The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries
Purpose. To evaluate the role of thiol status and its correlative relationship with the level of nitric oxide metabolites and vascular endothelial growth factor A (VEGF-A) in the blood plasma of patients with critical lower limb ischemia (CLI) after autovenous reconstructions of femoropopliteal segm...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian State Medical University (Tomsk)
2021-01-01
|
| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4154 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849773417498148864 |
|---|---|
| author | R. E. Kalinin Yu. V. Abalenikhina A. S. Pshennikov S. A. Vinogradov |
| author_facet | R. E. Kalinin Yu. V. Abalenikhina A. S. Pshennikov S. A. Vinogradov |
| author_sort | R. E. Kalinin |
| collection | DOAJ |
| description | Purpose. To evaluate the role of thiol status and its correlative relationship with the level of nitric oxide metabolites and vascular endothelial growth factor A (VEGF-A) in the blood plasma of patients with critical lower limb ischemia (CLI) after autovenous reconstructions of femoropopliteal segment’s arteries in the setting of venous endothelium of the arterial bed.Materials and methods. 54 patients with critical lower limb ischemia had been examined and divided into groups: synthetic prosthesis, in situ autovenous bypass procedure and reversed vein autovenous bypass procedure. Peripheral venous blood was taken on the 1st and 10th days, and in 1, 3 and 6 months after the operation. Nitric oxide metabolites level was examined with photocolorimetric method by reaction with Griess reagent on a microplate analyzer (Awareness Technology, USA). VEGF-A number estimation was done by ELISA test (PersonalLab, Italy) with the use of Human VEGF-A Platinum ELISA. The level of thiol (SH-) groups was estimated with the use of Ellman’s reagent (SERVA, Germany) on spectrophotometer (Saint-Petersburg, Russian Federation).Results. The concentration of VEGF-A and the level of SH-groups increase on the 10th day and after 1 month in the group of patients operated on using a synthetic prosthesis. The level of NO metabolites, the concentration of VEGF-A, and the content of SH-groups increase statistically significantly and then decrease to the initial values in the group of patients operated on by reversed vein autovenous bypass procedure. In the group of patients operated on by the in situ method, the level of nitric oxide metabolites increases, the concentration of VEGF-A increases on the 10th day, the level of SH-groups increases, and a positive correlation was found between the content of SHgroups and the concentration of VEGF-A. Conclusion. NO metabolites contribute to the build-up of SH groups and VEGF-A in patients operated by the “reversed vein” method, and in patients in the in situ group, the concentration of VEGF-A and the level of SH groups are not affected, which may be of clinical importance when prescribing NO donors. The revealed patterns of change in the level of recovered thiols, nitrogen oxide metabolites, VEGF-A in combination with analysis of early and late postoperative complications make it possible to conclude the advantage of autovenous reconstruction of the femoropopliteal segment due to functional adaptation of the venous endothelium compared to the group of patients, operated on by the method of “synthetic prosthesis”. Method in situ on biochemical and angiological indicators proved to be more favorable in terms of the clinical course, than the “reversed vein” method. |
| format | Article |
| id | doaj-art-626d1dec03af4d7db3f7a079bbce6d68 |
| institution | DOAJ |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-626d1dec03af4d7db3f7a079bbce6d682025-08-20T03:02:04ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842021-01-01194869310.20538/1682-0363-2020-4-86-932726The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteriesR. E. Kalinin0Yu. V. Abalenikhina1A. S. Pshennikov2S. A. Vinogradov3Ryazan State Medical UniversityRyazan State Medical UniversityRyazan State Medical UniversityRyazan State Medical UniversityPurpose. To evaluate the role of thiol status and its correlative relationship with the level of nitric oxide metabolites and vascular endothelial growth factor A (VEGF-A) in the blood plasma of patients with critical lower limb ischemia (CLI) after autovenous reconstructions of femoropopliteal segment’s arteries in the setting of venous endothelium of the arterial bed.Materials and methods. 54 patients with critical lower limb ischemia had been examined and divided into groups: synthetic prosthesis, in situ autovenous bypass procedure and reversed vein autovenous bypass procedure. Peripheral venous blood was taken on the 1st and 10th days, and in 1, 3 and 6 months after the operation. Nitric oxide metabolites level was examined with photocolorimetric method by reaction with Griess reagent on a microplate analyzer (Awareness Technology, USA). VEGF-A number estimation was done by ELISA test (PersonalLab, Italy) with the use of Human VEGF-A Platinum ELISA. The level of thiol (SH-) groups was estimated with the use of Ellman’s reagent (SERVA, Germany) on spectrophotometer (Saint-Petersburg, Russian Federation).Results. The concentration of VEGF-A and the level of SH-groups increase on the 10th day and after 1 month in the group of patients operated on using a synthetic prosthesis. The level of NO metabolites, the concentration of VEGF-A, and the content of SH-groups increase statistically significantly and then decrease to the initial values in the group of patients operated on by reversed vein autovenous bypass procedure. In the group of patients operated on by the in situ method, the level of nitric oxide metabolites increases, the concentration of VEGF-A increases on the 10th day, the level of SH-groups increases, and a positive correlation was found between the content of SHgroups and the concentration of VEGF-A. Conclusion. NO metabolites contribute to the build-up of SH groups and VEGF-A in patients operated by the “reversed vein” method, and in patients in the in situ group, the concentration of VEGF-A and the level of SH groups are not affected, which may be of clinical importance when prescribing NO donors. The revealed patterns of change in the level of recovered thiols, nitrogen oxide metabolites, VEGF-A in combination with analysis of early and late postoperative complications make it possible to conclude the advantage of autovenous reconstruction of the femoropopliteal segment due to functional adaptation of the venous endothelium compared to the group of patients, operated on by the method of “synthetic prosthesis”. Method in situ on biochemical and angiological indicators proved to be more favorable in terms of the clinical course, than the “reversed vein” method.https://bulletin.ssmu.ru/jour/article/view/4154nitric oxide metabolitesvascular endothelial growth factorprotein and nonprotein thiol groupsautovenous reconstruction of lower limbs arteriesreperfusion endothelial injury |
| spellingShingle | R. E. Kalinin Yu. V. Abalenikhina A. S. Pshennikov S. A. Vinogradov The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries Бюллетень сибирской медицины nitric oxide metabolites vascular endothelial growth factor protein and nonprotein thiol groups autovenous reconstruction of lower limbs arteries reperfusion endothelial injury |
| title | The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| title_full | The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| title_fullStr | The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| title_full_unstemmed | The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| title_short | The role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| title_sort | role of recovered thiols in venous endothel adaptation in case of autovenous reconstruction of lower limb arteries |
| topic | nitric oxide metabolites vascular endothelial growth factor protein and nonprotein thiol groups autovenous reconstruction of lower limbs arteries reperfusion endothelial injury |
| url | https://bulletin.ssmu.ru/jour/article/view/4154 |
| work_keys_str_mv | AT rekalinin theroleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT yuvabalenikhina theroleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT aspshennikov theroleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT savinogradov theroleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT rekalinin roleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT yuvabalenikhina roleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT aspshennikov roleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries AT savinogradov roleofrecoveredthiolsinvenousendotheladaptationincaseofautovenousreconstructionoflowerlimbarteries |