Genome-Wide Identification of <i>G3BP</i> Family in U’s Triangle <i>Brassica</i> Species and Analysis of Its Expression in <i>B. napus</i>

Genome-Wide Identification of <i>G3BP</i> Family in U’s Triangle <i>Brassica</i> Species and Analysis of Its Expression in <i>B. napus</i>

The RasGAP SH3 domain binding protein (G3BP) is a highly conserved family of proteins in eukaryotic organisms that coordinates signal transduction and post-transcriptional gene regulation and functions in the formation of stress granules. G3BPs have important roles in abiotic/biotic stresses in mamm...

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Main Authors: Alain Tseke Inkabanga, Qiheng Zhang, Shanshan Wang, Yanni Li, Jingyi Chen, Li Huang, Xiang Li, Zihan Deng, Xiao Yang, Mengxin Luo, Lingxia Peng, Keran Ren, Yourong Chai, Yufei Xue
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Plants
Subjects:
G3BP
<i>Brassica napus</i>
seed quality traits
abiotic/biotic stresses
<i>Brassica</i> species
genome-wide analysis
Online Access:https://www.mdpi.com/2223-7747/14/14/2247
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Summary:The RasGAP SH3 domain binding protein (G3BP) is a highly conserved family of proteins in eukaryotic organisms that coordinates signal transduction and post-transcriptional gene regulation and functions in the formation of stress granules. G3BPs have important roles in abiotic/biotic stresses in mammals, and recent research suggests that they have similar functions in higher plants. <i>Brassica</i> contains many important oilseeds, vegetables, and ornamental plants, but there are no reports on the <i>G3BP</i> family in <i>Brassica</i> species. In this study, we identified <i>G3BP</i> family genes from six species of the U’s triangle (<i>B. rapa</i>, <i>B. oleracea</i>, <i>B. nigra</i>, <i>B. napus</i>, <i>B. juncea</i>, and <i>B. carinata</i>) at the genome-wide level. We then analyzed their gene structure, protein motifs, gene duplication type, phylogeny, subcellular localization, SSR loci, and upstream miRNAs. Based on transcriptome data, we analyzed the expression patterns of <i>B. napus G3BP</i> (<i>BnaG3BP</i>) genes in various tissues/organs in response to <i>Sclerotinia</i> disease, blackleg disease, powdery mildew, dehydration, drought, heat, cold, and ABA treatments, and its involvement in seed traits including germination, α-linolenic acid content, oil content, and yellow seed. Several <i>BnaG3BP</i> DEGs might be regulated by BnaTT1. The qRT-PCR assay validated the inducibility of two cold-responsive <i>BnaG3BP</i> DEGs. This study will enrich the systematic understanding of <i>Brassica G3BP</i> family genes and lay a molecular basis for the application of <i>BnaG3BP</i> genes in stress tolerance, disease resistance, and quality improvement in rapeseed.
ISSN:2223-7747

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