FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia

Abstract Navigating the duality of opioids’ potent analgesia and side effects, including tolerance and hyperalgesia, is a significant challenge in chronic pain management, often prompting hazardous dose escalation to maintain analgesic effects. The peripheral mu-opioid receptor (MOR) is known to med...

Full description

Saved in:
Bibliographic Details
Main Authors: Antoine Jouvenel, Adrien Tassou, Maxime Thouaye, Jérôme Ruel, Myriam Antri, Jean-Philippe Leyris, Aurore Giraudin, Sylvie Mallié, Chamroeum Sar, Lucie Diouloufet, Corinne Sonrier, François Daubeuf, Juliette Bertin, Stacy Alves, Stéphanie Ventéo, Nelly Frossard, Patrick Carroll, Ilana Mechaly, Didier Rognan, Pierre Sokoloff, Radhouane Dallel, Patrick Delmas, Jean Valmier, Cyril Rivat
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54054-y
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846171813453758464
author Antoine Jouvenel
Adrien Tassou
Maxime Thouaye
Jérôme Ruel
Myriam Antri
Jean-Philippe Leyris
Aurore Giraudin
Sylvie Mallié
Chamroeum Sar
Lucie Diouloufet
Corinne Sonrier
François Daubeuf
Juliette Bertin
Stacy Alves
Stéphanie Ventéo
Nelly Frossard
Patrick Carroll
Ilana Mechaly
Didier Rognan
Pierre Sokoloff
Radhouane Dallel
Patrick Delmas
Jean Valmier
Cyril Rivat
author_facet Antoine Jouvenel
Adrien Tassou
Maxime Thouaye
Jérôme Ruel
Myriam Antri
Jean-Philippe Leyris
Aurore Giraudin
Sylvie Mallié
Chamroeum Sar
Lucie Diouloufet
Corinne Sonrier
François Daubeuf
Juliette Bertin
Stacy Alves
Stéphanie Ventéo
Nelly Frossard
Patrick Carroll
Ilana Mechaly
Didier Rognan
Pierre Sokoloff
Radhouane Dallel
Patrick Delmas
Jean Valmier
Cyril Rivat
author_sort Antoine Jouvenel
collection DOAJ
description Abstract Navigating the duality of opioids’ potent analgesia and side effects, including tolerance and hyperalgesia, is a significant challenge in chronic pain management, often prompting hazardous dose escalation to maintain analgesic effects. The peripheral mu-opioid receptor (MOR) is known to mediate these contradictory effects. Here, we show that the fms-like tyrosine kinase receptor 3 (FLT3) in peripheral somatosensory neurons drives morphine tolerance and hyperalgesia in a male rodent model. We found that chronic morphine treatment increases FLT3 and MOR co-expression, and that inhibiting FLT3 represses MOR-induced hyperactivation of the cyclic adenosine monophosphate (cAMP) signaling pathway, mitigating maladaptive excitatory processes engaged after chronic morphine treatment. Furthermore, in postsurgical or inflammatory models of chronic pain, co-administering morphine with a FLT3-specific inhibitor not only prevents or suppresses tolerance and hyperalgesia but also potentiates the analgesic efficacy of morphine, without aggravating other morphine-induced adverse effects. Our findings suggest that pairing morphine with FLT3 inhibitors could become a promising avenue for chronic pain management to safely harness the power of opioids, without the risk of dose escalation. By enhancing morphine analgesic potency through FLT3 inhibition, this approach could minimize opioid dosage, thereby curtailing the risk of addiction and other opioid-related side effects.
format Article
id doaj-art-62199c8ab61a47228dbb08f3a3507de8
institution Kabale University
issn 2041-1723
language English
publishDate 2024-11-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-62199c8ab61a47228dbb08f3a3507de82024-11-10T12:33:34ZengNature PortfolioNature Communications2041-17232024-11-0115111510.1038/s41467-024-54054-yFLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesiaAntoine Jouvenel0Adrien Tassou1Maxime Thouaye2Jérôme Ruel3Myriam Antri4Jean-Philippe Leyris5Aurore Giraudin6Sylvie Mallié7Chamroeum Sar8Lucie Diouloufet9Corinne Sonrier10François Daubeuf11Juliette Bertin12Stacy Alves13Stéphanie Ventéo14Nelly Frossard15Patrick Carroll16Ilana Mechaly17Didier Rognan18Pierre Sokoloff19Radhouane Dallel20Patrick Delmas21Jean Valmier22Cyril Rivat23Université de MontpellierUniversité de MontpellierUniversité de MontpellierCentre de Recherche en CardioVasculaire et Nutrition, Aix-Marseille Université - INSERM 1263 -INRAE 1260NeuroDol INSERM 1107Biodol TherapeuticsNeuroDol INSERM 1107Inserm U−1298, Institut des Neurosciences de MontpellierUniversité de MontpellierInserm U−1298, Institut des Neurosciences de MontpellierInserm U−1298, Institut des Neurosciences de MontpellierLaboratoire d’Innovation Thérapeutique, UMR7200 CNRS/Université de StrasbourgInserm U−1298, Institut des Neurosciences de MontpellierUniversité de MontpellierUniversité de MontpellierLaboratoire d’Innovation Thérapeutique, UMR7200 CNRS/Université de StrasbourgUniversité de MontpellierUniversité de MontpellierLaboratoire d’Innovation Thérapeutique, UMR7200 CNRS/Université de StrasbourgBiodol TherapeuticsNeuroDol INSERM 1107Centre de Recherche en CardioVasculaire et Nutrition, Aix-Marseille Université - INSERM 1263 -INRAE 1260Université de MontpellierUniversité de MontpellierAbstract Navigating the duality of opioids’ potent analgesia and side effects, including tolerance and hyperalgesia, is a significant challenge in chronic pain management, often prompting hazardous dose escalation to maintain analgesic effects. The peripheral mu-opioid receptor (MOR) is known to mediate these contradictory effects. Here, we show that the fms-like tyrosine kinase receptor 3 (FLT3) in peripheral somatosensory neurons drives morphine tolerance and hyperalgesia in a male rodent model. We found that chronic morphine treatment increases FLT3 and MOR co-expression, and that inhibiting FLT3 represses MOR-induced hyperactivation of the cyclic adenosine monophosphate (cAMP) signaling pathway, mitigating maladaptive excitatory processes engaged after chronic morphine treatment. Furthermore, in postsurgical or inflammatory models of chronic pain, co-administering morphine with a FLT3-specific inhibitor not only prevents or suppresses tolerance and hyperalgesia but also potentiates the analgesic efficacy of morphine, without aggravating other morphine-induced adverse effects. Our findings suggest that pairing morphine with FLT3 inhibitors could become a promising avenue for chronic pain management to safely harness the power of opioids, without the risk of dose escalation. By enhancing morphine analgesic potency through FLT3 inhibition, this approach could minimize opioid dosage, thereby curtailing the risk of addiction and other opioid-related side effects.https://doi.org/10.1038/s41467-024-54054-y
spellingShingle Antoine Jouvenel
Adrien Tassou
Maxime Thouaye
Jérôme Ruel
Myriam Antri
Jean-Philippe Leyris
Aurore Giraudin
Sylvie Mallié
Chamroeum Sar
Lucie Diouloufet
Corinne Sonrier
François Daubeuf
Juliette Bertin
Stacy Alves
Stéphanie Ventéo
Nelly Frossard
Patrick Carroll
Ilana Mechaly
Didier Rognan
Pierre Sokoloff
Radhouane Dallel
Patrick Delmas
Jean Valmier
Cyril Rivat
FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
Nature Communications
title FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
title_full FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
title_fullStr FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
title_full_unstemmed FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
title_short FLT3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
title_sort flt3 signaling inhibition abrogates opioid tolerance and hyperalgesia while preserving analgesia
url https://doi.org/10.1038/s41467-024-54054-y
work_keys_str_mv AT antoinejouvenel flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT adrientassou flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT maximethouaye flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT jeromeruel flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT myriamantri flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT jeanphilippeleyris flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT auroregiraudin flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT sylviemallie flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT chamroeumsar flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT luciediouloufet flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT corinnesonrier flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT francoisdaubeuf flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT juliettebertin flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT stacyalves flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT stephanieventeo flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT nellyfrossard flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT patrickcarroll flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT ilanamechaly flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT didierrognan flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT pierresokoloff flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT radhouanedallel flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT patrickdelmas flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT jeanvalmier flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia
AT cyrilrivat flt3signalinginhibitionabrogatesopioidtoleranceandhyperalgesiawhilepreservinganalgesia