Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats
This study investigated the probable protective effects of resveratrol (RES), an antioxidant, against methotrexate- (MTX-) induced testis damage. Twenty-four male Sprague Dawley rats were randomly divided into four groups: control, RES, MTX, and MTX + RES groups. Rats were sacrificed at the end of t...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/489659 |
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| author | Esin Yuluğ Sibel Türedi Ahmet Alver Süleyman Türedi Cemil Kahraman |
| author_facet | Esin Yuluğ Sibel Türedi Ahmet Alver Süleyman Türedi Cemil Kahraman |
| author_sort | Esin Yuluğ |
| collection | DOAJ |
| description | This study investigated the probable protective effects of resveratrol (RES), an antioxidant, against methotrexate- (MTX-) induced testis damage. Twenty-four male Sprague Dawley rats were randomly divided into four groups: control, RES, MTX, and MTX + RES groups. Rats were sacrificed at the end of the experiment. Plasma and tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activity in tissue, testicular histopathological damage scores, and testicular and epididymal epithelial apoptotic index (AI) were evaluated. The MTX group had significantly higher plasma and tissue MDA levels and significantly lower SOD and CAT activity than those of the control group. In the MTX + RES group, plasma and tissue MDA levels decreased significantly and SOD activity rose significantly compared to the MTX group. The MTX group had significantly lower Johnsen’s testicular biopsy score (JTBS) values than those of the control group. JTBS was significantly higher in the MTX + RES group than in the MTX group. AI increased in the testis and epididymis in the MTX group and significantly decreased in the MTX + RES group. Our results indicate that RES has protective effects against MTX-induced testis damage at the biochemical, histopathological, and apoptotic levels. |
| format | Article |
| id | doaj-art-621fd6d62f8d4526815fae2409fffad9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-621fd6d62f8d4526815fae2409fffad92025-02-03T01:26:17ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/489659489659Effects of Resveratrol on Methotrexate-Induced Testicular Damage in RatsEsin Yuluğ0Sibel Türedi1Ahmet Alver2Süleyman Türedi3Cemil Kahraman4Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Emergency Medicine, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, TurkeyThis study investigated the probable protective effects of resveratrol (RES), an antioxidant, against methotrexate- (MTX-) induced testis damage. Twenty-four male Sprague Dawley rats were randomly divided into four groups: control, RES, MTX, and MTX + RES groups. Rats were sacrificed at the end of the experiment. Plasma and tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activity in tissue, testicular histopathological damage scores, and testicular and epididymal epithelial apoptotic index (AI) were evaluated. The MTX group had significantly higher plasma and tissue MDA levels and significantly lower SOD and CAT activity than those of the control group. In the MTX + RES group, plasma and tissue MDA levels decreased significantly and SOD activity rose significantly compared to the MTX group. The MTX group had significantly lower Johnsen’s testicular biopsy score (JTBS) values than those of the control group. JTBS was significantly higher in the MTX + RES group than in the MTX group. AI increased in the testis and epididymis in the MTX group and significantly decreased in the MTX + RES group. Our results indicate that RES has protective effects against MTX-induced testis damage at the biochemical, histopathological, and apoptotic levels.http://dx.doi.org/10.1155/2013/489659 |
| spellingShingle | Esin Yuluğ Sibel Türedi Ahmet Alver Süleyman Türedi Cemil Kahraman Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats The Scientific World Journal |
| title | Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats |
| title_full | Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats |
| title_fullStr | Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats |
| title_full_unstemmed | Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats |
| title_short | Effects of Resveratrol on Methotrexate-Induced Testicular Damage in Rats |
| title_sort | effects of resveratrol on methotrexate induced testicular damage in rats |
| url | http://dx.doi.org/10.1155/2013/489659 |
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