On the localization of xylanolytic enzymes in Prevotella Bryantii B14
Prevotella bryantii B14 is a strictly anaerobic, Gram-negative, polysaccharides-degrading ruminal bacterium. EDTA/sphaeroplasting and osmotic shock procedure were used in present work to localise endoxylanolytic, b -xylosidase and a -L-arabinofuranosidase activities of P. bryantii B14. Late exponen...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1997-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/16053 |
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| author | Romana MARINŠEK-LOGAR Franc Viktor NEKREP |
| author_facet | Romana MARINŠEK-LOGAR Franc Viktor NEKREP |
| author_sort | Romana MARINŠEK-LOGAR |
| collection | DOAJ |
| description |
Prevotella bryantii B14 is a strictly anaerobic, Gram-negative, polysaccharides-degrading ruminal bacterium. EDTA/sphaeroplasting and osmotic shock procedure were used in present work to localise endoxylanolytic, b -xylosidase and a -L-arabinofuranosidase activities of P. bryantii B14. Late exponential phase cells released most of the endoxylanolytic and CMC-ase activity by osmotic shock, showing the periplasmic location of both enzymatic activities, while b -xylosidase and a -L-arabinofuranosidase activities were recovered largely in membrane cell fraction. About 23 % of the total culture endoxylanase activity and about 30 % of the CMC-ase activity were found to be extracellular. No b -xylosidase and a -L-arabinofuranosidase activities were detected in culture supernatant of P. bryantii B14.
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| format | Article |
| id | doaj-art-6228f4b0ded947eaa1efe74c331fb0a5 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 1997-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-6228f4b0ded947eaa1efe74c331fb0a52025-08-20T02:57:02ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411997-12-0170110.14720/aas.1997.70.1.16053On the localization of xylanolytic enzymes in Prevotella Bryantii B14Romana MARINŠEK-LOGAR0Franc Viktor NEKREP1Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia Prevotella bryantii B14 is a strictly anaerobic, Gram-negative, polysaccharides-degrading ruminal bacterium. EDTA/sphaeroplasting and osmotic shock procedure were used in present work to localise endoxylanolytic, b -xylosidase and a -L-arabinofuranosidase activities of P. bryantii B14. Late exponential phase cells released most of the endoxylanolytic and CMC-ase activity by osmotic shock, showing the periplasmic location of both enzymatic activities, while b -xylosidase and a -L-arabinofuranosidase activities were recovered largely in membrane cell fraction. About 23 % of the total culture endoxylanase activity and about 30 % of the CMC-ase activity were found to be extracellular. No b -xylosidase and a -L-arabinofuranosidase activities were detected in culture supernatant of P. bryantii B14. https://journals.uni-lj.si/aas/article/view/16053rumenanaerobic bacteriaPrevotella bryantiixylanasesenzyme localization |
| spellingShingle | Romana MARINŠEK-LOGAR Franc Viktor NEKREP On the localization of xylanolytic enzymes in Prevotella Bryantii B14 Acta Agriculturae Slovenica rumen anaerobic bacteria Prevotella bryantii xylanases enzyme localization |
| title | On the localization of xylanolytic enzymes in Prevotella Bryantii B14 |
| title_full | On the localization of xylanolytic enzymes in Prevotella Bryantii B14 |
| title_fullStr | On the localization of xylanolytic enzymes in Prevotella Bryantii B14 |
| title_full_unstemmed | On the localization of xylanolytic enzymes in Prevotella Bryantii B14 |
| title_short | On the localization of xylanolytic enzymes in Prevotella Bryantii B14 |
| title_sort | on the localization of xylanolytic enzymes in prevotella bryantii b14 |
| topic | rumen anaerobic bacteria Prevotella bryantii xylanases enzyme localization |
| url | https://journals.uni-lj.si/aas/article/view/16053 |
| work_keys_str_mv | AT romanamarinseklogar onthelocalizationofxylanolyticenzymesinprevotellabryantiib14 AT francviktornekrep onthelocalizationofxylanolyticenzymesinprevotellabryantiib14 |