CpG site methylation regulates mouse Rec8 gene promoter activity
The Rec8 gene is specifically expressed in fetal and adult gonads. Although the importance of REC8 in gametogenesis is widely acknowledged, the mechanisms underlying its germ cell-specific expression remain unclear. In this study, we utilized the mouse Rec8 gene sequence to construct a 2577 bp seque...
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The Society for Reproduction and Development
2025-04-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-077/_pdf/-char/en |
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| author | Mei RONG Na FENG Jinghuan LI Wuyun DALAI |
| author_facet | Mei RONG Na FENG Jinghuan LI Wuyun DALAI |
| author_sort | Mei RONG |
| collection | DOAJ |
| description | The Rec8 gene is specifically expressed in fetal and adult gonads. Although the importance of REC8 in gametogenesis is widely acknowledged, the mechanisms underlying its germ cell-specific expression remain unclear. In this study, we utilized the mouse Rec8 gene sequence to construct a 2577 bp sequence, which included intron 1 (180 bp), exon 1 (118 bp), and an upstream 2279 bp region. The dual-luciferase assay results showed significant differences in promoter activity between –650 bp and –385 bp and between –89 bp and –35 bp. This indicated that the core promoter region of the Rec8 gene may exist within these regions. Bisulfite sequencing PCR results showed that CpGs 10–19 were largely unmethylated in the testes but hypermethylated in other tissues. Interestingly, correlation analysis between CpG methylation status and Rec8 mRNA expression levels showed that methylation of CpGs 10 to 19 was negatively correlated with Rec8 mRNA expression levels (Pearson’s r = −0.991, P = 0.009). Furthermore, RNA-Seq data and bioinformatic analyses suggested that the specific expression of Rec8 may be linked to the presence of TATA-like sequences within its core promoter region. Overall, these findings indicate that Rec8 expression is regulated by the low methylation of CpG sites and the presence of TATA-like sequences in its core promoter. |
| format | Article |
| id | doaj-art-625a08bb4593437599bdfade3e0e68c6 |
| institution | OA Journals |
| issn | 0916-8818 1348-4400 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj-art-625a08bb4593437599bdfade3e0e68c62025-08-20T02:03:18ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002025-04-0171314515310.1262/jrd.2024-077jrdCpG site methylation regulates mouse Rec8 gene promoter activityMei RONG0Na FENG1Jinghuan LI2Wuyun DALAI3College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, Inner Mongolia, ChinaYonghe County culture and tourism Bureau, Yonghe County, Linfen City 041400, ChinaCollege of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, Inner Mongolia, ChinaCollege of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, Inner Mongolia, ChinaThe Rec8 gene is specifically expressed in fetal and adult gonads. Although the importance of REC8 in gametogenesis is widely acknowledged, the mechanisms underlying its germ cell-specific expression remain unclear. In this study, we utilized the mouse Rec8 gene sequence to construct a 2577 bp sequence, which included intron 1 (180 bp), exon 1 (118 bp), and an upstream 2279 bp region. The dual-luciferase assay results showed significant differences in promoter activity between –650 bp and –385 bp and between –89 bp and –35 bp. This indicated that the core promoter region of the Rec8 gene may exist within these regions. Bisulfite sequencing PCR results showed that CpGs 10–19 were largely unmethylated in the testes but hypermethylated in other tissues. Interestingly, correlation analysis between CpG methylation status and Rec8 mRNA expression levels showed that methylation of CpGs 10 to 19 was negatively correlated with Rec8 mRNA expression levels (Pearson’s r = −0.991, P = 0.009). Furthermore, RNA-Seq data and bioinformatic analyses suggested that the specific expression of Rec8 may be linked to the presence of TATA-like sequences within its core promoter region. Overall, these findings indicate that Rec8 expression is regulated by the low methylation of CpG sites and the presence of TATA-like sequences in its core promoter.https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-077/_pdf/-char/encohesindna methylationgene expressionpromoterrec8 |
| spellingShingle | Mei RONG Na FENG Jinghuan LI Wuyun DALAI CpG site methylation regulates mouse Rec8 gene promoter activity The Journal of Reproduction and Development cohesin dna methylation gene expression promoter rec8 |
| title | CpG site methylation regulates mouse Rec8 gene promoter activity |
| title_full | CpG site methylation regulates mouse Rec8 gene promoter activity |
| title_fullStr | CpG site methylation regulates mouse Rec8 gene promoter activity |
| title_full_unstemmed | CpG site methylation regulates mouse Rec8 gene promoter activity |
| title_short | CpG site methylation regulates mouse Rec8 gene promoter activity |
| title_sort | cpg site methylation regulates mouse rec8 gene promoter activity |
| topic | cohesin dna methylation gene expression promoter rec8 |
| url | https://www.jstage.jst.go.jp/article/jrd/71/3/71_2024-077/_pdf/-char/en |
| work_keys_str_mv | AT meirong cpgsitemethylationregulatesmouserec8genepromoteractivity AT nafeng cpgsitemethylationregulatesmouserec8genepromoteractivity AT jinghuanli cpgsitemethylationregulatesmouserec8genepromoteractivity AT wuyundalai cpgsitemethylationregulatesmouserec8genepromoteractivity |