Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant

Two experiments were conducted to assess the effect of kinetin and NAA on the shoot growth and rooting of tissue-cultured Aglaonema tricolor using leaf bud explant. Likewise, it was also aimed to assess the suitability of Aglaonema leaf bud as explant and to determine the amount of NAA and kinetin n...

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Main Author: Susan Labasano
Format: Article
Language:English
Published: Davao Oriental State University 2018-12-01
Series:Davao Research Journal
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Online Access:https://davaoresearchjournal.ph/index.php/main/article/view/12
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author Susan Labasano
author_facet Susan Labasano
author_sort Susan Labasano
collection DOAJ
description Two experiments were conducted to assess the effect of kinetin and NAA on the shoot growth and rooting of tissue-cultured Aglaonema tricolor using leaf bud explant. Likewise, it was also aimed to assess the suitability of Aglaonema leaf bud as explant and to determine the amount of NAA and kinetin needed in shoot formation and proliferation of the said foliage ornamental crop. NAA applied singly or in combination with kinetin did not affect the percentage survival of the explants, percent shoot formation, number of shoots, length of shoots, days to shoot formation and percent callus formation. Rates used were 0, 1.0 and 1.5 ppm kinetin and 0.25, and 5 ppm NAA A comparative evaluation of NAA and Kinetin was also conducted. In the follow-up study, percent callus formation, shoot length, number, and length of roots were significantly higher which showed a better effect with the application of 5 ppm NAA than 1.5 ppm Kinetin + 2.5 ppm NAA. In the first study, the optimum amount and the effective ratio of NAA and kinetin to enhance growth may not have been the ones tested. It is also possible that the variety used was not responsive to the growth regulator. The result of this study would add information on the use of growth regulators in the micropropagation of foliage crops. Studies like this will promote the advancement of foliage crop propagation for commercialization.
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spelling doaj-art-61d9a9df4fe4459486eeac3fb4e665eb2025-08-20T01:55:18ZengDavao Oriental State UniversityDavao Research Journal2244-44322984-71252018-12-01121192710.59120/drj.v12i1.1212Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explantSusan Labasano0Davao Oriental State College of Science and TechnologyTwo experiments were conducted to assess the effect of kinetin and NAA on the shoot growth and rooting of tissue-cultured Aglaonema tricolor using leaf bud explant. Likewise, it was also aimed to assess the suitability of Aglaonema leaf bud as explant and to determine the amount of NAA and kinetin needed in shoot formation and proliferation of the said foliage ornamental crop. NAA applied singly or in combination with kinetin did not affect the percentage survival of the explants, percent shoot formation, number of shoots, length of shoots, days to shoot formation and percent callus formation. Rates used were 0, 1.0 and 1.5 ppm kinetin and 0.25, and 5 ppm NAA A comparative evaluation of NAA and Kinetin was also conducted. In the follow-up study, percent callus formation, shoot length, number, and length of roots were significantly higher which showed a better effect with the application of 5 ppm NAA than 1.5 ppm Kinetin + 2.5 ppm NAA. In the first study, the optimum amount and the effective ratio of NAA and kinetin to enhance growth may not have been the ones tested. It is also possible that the variety used was not responsive to the growth regulator. The result of this study would add information on the use of growth regulators in the micropropagation of foliage crops. Studies like this will promote the advancement of foliage crop propagation for commercialization.https://davaoresearchjournal.ph/index.php/main/article/view/12shoot differentiationaglaonema tricolorkinetinnaphthalene acetic acidcytokinin
spellingShingle Susan Labasano
Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
Davao Research Journal
shoot differentiation
aglaonema tricolor
kinetin
naphthalene acetic acid
cytokinin
title Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
title_full Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
title_fullStr Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
title_full_unstemmed Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
title_short Application of Kinetin and Naphthalene Acetic Acid (NAA) for the growth and development of Aglaonema tricolor explant
title_sort application of kinetin and naphthalene acetic acid naa for the growth and development of aglaonema tricolor explant
topic shoot differentiation
aglaonema tricolor
kinetin
naphthalene acetic acid
cytokinin
url https://davaoresearchjournal.ph/index.php/main/article/view/12
work_keys_str_mv AT susanlabasano applicationofkinetinandnaphthaleneaceticacidnaaforthegrowthanddevelopmentofaglaonematricolorexplant