Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice
To evaluate the efficacy of human placenta hydrolysate (HPH) in a mice model of CFA-induced inflammatory pain. TNF-α, IL-1β, and IL-6 are key pro-inflammatory cytokine factors for relieving inflammatory pain. Therefore, this study investigates whether HPH suppresses CFA-induced pain and attenuates t...
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2024-09-01
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| author | Keun-Tae Park Heejoon Jo So-Hyun Jeon Kyeongsoo Jeong Minju Im Jae-Won Kim Jong-Pil Jung Hoe Chang Jung Jae hun Lee Woojin Kim |
| author_facet | Keun-Tae Park Heejoon Jo So-Hyun Jeon Kyeongsoo Jeong Minju Im Jae-Won Kim Jong-Pil Jung Hoe Chang Jung Jae hun Lee Woojin Kim |
| author_sort | Keun-Tae Park |
| collection | DOAJ |
| description | To evaluate the efficacy of human placenta hydrolysate (HPH) in a mice model of CFA-induced inflammatory pain. TNF-α, IL-1β, and IL-6 are key pro-inflammatory cytokine factors for relieving inflammatory pain. Therefore, this study investigates whether HPH suppresses CFA-induced pain and attenuates the inflammatory process by regulating cytokines. In addition, the relationship between neuropathic pain and HPH was established by staining GFAP and Iba-1 in mice spinal cord tissues. This study was conducted for a total of day 28, and inflammatory pain was induced in mice by injecting CFA into the right paw at day 0 and day 14, respectively. 100 μL of 20% glucose and polydeoxyribonucleotide (PDRN) and 100, 200, and 300 μL of HPH were administered intraperitoneally twice a week. In the CFA-induced group, cold and mechanical allodynia and pro-inflammatory cytokine factors in the spinal cord and plantar tissue were significantly increased. The five groups of drugs evenly reduced pain and gene expression of inflammatory factors, and particularly excellent effects were confirmed in the HPH 200 and 300 groups. Meanwhile, the expression of GFAP and Iba-1 in the spinal cord was increased by CFA administration but decreased by HPH administration, which was confirmed to suppress damage to peripheral ganglia. The present study suggests that HPH attenuates CFA-induced inflammatory pain through inhibition of pro-inflammatory cytokine factors and protection of peripheral nerves. |
| format | Article |
| id | doaj-art-61eaa65cbe0f474c824d04195b054935 |
| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-09-01 |
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| series | Pharmaceuticals |
| spelling | doaj-art-61eaa65cbe0f474c824d04195b0549352025-08-20T01:55:46ZengMDPI AGPharmaceuticals1424-82472024-09-01179117910.3390/ph17091179Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in MiceKeun-Tae Park0Heejoon Jo1So-Hyun Jeon2Kyeongsoo Jeong3Minju Im4Jae-Won Kim5Jong-Pil Jung6Hoe Chang Jung7Jae hun Lee8Woojin Kim9Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, Republic of KoreaResearch and Development Center, Green Cross Wellbeing Corporation, Yongin 16950, Republic of KoreaResearch and Development Center, Green Cross Wellbeing Corporation, Yongin 16950, Republic of KoreaResearch and Development Center, Green Cross Wellbeing Corporation, Yongin 16950, Republic of KoreaResearch and Development Center, Green Cross Wellbeing Corporation, Yongin 16950, Republic of KoreaNuke Medical Society of Pain Research, Daejeon 35002, Republic of KoreaNuke Medical Society of Pain Research, Daejeon 35002, Republic of KoreaNuke Medical Society of Pain Research, Daejeon 35002, Republic of KoreaDepartment of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, Republic of KoreaTo evaluate the efficacy of human placenta hydrolysate (HPH) in a mice model of CFA-induced inflammatory pain. TNF-α, IL-1β, and IL-6 are key pro-inflammatory cytokine factors for relieving inflammatory pain. Therefore, this study investigates whether HPH suppresses CFA-induced pain and attenuates the inflammatory process by regulating cytokines. In addition, the relationship between neuropathic pain and HPH was established by staining GFAP and Iba-1 in mice spinal cord tissues. This study was conducted for a total of day 28, and inflammatory pain was induced in mice by injecting CFA into the right paw at day 0 and day 14, respectively. 100 μL of 20% glucose and polydeoxyribonucleotide (PDRN) and 100, 200, and 300 μL of HPH were administered intraperitoneally twice a week. In the CFA-induced group, cold and mechanical allodynia and pro-inflammatory cytokine factors in the spinal cord and plantar tissue were significantly increased. The five groups of drugs evenly reduced pain and gene expression of inflammatory factors, and particularly excellent effects were confirmed in the HPH 200 and 300 groups. Meanwhile, the expression of GFAP and Iba-1 in the spinal cord was increased by CFA administration but decreased by HPH administration, which was confirmed to suppress damage to peripheral ganglia. The present study suggests that HPH attenuates CFA-induced inflammatory pain through inhibition of pro-inflammatory cytokine factors and protection of peripheral nerves.https://www.mdpi.com/1424-8247/17/9/1179human placenta hydrolysateinflammatory painpro-inflammatory cytokineglial cell |
| spellingShingle | Keun-Tae Park Heejoon Jo So-Hyun Jeon Kyeongsoo Jeong Minju Im Jae-Won Kim Jong-Pil Jung Hoe Chang Jung Jae hun Lee Woojin Kim Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice Pharmaceuticals human placenta hydrolysate inflammatory pain pro-inflammatory cytokine glial cell |
| title | Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice |
| title_full | Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice |
| title_fullStr | Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice |
| title_full_unstemmed | Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice |
| title_short | Analgesic Effect of Human Placenta Hydrolysate on CFA-Induced Inflammatory Pain in Mice |
| title_sort | analgesic effect of human placenta hydrolysate on cfa induced inflammatory pain in mice |
| topic | human placenta hydrolysate inflammatory pain pro-inflammatory cytokine glial cell |
| url | https://www.mdpi.com/1424-8247/17/9/1179 |
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