The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> |
| title_full | The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> |
| title_fullStr | The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> |
| title_full_unstemmed | The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> |
| title_short | The study of the efficiency of anther culture <i>in vitro</i> of bread wheat varieties and hybrids <i>(Triticum aestivum L.)</i> |
| title_sort | study of the efficiency of anther culture i in vitro i of bread wheat varieties and hybrids i triticum aestivum l i |
| topic | wheat (triticum aestivum l.) androgenesis in vitro anther culture doubled haploids (dh) |
| url | https://www.zhros.online/jour/article/view/2074 |
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