The impact of plasmid on regeneration and expression efficiencies of gfp gene in tobacco (Nicotiana tabacum L.)

Tobacco leaf explants were transformed by bacteria Agrobacterium tumefaciens (A. t.) and plasmid pBIN mgfp5-ER, which has a single copy of the green fluorescent gfp gene and A. t.-pART27 2mgfp5-ER, which has two copies of the gfp gene. Both plasmids have a built-in selection nptII gene for resistanc...

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Bibliographic Details
Main Authors: Niko PAVLIN, Zlata LUTHAR
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 2014-06-01
Series:Acta Agriculturae Slovenica
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Online Access:https://journals.uni-lj.si/aas/article/view/12615
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