Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs
Introduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins. Methodology: Eight serum samples from the o...
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The Journal of Infection in Developing Countries
2020-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12711 |
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| author | Oswaldo M Torres-Chable Ligia G Brito-Argaez Ignacio R Islas-Flores Claudia V Zaragoza-Vera Maritza Zaragoza-Vera Guadalupe Arjona-Jimenez Carlos M Baak-Baak Nohemi Cigarroa-Toledo Roberto Gonzalez-Garduño Carlos I Machain-Williams Julian E Garcia-Rejon |
| author_facet | Oswaldo M Torres-Chable Ligia G Brito-Argaez Ignacio R Islas-Flores Claudia V Zaragoza-Vera Maritza Zaragoza-Vera Guadalupe Arjona-Jimenez Carlos M Baak-Baak Nohemi Cigarroa-Toledo Roberto Gonzalez-Garduño Carlos I Machain-Williams Julian E Garcia-Rejon |
| author_sort | Oswaldo M Torres-Chable |
| collection | DOAJ |
| description |
Introduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins.
Methodology: Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS).
Results: The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples.
Conclusions: These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection.
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| format | Article |
| id | doaj-art-6233eebe8e2b4196ac4c173754d25d9d |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-6233eebe8e2b4196ac4c173754d25d9d2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-12-01141210.3855/jidc.12711Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogsOswaldo M Torres-Chable0Ligia G Brito-Argaez1Ignacio R Islas-Flores2Claudia V Zaragoza-Vera3Maritza Zaragoza-Vera4Guadalupe Arjona-Jimenez5Carlos M Baak-Baak6Nohemi Cigarroa-Toledo7Roberto Gonzalez-Garduño8Carlos I Machain-Williams9Julian E Garcia-Rejon10División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, MéxicoLaboratorio de Bioquímica y Biología Molecular, Centro de Investigaciones Científicas de Yucatán (CICY), Mérida, Yucatán, MéxicoLaboratorio de Bioquímica y Biología Molecular, Centro de Investigaciones Científicas de Yucatán (CICY), Mérida, Yucatán, MéxicoDivisión Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, MéxicoDivisión Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, MéxicoDivisión Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, TabascoLaboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, MéxicoLaboratorio de Biología Celular, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, MéxicoUnidad Regional Universitaria Sursureste, Universidad Autónoma Chapingo, Teapa, Tabasco, MéxicoLaboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, MéxicoLaboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México Introduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins. Methodology: Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS). Results: The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples. Conclusions: These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection. https://jidc.org/index.php/journal/article/view/12711Dirofilariafilariasiszoonoticimmunoreactivitytropical diseases |
| spellingShingle | Oswaldo M Torres-Chable Ligia G Brito-Argaez Ignacio R Islas-Flores Claudia V Zaragoza-Vera Maritza Zaragoza-Vera Guadalupe Arjona-Jimenez Carlos M Baak-Baak Nohemi Cigarroa-Toledo Roberto Gonzalez-Garduño Carlos I Machain-Williams Julian E Garcia-Rejon Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs Journal of Infection in Developing Countries Dirofilaria filariasis zoonotic immunoreactivity tropical diseases |
| title | Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| title_full | Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| title_fullStr | Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| title_full_unstemmed | Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| title_short | Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| title_sort | dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs |
| topic | Dirofilaria filariasis zoonotic immunoreactivity tropical diseases |
| url | https://jidc.org/index.php/journal/article/view/12711 |
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