Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears
OBJECTIVES: The Wnt/β-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues. The aim of the study is to evaluate the activity of Wnt/β-catenin signaling in cervical smears by using immunocytochemistry. STUDY DESIGN: Cervical smears taken from 200 patients were...
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| Language: | English |
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Medical Network
2013-08-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/193 |
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| _version_ | 1823857349743345664 |
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| author | Hanife Güler Dönmez Şayeste Demirezen Sinan Beksaç |
| author_facet | Hanife Güler Dönmez Şayeste Demirezen Sinan Beksaç |
| author_sort | Hanife Güler Dönmez |
| collection | DOAJ |
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OBJECTIVES: The Wnt/β-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues. The aim of the study is to evaluate the activity of Wnt/β-catenin signaling in cervical smears by using immunocytochemistry.
STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostaining of β-catenin. The membranous staining with moderate (++) or strong (+++) cytoplasmic and/or nuclear staining was scored positive for signaling activity.
RESULTS: Positive Wnt/β-catenin signaling was found in 21 of 200 cases (10.5%). The nucleus was not found to be positive in any of the cells. However, some nuclear membranes were positive. In addition, when signaling was active, the membranous expression of β-catenin was observed to increase by a statistically significant amount epithelial cells (p<0.05).
CONCLUSION: Our data indicates that immunocytochemical techniques can be used to detect the activity of the Wnt/β-catenin signaling pathway in routine cervical smears.
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| format | Article |
| id | doaj-art-6211c4e7a5fd4445a8d9261386aabf2f |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2013-08-01 |
| publisher | Medical Network |
| record_format | Article |
| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-6211c4e7a5fd4445a8d9261386aabf2f2025-02-11T21:20:46ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182013-08-01192132Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical SmearsHanife Güler Dönmez0Şayeste Demirezen1Sinan Beksaç2Hacettepe University Faculty of Natural & Applied Science Department of Biology, AnkaraHacettepe University Faculty of Natural & Applied Science Department of Biology, AnkaraHacettepe University, Faculty of Medicine Department of Obstetrics and Gynecology, Ankara OBJECTIVES: The Wnt/β-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues. The aim of the study is to evaluate the activity of Wnt/β-catenin signaling in cervical smears by using immunocytochemistry. STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostaining of β-catenin. The membranous staining with moderate (++) or strong (+++) cytoplasmic and/or nuclear staining was scored positive for signaling activity. RESULTS: Positive Wnt/β-catenin signaling was found in 21 of 200 cases (10.5%). The nucleus was not found to be positive in any of the cells. However, some nuclear membranes were positive. In addition, when signaling was active, the membranous expression of β-catenin was observed to increase by a statistically significant amount epithelial cells (p<0.05). CONCLUSION: Our data indicates that immunocytochemical techniques can be used to detect the activity of the Wnt/β-catenin signaling pathway in routine cervical smears. https://gorm.com.tr/index.php/GORM/article/view/193Wnt signalingBeta-cateninCervical smearImmunocytochemistry |
| spellingShingle | Hanife Güler Dönmez Şayeste Demirezen Sinan Beksaç Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears Gynecology Obstetrics & Reproductive Medicine Wnt signaling Beta-catenin Cervical smear Immunocytochemistry |
| title | Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears |
| title_full | Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears |
| title_fullStr | Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears |
| title_full_unstemmed | Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears |
| title_short | Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears |
| title_sort | detection of the wnt beta catenin signaling activity by using immunocytochemical technique in cervical smears |
| topic | Wnt signaling Beta-catenin Cervical smear Immunocytochemistry |
| url | https://gorm.com.tr/index.php/GORM/article/view/193 |
| work_keys_str_mv | AT hanifegulerdonmez detectionofthewntbetacateninsignalingactivitybyusingimmunocytochemicaltechniqueincervicalsmears AT sayestedemirezen detectionofthewntbetacateninsignalingactivitybyusingimmunocytochemicaltechniqueincervicalsmears AT sinanbeksac detectionofthewntbetacateninsignalingactivitybyusingimmunocytochemicaltechniqueincervicalsmears |