Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis.
Polycomb group proteins mediate transcriptional silencing in diverse developmental processes. Sex chromosomes undergo chromosome-wide transcription silencing during male meiosis. Here we report that mouse SCML2 (Sex comb on midleg-like 2), an X chromosome-encoded polycomb protein, is specifically ex...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004954&type=printable |
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| author | Mengcheng Luo Jian Zhou N Adrian Leu Carla M Abreu Jianle Wang Montserrat C Anguera Dirk G de Rooij Maria Jasin P Jeremy Wang |
| author_facet | Mengcheng Luo Jian Zhou N Adrian Leu Carla M Abreu Jianle Wang Montserrat C Anguera Dirk G de Rooij Maria Jasin P Jeremy Wang |
| author_sort | Mengcheng Luo |
| collection | DOAJ |
| description | Polycomb group proteins mediate transcriptional silencing in diverse developmental processes. Sex chromosomes undergo chromosome-wide transcription silencing during male meiosis. Here we report that mouse SCML2 (Sex comb on midleg-like 2), an X chromosome-encoded polycomb protein, is specifically expressed in germ cells, including spermatogonia, spermatocytes, and round spermatids. SCML2 associates with phosphorylated H2AX and localizes to the XY body in spermatocytes. Loss of SCML2 in mice causes defective spermatogenesis, resulting in sharply reduced sperm production. SCML2 interacts with and recruits a deubiquitinase, USP7, to the XY body in spermatocytes. In the absence of SCML2, USP7 fails to accumulate on the XY body, whereas H2A monoubiquitination is dramatically augmented in the XY chromatin. Our results demonstrate that the SCML2/USP7 complex constitutes a novel molecular pathway in modulating the epigenetic state of sex chromosomes during male meiosis. |
| format | Article |
| id | doaj-art-621c5b5e66ee41edb4a88fdf68d700cc |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-621c5b5e66ee41edb4a88fdf68d700cc2025-08-20T02:15:13ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-01-01111e100495410.1371/journal.pgen.1004954Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis.Mengcheng LuoJian ZhouN Adrian LeuCarla M AbreuJianle WangMontserrat C AngueraDirk G de RooijMaria JasinP Jeremy WangPolycomb group proteins mediate transcriptional silencing in diverse developmental processes. Sex chromosomes undergo chromosome-wide transcription silencing during male meiosis. Here we report that mouse SCML2 (Sex comb on midleg-like 2), an X chromosome-encoded polycomb protein, is specifically expressed in germ cells, including spermatogonia, spermatocytes, and round spermatids. SCML2 associates with phosphorylated H2AX and localizes to the XY body in spermatocytes. Loss of SCML2 in mice causes defective spermatogenesis, resulting in sharply reduced sperm production. SCML2 interacts with and recruits a deubiquitinase, USP7, to the XY body in spermatocytes. In the absence of SCML2, USP7 fails to accumulate on the XY body, whereas H2A monoubiquitination is dramatically augmented in the XY chromatin. Our results demonstrate that the SCML2/USP7 complex constitutes a novel molecular pathway in modulating the epigenetic state of sex chromosomes during male meiosis.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004954&type=printable |
| spellingShingle | Mengcheng Luo Jian Zhou N Adrian Leu Carla M Abreu Jianle Wang Montserrat C Anguera Dirk G de Rooij Maria Jasin P Jeremy Wang Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. PLoS Genetics |
| title | Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. |
| title_full | Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. |
| title_fullStr | Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. |
| title_full_unstemmed | Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. |
| title_short | Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. |
| title_sort | polycomb protein scml2 associates with usp7 and counteracts histone h2a ubiquitination in the xy chromatin during male meiosis |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1004954&type=printable |
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