The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia

The study was conducted in three different ecosystems: mamar (a typical local ecosystem close to water catchment areas), farm, and coastal ecosystems. In each ecosystem, soil samples were taken from the rhizosphere of five different plants to investigate the occurrence of PSM, molecularly identify t...

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Main Authors: Anthonius S. J. Adu Tae, Mahmuddin S.M. Nur, Yoke Ivonny Benggu, Lily F. Ishaq, I N. Prijo Soetedjo, Sri Widinugraheni, Muhammad Kasim
Format: Article
Language:English
Published: University of Brawijaya 2025-04-01
Series:Journal of Degraded and Mining Lands Management
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Online Access:https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17071
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author Anthonius S. J. Adu Tae
Mahmuddin S.M. Nur
Yoke Ivonny Benggu
Lily F. Ishaq
I N. Prijo Soetedjo
Sri Widinugraheni
Muhammad Kasim
author_facet Anthonius S. J. Adu Tae
Mahmuddin S.M. Nur
Yoke Ivonny Benggu
Lily F. Ishaq
I N. Prijo Soetedjo
Sri Widinugraheni
Muhammad Kasim
author_sort Anthonius S. J. Adu Tae
collection DOAJ
description The study was conducted in three different ecosystems: mamar (a typical local ecosystem close to water catchment areas), farm, and coastal ecosystems. In each ecosystem, soil samples were taken from the rhizosphere of five different plants to investigate the occurrence of PSM, molecularly identify the potential isolates, and evaluate their ability to increase soil phosphorus (P) availability and mungbean yield. The highest population of phosphate-solubilizing bacteria (PSB) was found in the mamar ecosystem. Phosphate-solubilizing fungi were only found in the farm and coastal ecosystems, with low populations, and restricted to the rhizosphere of a few plants. The number of PSB isolates was higher in the coastal ecosystem, followed by the farm and mamar ecosystems. The phosphate solubilizing index of the isolate was quite high, ranging from 210 to 300. One isolate from the five molecularly selected isolates from Kupang regency was identified as Aspergillus sp. (cassava rhizosphere origin), one isolate from the farm ecosystem (Moringa rhizosphere origin), and three isolates from the coastal ecosystem (Jatropha gossypiifolioa, Scheichera oleosa and Calotropis gigantea L. rhizosphere origin) were all identified as Pseudomonas sp. Two selected isolates from a previous study in Timor Tengah Selatan regency, collected from the mamar ecosystem (bamboo and Leuchaena leucocephala rhizosphere), were both identified as Acinetobacter baumannii. Inoculation of PSB resulted in higher available soil P compared to the uninoculated treatment. PSB inoculation also provided higher tissue P than the uninoculated treatment. PSB from coastal areas gave the highest pod weight and seed weight compared to other inoculation treatments.
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spelling doaj-art-62570f9d6b06473cb6237fe9845ffd722025-08-20T03:06:44ZengUniversity of BrawijayaJournal of Degraded and Mining Lands Management2339-076X2502-24582025-04-011237803781610.15243/jdmlm.2025.123.780317073The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, IndonesiaAnthonius S. J. Adu Tae0Mahmuddin S.M. Nur1Yoke Ivonny Benggu2https://orcid.org/0009-0000-6191-6803Lily F. Ishaq3https://orcid.org/0000-0002-6448-445XI N. Prijo Soetedjo4https://orcid.org/0009-0007-3598-8855Sri Widinugraheni5https://orcid.org/0000-0001-9775-9096Muhammad Kasim6Faculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaFaculty of Agriculture, Nusa Cendana University. Jl. Adisucipto, Penfui, Kupang 85148, East Nusa Tenggara, IndonesiaThe study was conducted in three different ecosystems: mamar (a typical local ecosystem close to water catchment areas), farm, and coastal ecosystems. In each ecosystem, soil samples were taken from the rhizosphere of five different plants to investigate the occurrence of PSM, molecularly identify the potential isolates, and evaluate their ability to increase soil phosphorus (P) availability and mungbean yield. The highest population of phosphate-solubilizing bacteria (PSB) was found in the mamar ecosystem. Phosphate-solubilizing fungi were only found in the farm and coastal ecosystems, with low populations, and restricted to the rhizosphere of a few plants. The number of PSB isolates was higher in the coastal ecosystem, followed by the farm and mamar ecosystems. The phosphate solubilizing index of the isolate was quite high, ranging from 210 to 300. One isolate from the five molecularly selected isolates from Kupang regency was identified as Aspergillus sp. (cassava rhizosphere origin), one isolate from the farm ecosystem (Moringa rhizosphere origin), and three isolates from the coastal ecosystem (Jatropha gossypiifolioa, Scheichera oleosa and Calotropis gigantea L. rhizosphere origin) were all identified as Pseudomonas sp. Two selected isolates from a previous study in Timor Tengah Selatan regency, collected from the mamar ecosystem (bamboo and Leuchaena leucocephala rhizosphere), were both identified as Acinetobacter baumannii. Inoculation of PSB resulted in higher available soil P compared to the uninoculated treatment. PSB inoculation also provided higher tissue P than the uninoculated treatment. PSB from coastal areas gave the highest pod weight and seed weight compared to other inoculation treatments.https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17071calcareous soilpsmphosphorouspotential isolate
spellingShingle Anthonius S. J. Adu Tae
Mahmuddin S.M. Nur
Yoke Ivonny Benggu
Lily F. Ishaq
I N. Prijo Soetedjo
Sri Widinugraheni
Muhammad Kasim
The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
Journal of Degraded and Mining Lands Management
calcareous soil
psm
phosphorous
potential isolate
title The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
title_full The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
title_fullStr The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
title_full_unstemmed The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
title_short The potential of phosphate solubilizing microorganisms (PSM) isolated from different ecosystems on calcareous soils in Timor Island, Indonesia
title_sort potential of phosphate solubilizing microorganisms psm isolated from different ecosystems on calcareous soils in timor island indonesia
topic calcareous soil
psm
phosphorous
potential isolate
url https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17071
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