Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p
Abstract Background The mechanism by which circRERE (hsa_circ_0006837) modulates the malignant progression of gastric cancer was investigated to identify a novel biomarker and therapeutic target for this disease. Methods Hsa_circ_0006837 expression in GC tissues and cells was detected by RT-qPCR. Se...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
|
| Series: | Hereditas |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41065-025-00449-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850268950678470656 |
|---|---|
| author | Yanxin He Yeyu Sun Yinglan Zheng Yanfang Jiang Na Li Wenjie Zhao Wanhua Ren |
| author_facet | Yanxin He Yeyu Sun Yinglan Zheng Yanfang Jiang Na Li Wenjie Zhao Wanhua Ren |
| author_sort | Yanxin He |
| collection | DOAJ |
| description | Abstract Background The mechanism by which circRERE (hsa_circ_0006837) modulates the malignant progression of gastric cancer was investigated to identify a novel biomarker and therapeutic target for this disease. Methods Hsa_circ_0006837 expression in GC tissues and cells was detected by RT-qPCR. Several data analysis methods were used to evaluate the significance of dysregulated hsa_circ_0006837 in GC. The patients were followed up for five years, and survival analysis was conducted using Kaplan-Meier curves. Cox regression was subsequently performed to analyze the risk factors for prognosis. The malignant behaviors of the cells were detected by the CCK-8 and Transwell assays. The relationship between hsa_circ_0006837 and miR-424-5p was assessed by conducting Spearman correlation analysis and verified by dual-luciferase reporter assay. Results Hsa_circ_0006837 expression decreased in patients with GC, indicating a poorer patient prognosis. In GC cells, hsa_circ_0006837 overexpression suppressed malignant behaviors. Mechanistically, miR-424-5p was identified as a target of hsa_circ_0006837. The overexpression of miR-424-5p partially counteracted the suppressive effects of upregulated hsa_circ_0006837 on the malignant behaviors of GC cells. FBXO21 was identified as a downstream gene of the hsa_circ_0006837/miR-424-5p axis. Conclusions To summarize, hsa_circ_0006837 is a biomarker for the prognosis of GC. Mechanistically, hsa_circ_0006837 overexpression can modulate the malignant behaviors of GC cells through miR-424-5p. |
| format | Article |
| id | doaj-art-6254e4967a604a1794b1596be7f00a0d |
| institution | OA Journals |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-6254e4967a604a1794b1596be7f00a0d2025-08-20T01:53:19ZengBMCHereditas1601-52232025-05-01162111210.1186/s41065-025-00449-wHsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5pYanxin He0Yeyu Sun1Yinglan Zheng2Yanfang Jiang3Na Li4Wenjie Zhao5Wanhua Ren6Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital)Department of Gastroenterology, Shandong Provincial HospitalAbstract Background The mechanism by which circRERE (hsa_circ_0006837) modulates the malignant progression of gastric cancer was investigated to identify a novel biomarker and therapeutic target for this disease. Methods Hsa_circ_0006837 expression in GC tissues and cells was detected by RT-qPCR. Several data analysis methods were used to evaluate the significance of dysregulated hsa_circ_0006837 in GC. The patients were followed up for five years, and survival analysis was conducted using Kaplan-Meier curves. Cox regression was subsequently performed to analyze the risk factors for prognosis. The malignant behaviors of the cells were detected by the CCK-8 and Transwell assays. The relationship between hsa_circ_0006837 and miR-424-5p was assessed by conducting Spearman correlation analysis and verified by dual-luciferase reporter assay. Results Hsa_circ_0006837 expression decreased in patients with GC, indicating a poorer patient prognosis. In GC cells, hsa_circ_0006837 overexpression suppressed malignant behaviors. Mechanistically, miR-424-5p was identified as a target of hsa_circ_0006837. The overexpression of miR-424-5p partially counteracted the suppressive effects of upregulated hsa_circ_0006837 on the malignant behaviors of GC cells. FBXO21 was identified as a downstream gene of the hsa_circ_0006837/miR-424-5p axis. Conclusions To summarize, hsa_circ_0006837 is a biomarker for the prognosis of GC. Mechanistically, hsa_circ_0006837 overexpression can modulate the malignant behaviors of GC cells through miR-424-5p.https://doi.org/10.1186/s41065-025-00449-wHsa_circ_0006837Gastric cancerPrognosisBiological functionmiR-424-5p |
| spellingShingle | Yanxin He Yeyu Sun Yinglan Zheng Yanfang Jiang Na Li Wenjie Zhao Wanhua Ren Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p Hereditas Hsa_circ_0006837 Gastric cancer Prognosis Biological function miR-424-5p |
| title | Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p |
| title_full | Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p |
| title_fullStr | Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p |
| title_full_unstemmed | Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p |
| title_short | Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p |
| title_sort | hsa circ 0006837 suppresses gastric cancer cell proliferation migration and invasion via the modulation of mir 424 5p |
| topic | Hsa_circ_0006837 Gastric cancer Prognosis Biological function miR-424-5p |
| url | https://doi.org/10.1186/s41065-025-00449-w |
| work_keys_str_mv | AT yanxinhe hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT yeyusun hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT yinglanzheng hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT yanfangjiang hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT nali hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT wenjiezhao hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p AT wanhuaren hsacirc0006837suppressesgastriccancercellproliferationmigrationandinvasionviathemodulationofmir4245p |