Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury
Neuronal apoptosis is the main pathological feature of spinal cord injury (SCI), while autophagy contributes to ameliorating neuronal damage via inhibition of apoptosis. Here, we investigated the role of tectonic family member 2 (TCTN2) long non‐coding RNA on apoptosis and autophagy in SCI. TCTN2 wa...
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| Format: | Article |
| Language: | English |
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Wiley
2019-07-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12651 |
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| author | Xiao‐dong Ren Chun‐xiao Wan Ya‐li Niu |
| author_facet | Xiao‐dong Ren Chun‐xiao Wan Ya‐li Niu |
| author_sort | Xiao‐dong Ren |
| collection | DOAJ |
| description | Neuronal apoptosis is the main pathological feature of spinal cord injury (SCI), while autophagy contributes to ameliorating neuronal damage via inhibition of apoptosis. Here, we investigated the role of tectonic family member 2 (TCTN2) long non‐coding RNA on apoptosis and autophagy in SCI. TCTN2 was down‐regulated in the spinal cord tissues of a rat model of SCI and in oxygen–glucose deprivation‐induced hypoxic SY‐SH‐5Y cells, while microRNA‐216b (miR‐216b) was up‐regulated. Overexpression of TCTN2 reduced neuron apoptosis by inducing autophagy, and TCTN2 was observed to negatively regulate miR‐216b. Furthermore, TCTN2 promoted autophagy to repress apoptosis through the miR‐216b–Beclin‐1 pathway, and overexpression of TCTN2 improved neurological function in the SCI rat model. In summary, our data suggest that TCTN2 enhances autophagy by targeting the miR‐216b–Beclin‐1 pathway, thereby ameliorating neuronal apoptosis and relieving spinal cord injury. |
| format | Article |
| id | doaj-art-621b71e8971c48b4a63f03cb53b5c0b3 |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2019-07-01 |
| publisher | Wiley |
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| series | FEBS Open Bio |
| spelling | doaj-art-621b71e8971c48b4a63f03cb53b5c0b32025-08-20T02:37:29ZengWileyFEBS Open Bio2211-54632019-07-01971223123110.1002/2211-5463.12651Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injuryXiao‐dong Ren0Chun‐xiao Wan1Ya‐li Niu2Department of Rehabilitation The General Hospital Tianjin Medical University ChinaDepartment of Rehabilitation The General Hospital Tianjin Medical University ChinaDepartment of Rehabilitation The General Hospital Tianjin Medical University ChinaNeuronal apoptosis is the main pathological feature of spinal cord injury (SCI), while autophagy contributes to ameliorating neuronal damage via inhibition of apoptosis. Here, we investigated the role of tectonic family member 2 (TCTN2) long non‐coding RNA on apoptosis and autophagy in SCI. TCTN2 was down‐regulated in the spinal cord tissues of a rat model of SCI and in oxygen–glucose deprivation‐induced hypoxic SY‐SH‐5Y cells, while microRNA‐216b (miR‐216b) was up‐regulated. Overexpression of TCTN2 reduced neuron apoptosis by inducing autophagy, and TCTN2 was observed to negatively regulate miR‐216b. Furthermore, TCTN2 promoted autophagy to repress apoptosis through the miR‐216b–Beclin‐1 pathway, and overexpression of TCTN2 improved neurological function in the SCI rat model. In summary, our data suggest that TCTN2 enhances autophagy by targeting the miR‐216b–Beclin‐1 pathway, thereby ameliorating neuronal apoptosis and relieving spinal cord injury.https://doi.org/10.1002/2211-5463.12651autophagyBeclin‐1miR‐216bneuronal apoptosisspinal cord injuryTCTN2 |
| spellingShingle | Xiao‐dong Ren Chun‐xiao Wan Ya‐li Niu Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury FEBS Open Bio autophagy Beclin‐1 miR‐216b neuronal apoptosis spinal cord injury TCTN2 |
| title | Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| title_full | Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| title_fullStr | Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| title_full_unstemmed | Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| title_short | Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| title_sort | overexpression of lncrna tctn2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury |
| topic | autophagy Beclin‐1 miR‐216b neuronal apoptosis spinal cord injury TCTN2 |
| url | https://doi.org/10.1002/2211-5463.12651 |
| work_keys_str_mv | AT xiaodongren overexpressionoflncrnatctn2protectsneuronsfromapoptosisbyenhancingcellautophagyinspinalcordinjury AT chunxiaowan overexpressionoflncrnatctn2protectsneuronsfromapoptosisbyenhancingcellautophagyinspinalcordinjury AT yaliniu overexpressionoflncrnatctn2protectsneuronsfromapoptosisbyenhancingcellautophagyinspinalcordinjury |