Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas

Endangered species are increasingly used as the target species in implementing and constructing new wildlife crossing structures (WCS). Highways in South Texas fragment the landscape and result in high road mortality for endangered species such as ocelots (Leopardus pardalis, a medium-sized spotted...

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Main Authors: Victoria Hanley, Kevin Ryer, Md Saydur Rahman, John H. Young, Richard J. Kline
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Ecology and Evolution
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fevo.2025.1487295/full
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author Victoria Hanley
Kevin Ryer
Md Saydur Rahman
Md Saydur Rahman
John H. Young
Richard J. Kline
Richard J. Kline
author_facet Victoria Hanley
Kevin Ryer
Md Saydur Rahman
Md Saydur Rahman
John H. Young
Richard J. Kline
Richard J. Kline
author_sort Victoria Hanley
collection DOAJ
description Endangered species are increasingly used as the target species in implementing and constructing new wildlife crossing structures (WCS). Highways in South Texas fragment the landscape and result in high road mortality for endangered species such as ocelots (Leopardus pardalis, a medium-sized spotted wild cat). In response, the Texas Department of Transportation constructed nine WCS on Farm-to-Market 106 (FM 106), a road which bisects Laguna Atascosa National Wildlife Refuge in Cameron County, Texas. With ocelots being rare and elusive, bobcats (Lynx rufus) are often used as a surrogate species to study felid behavior at road mitigation structures in South Texas. Bobcats have unique markings which allow them to be individually identified. Three methods of individual identification of bobcats using camera trap photos were compared: visual identification, HotSpotter, and a spatio-temporal strategy, with manual visual identification being the most effective. Using this method, 78 bobcats were identified across the 23 sites along FM 106 including: 17 residents, 52 transients, and 9 juveniles. Their interactions at road mitigation structures were further analyzed to determine the effectiveness of facilitating bobcat movement safely beneath the road. The number of bobcat individuals at a WCS per month was a significant predictor of the number of bobcat interactions (p < 0.001). Canopy cover was a significant predictor of bobcat crossings, with more crossings occurring in dense canopy cover than in mixed or open areas (p < 0.001). For marking behaviors at WCS, bobcat sex was not a significant factor (p = 0.749). However, residency status significantly affected marking behavior, with resident bobcats marking more frequently than transients (p < 0.001). The number of days between interactions at WCS decreased as the number of interactions since initial contact with a WCS increased, indicating that bobcats interacted more frequently with WCS over time. However, the rate of successful crossings did not increase. One male ocelot was observed at two WCS with dense vegetation, these were also the two crossings with the greatest frequency of bobcat activity and marking behaviors.
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spelling doaj-art-6269cd7b0eff437db3d4cb021646e3062025-08-26T05:28:14ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2025-08-011310.3389/fevo.2025.14872951487295Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South TexasVictoria Hanley0Kevin Ryer1Md Saydur Rahman2Md Saydur Rahman3John H. Young4Richard J. Kline5Richard J. Kline6School of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Earth, Environmental and Marine Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Earth, Environmental and Marine Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesDepartment of Environmental Affairs, Texas Department of Transportation, Austin, TX, United StatesSchool of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesSchool of Earth, Environmental and Marine Sciences, University of Texas Rio Grande Valley, Brownsville, TX, United StatesEndangered species are increasingly used as the target species in implementing and constructing new wildlife crossing structures (WCS). Highways in South Texas fragment the landscape and result in high road mortality for endangered species such as ocelots (Leopardus pardalis, a medium-sized spotted wild cat). In response, the Texas Department of Transportation constructed nine WCS on Farm-to-Market 106 (FM 106), a road which bisects Laguna Atascosa National Wildlife Refuge in Cameron County, Texas. With ocelots being rare and elusive, bobcats (Lynx rufus) are often used as a surrogate species to study felid behavior at road mitigation structures in South Texas. Bobcats have unique markings which allow them to be individually identified. Three methods of individual identification of bobcats using camera trap photos were compared: visual identification, HotSpotter, and a spatio-temporal strategy, with manual visual identification being the most effective. Using this method, 78 bobcats were identified across the 23 sites along FM 106 including: 17 residents, 52 transients, and 9 juveniles. Their interactions at road mitigation structures were further analyzed to determine the effectiveness of facilitating bobcat movement safely beneath the road. The number of bobcat individuals at a WCS per month was a significant predictor of the number of bobcat interactions (p < 0.001). Canopy cover was a significant predictor of bobcat crossings, with more crossings occurring in dense canopy cover than in mixed or open areas (p < 0.001). For marking behaviors at WCS, bobcat sex was not a significant factor (p = 0.749). However, residency status significantly affected marking behavior, with resident bobcats marking more frequently than transients (p < 0.001). The number of days between interactions at WCS decreased as the number of interactions since initial contact with a WCS increased, indicating that bobcats interacted more frequently with WCS over time. However, the rate of successful crossings did not increase. One male ocelot was observed at two WCS with dense vegetation, these were also the two crossings with the greatest frequency of bobcat activity and marking behaviors.https://www.frontiersin.org/articles/10.3389/fevo.2025.1487295/fullwildlife crossing structuressurrogate speciesHotSpotterspatio-temporal strategycamera trap
spellingShingle Victoria Hanley
Kevin Ryer
Md Saydur Rahman
Md Saydur Rahman
John H. Young
Richard J. Kline
Richard J. Kline
Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
Frontiers in Ecology and Evolution
wildlife crossing structures
surrogate species
HotSpotter
spatio-temporal strategy
camera trap
title Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
title_full Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
title_fullStr Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
title_full_unstemmed Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
title_short Identification, abundance, and behavior of North American bobcat (Lynx rufus) along road mitigation structures designed for endangered species in South Texas
title_sort identification abundance and behavior of north american bobcat lynx rufus along road mitigation structures designed for endangered species in south texas
topic wildlife crossing structures
surrogate species
HotSpotter
spatio-temporal strategy
camera trap
url https://www.frontiersin.org/articles/10.3389/fevo.2025.1487295/full
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