Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects
Cellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardio...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Cardiovascular Therapeutics |
| Online Access: | http://dx.doi.org/10.1155/2021/5577218 |
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| author | Azin Aghamajidi Hesam Babaei Khameneh Narges Amirjamshidi Seyed Farzad Jalali Haleh Akhavan-Niaki Soraya Khafri Seyedeh Narges Mousavi Monireh Golpour Maryam Mehri AmrollahMostafazadeh |
| author_facet | Azin Aghamajidi Hesam Babaei Khameneh Narges Amirjamshidi Seyed Farzad Jalali Haleh Akhavan-Niaki Soraya Khafri Seyedeh Narges Mousavi Monireh Golpour Maryam Mehri AmrollahMostafazadeh |
| author_sort | Azin Aghamajidi |
| collection | DOAJ |
| description | Cellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardiovascular function. Hence, the aim of this study was to show a potential therapeutic application of proteins derived from heat-stressed fibroblast in CAD patients. Fibroblasts were isolated from the foreskin and cultured under heat stress conditions. Surprisingly, 1.06% of the cells exhibited a necrotic death pattern. Furthermore, heat-stressed fibroblasts produced higher level of total proteins than control cells. In SDS-PAGE analysis, a 70 kDa protein band was observed in stressed cell culture supernatants which appeared as two acidic spots with close pI in the two-dimensional electrophoresis. To evaluate the immunogenic properties of fibroblast-derived heat shock proteins (HSPs), the serum immunoglobulin-G (IgG) was measured by ELISA in 50 CAD patients and 50 normal subjects who had been diagnosed through angiography. Interestingly, the level of anti-HSP antibody was significantly higher in non-CAD individuals in comparison with the patient’s group (p<0.05). The odds ratio for CAD was 5.06 (95%CI=2.15‐11.91) in cut-off value of 30 AU/mL of anti-HSP antibody. Moreover, ROC analysis showed that anti-HSP antibodies had a specificity of 74% and a sensitivity of 64%, which is almost equal to 66% sensitivity of exercise stress test (EST) as a CAD diagnostic method. These data revealed that fibroblast-derived HSPs are suitable for the diagnosis and management of CAD through antibody production. |
| format | Article |
| id | doaj-art-6204703c8d2e43318f3ea80fec47000c |
| institution | OA Journals |
| issn | 1755-5914 1755-5922 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiovascular Therapeutics |
| spelling | doaj-art-6204703c8d2e43318f3ea80fec47000c2025-08-20T02:24:01ZengWileyCardiovascular Therapeutics1755-59141755-59222021-01-01202110.1155/2021/55772185577218Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal SubjectsAzin Aghamajidi0Hesam Babaei Khameneh1Narges Amirjamshidi2Seyed Farzad Jalali3Haleh Akhavan-Niaki4Soraya Khafri5Seyedeh Narges Mousavi6Monireh Golpour7Maryam Mehri8AmrollahMostafazadeh9Students Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, IranStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, IranStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, IranDepartment of Cardiology, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, IranCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranDepartment of Social Medicine, Babol University of Medical Sciences, Babol, IranCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranMolecular and Cell Biology Research Center, Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Science, Sari, IranStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, IranCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranCellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardiovascular function. Hence, the aim of this study was to show a potential therapeutic application of proteins derived from heat-stressed fibroblast in CAD patients. Fibroblasts were isolated from the foreskin and cultured under heat stress conditions. Surprisingly, 1.06% of the cells exhibited a necrotic death pattern. Furthermore, heat-stressed fibroblasts produced higher level of total proteins than control cells. In SDS-PAGE analysis, a 70 kDa protein band was observed in stressed cell culture supernatants which appeared as two acidic spots with close pI in the two-dimensional electrophoresis. To evaluate the immunogenic properties of fibroblast-derived heat shock proteins (HSPs), the serum immunoglobulin-G (IgG) was measured by ELISA in 50 CAD patients and 50 normal subjects who had been diagnosed through angiography. Interestingly, the level of anti-HSP antibody was significantly higher in non-CAD individuals in comparison with the patient’s group (p<0.05). The odds ratio for CAD was 5.06 (95%CI=2.15‐11.91) in cut-off value of 30 AU/mL of anti-HSP antibody. Moreover, ROC analysis showed that anti-HSP antibodies had a specificity of 74% and a sensitivity of 64%, which is almost equal to 66% sensitivity of exercise stress test (EST) as a CAD diagnostic method. These data revealed that fibroblast-derived HSPs are suitable for the diagnosis and management of CAD through antibody production.http://dx.doi.org/10.1155/2021/5577218 |
| spellingShingle | Azin Aghamajidi Hesam Babaei Khameneh Narges Amirjamshidi Seyed Farzad Jalali Haleh Akhavan-Niaki Soraya Khafri Seyedeh Narges Mousavi Monireh Golpour Maryam Mehri AmrollahMostafazadeh Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects Cardiovascular Therapeutics |
| title | Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects |
| title_full | Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects |
| title_fullStr | Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects |
| title_full_unstemmed | Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects |
| title_short | Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects |
| title_sort | patients with coronary artery disease have lower levels of antibody to heat stressed fibroblast derived proteins versus normal subjects |
| url | http://dx.doi.org/10.1155/2021/5577218 |
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